Difference between revisions of "Competition/InterLab Study/Flow Cytometry"

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<h2>Measurement with Flow Cytometry</h2>
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<p>This is a general guide for setting up your cells for flow cytometry readings in absolute units. These steps and questions are meant to provide a general protocol and we ask that you follow them to the best of your ability.
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<br><br>
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In order to carry out this measurement protocol, you will need:
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<li>A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP.  This channel will typically have a 488nm laser and a 530/30 filter, but the details may vary.</li>
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<li>Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for. </li>
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<b><i>All teams using a flow cytometer to measure GFP must finish the following items:</b></i>
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<li>Download the Excel File below (TeamName_InterLab_2017_Flow_Measurements.xls) and fill it out with your data. Rename the file [TeamName] to reflect your team's name.</li>
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<li>Email your completed Excel files to <i>measurement AT igem DOT org</i></li>
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<li>Fill in the two <b>Flow Cytometer Forms (Form 1 and Form 2)</b> provided at the bottom of this page</li>
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<li>Edit the InterLab study page on your team wiki (edit this page in your wikis: <i>https://2017.igem.org/Team:[TeamName]/InterLab</i></li>
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<h3>Flow Cytometer Forms</h3>
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Download the Excel Template file here: <a href="https://static.igem.org/mediawiki/2017/e/e0/TeamName_iGEM2017_Flow_Cytometry_Worksheet.xls">TeamName_iGEM2017_Flow_Cytometry_Worksheet.xls</a>
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<a href="https://goo.gl/forms/5n9N1l25PNWxZru32">
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      Form 1: Overview
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<a href="https://goo.gl/forms/mIEPVCFzn78erqU82">
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      Form 2: Culture Setup and Measurement
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Revision as of 19:57, 17 May 2017

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Measurement with Flow Cytometry

This is a general guide for setting up your cells for flow cytometry readings in absolute units. These steps and questions are meant to provide a general protocol and we ask that you follow them to the best of your ability.

In order to carry out this measurement protocol, you will need:

  • A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser and a 530/30 filter, but the details may vary.
  • Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for.


All teams using a flow cytometer to measure GFP must finish the following items:
  1. Download the Excel File below (TeamName_InterLab_2017_Flow_Measurements.xls) and fill it out with your data. Rename the file [TeamName] to reflect your team's name.
  2. Email your completed Excel files to measurement AT igem DOT org
  3. Fill in the two Flow Cytometer Forms (Form 1 and Form 2) provided at the bottom of this page
  4. Edit the InterLab study page on your team wiki (edit this page in your wikis: https://2017.igem.org/Team:[TeamName]/InterLab

Flow Cytometer Forms

Download the Excel Template file here: TeamName_iGEM2017_Flow_Cytometry_Worksheet.xls

Form 1: Overview
Form 2: Culture Setup and Measurement