Interlab.html

Fourth International Inter-Lab Measurement Study

 

 

This year Inter-lab Measurement Study aims to establish a GFP measurement protocol based on engineering principles for testing six RBS devices (BCDs) that are intended to make gene expression more precise and reliable  in labs around the world.

Team DEIAGRA had followed the plate reader protocol for this study as mentioned on (https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf) for obtaining the data represented below in form of graphs.

 

 

 

1.                   OD​600​ Reference point

 

 

	LUDOX	H2O
Replicate 1	0.0667	0.0473
Replicate 2	0.0617	0.0489
Replicate 3	0.0628	0.0515
Replicate 4	0.0668	0.0506
Arith. Mean	0.0645	0.049575
Corrected Abs600	0.014925
Reference OD600	0.0425
OD600/Abs600	2.8475712

 

 

 

 

 

 

 

 

 

 

 

2. Fluorescence Standard Curves

(uM Fluorescein)

 

 

 

3.Fluorescence Curve

FluoroscenceCurveReading (Colony 1)