Difference between revisions of "Competition/InterLab Study/Flow Cytometry"
| Line 36: | Line 36: | ||
<br> | <br> | ||
</p> | </p> | ||
| − | |||
</div> | </div> | ||
| + | |||
| + | <div class="clear"></div> | ||
<div class="column half_size"> | <div class="column half_size"> | ||
| − | |||
<div class="button"> | <div class="button"> | ||
| − | + | <a href="https://goo.gl/forms/5n9N1l25PNWxZru32"> | |
| + | Form 1: Overview | ||
| + | </a> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
| Line 51: | Line 52: | ||
<div class="column half_size"> | <div class="column half_size"> | ||
| − | |||
<div class="button"> | <div class="button"> | ||
| − | + | <a href="https://goo.gl/forms/mIEPVCFzn78erqU82"> | |
| + | Form 2: Culture Setup and Measurement | ||
| + | </a> | ||
</div> | </div> | ||
| − | + | ||
</div> | </div> | ||
Revision as of 19:58, 17 May 2017
Measurement with Flow Cytometry
This is a general guide for setting up your cells for flow cytometry readings in absolute units. These steps and questions are meant to provide a general protocol and we ask that you follow them to the best of your ability.
In order to carry out this measurement protocol, you will need:
- A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser and a 530/30 filter, but the details may vary.
- Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for.
All teams using a flow cytometer to measure GFP must finish the following items:
- Download the Excel File below (TeamName_InterLab_2017_Flow_Measurements.xls) and fill it out with your data. Rename the file [TeamName] to reflect your team's name.
- Email your completed Excel files to measurement AT igem DOT org
- Fill in the two Flow Cytometer Forms (Form 1 and Form 2) provided at the bottom of this page
- Edit the InterLab study page on your team wiki (edit this page in your wikis: https://2017.igem.org/Team:[TeamName]/InterLab
Flow Cytometer Forms
Download the Excel Template file here: TeamName_iGEM2017_Flow_Cytometry_Worksheet.xls
