Difference between revisions of "Team:AFCM-Egypt"
Aymanshafei (Talk | contribs) |
Aymanshafei (Talk | contribs) |
||
| Line 162: | Line 162: | ||
</div> <!-- /.owl-carousel --> | </div> <!-- /.owl-carousel --> | ||
</section> | </section> | ||
| − | |||
| − | |||
<script src="https://2017.igem.org/Template:AFCM-Egypt/owl.carouseljs/javascript?action=raw&ctype=text/javascript"></script> | <script src="https://2017.igem.org/Template:AFCM-Egypt/owl.carouseljs/javascript?action=raw&ctype=text/javascript"></script> | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
| − | |||
$(document).ready(function() { | $(document).ready(function() { | ||
| Line 178: | Line 175: | ||
}); | }); | ||
| + | </body> | ||
| + | |||
</script> | </script> | ||
</html> | </html> | ||
{{Template:AFCM-Egypt/footer}} | {{Template:AFCM-Egypt/footer}} | ||
Revision as of 23:31, 16 October 2017
Abstract
Knock-in of Circular RNA gene in Hepatocellular Carcinoma cells via CRISPR/Cas9
Hepatocellular Carcinoma (HCC) is the leading cause of cancer deaths worldwide & ranked first among cancers
in males and next to breast cancer among females in Egypt - based upon results of National Cancer Registry Program of Egypt-.
"Grabbing the problem from the roots" is the best way to decently describe the use of CRISPR, a special gene editing technique
that we will be using to modulate a certain circRNA and adjust its gene expression, which is down-regulated in hepatocellular carcinoma.
This in consequence modifies miRNA expression thus amending the mRNA gene expression; which is the visible problem in our trials.
This will lead us to adopt a novel strategy for miRNA suppression by using circRNAs. This is accomplished by utilizing a synthetic circuit to give rise to a springboard in our battle against cancer.
Hepatocellular Carcinoma (HCC) is the leading cause of cancer deaths worldwide & ranked first among cancers in males and next to breast cancer among females in Egypt - based upon results of National Cancer Registry Program of Egypt-. "Grabbing the problem from the roots" is the best way to decently describe the use of CRISPR, a special gene editing technique that we will be using to modulate a certain circRNA and adjust its gene expression, which is down-regulated in hepatocellular carcinoma. This in consequence modifies miRNA expression thus amending the mRNA gene expression; which is the visible problem in our trials. This will lead us to adopt a novel strategy for miRNA suppression by using circRNAs. This is accomplished by utilizing a synthetic circuit to give rise to a springboard in our battle against cancer.
AFCM-Egypt
We strive to excel
click here
