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...for <i>MATa</i> cleavage. After selection, by homologous recombination, we deleted the Ura-tag for further usage. We selected the target colonies(<i>SynⅩdUr ...iated during the cell fusion in yeast mating process with Terminator being deleted. </p>

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...oid mate with each other. After selection, by homologous recombination, we deleted the <i>Ura-tag</i> for further usage. We selected the target colonies (<b>< ...text-align:center"><br/> Fig. 1-1. The PCR strategy for testing whether we deleted the HMRa in <b><i>SynⅩ-dUra</i></b>.

...process for MAT cleavage. After selection, by homologous recombination, we deleted the <i>Ura-tag</i> for further usage. We selected the target colonies (<b>< ...text-align:center"><br/> Fig. 1-1. The PCR strategy for testing whether we deleted the HMRa in <b><i>SynⅩ-dUra</i></b>.

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...mes involved in the expression of other quorum sensing molecules have been deleted. All these mutations make the strain JMH626 specific for detecting non-endo

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...contribute to the formation of curli nanofibres and will thus also remain deleted. Both pETDuet-1 and pET-28a, along with their inserts, will be transformed

chromosome to cause the gene to be replicated, inverted, displaced and deleted, gene between sites will be deleted. On the contrary when in the reverse direction,

...mes involved in the expression of other quorum sensing molecules have been deleted. All these mutations make the strain JMH626 specific for detecting non-endo

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