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| <article> | | <article> |
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| <h3 class="major">Figure 2</h3> | | <h3 class="major">Figure 2</h3> |
| <p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FKBP, c-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FRB, n-Luc, and terminator.</p> | | <p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FKBP, c-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FRB, n-Luc, and terminator.</p> |
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| </article> | | </article> |
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| <h3 class="major">Figure 3</h3> | | <h3 class="major">Figure 3</h3> |
| <p>Our promoter-reporter system consists of two separate constructs each with a gene-specific promoter attached to a non-specific binding site fused to a domain of split luciferase. One construct consists of a COX-2 promoter, a FKBP domain, and c-Luc, and the other consists of a c-Myc promoter, a FRB domain, and n-Luc. The split system is built so that only when the presence of both COX-2 and c-Myc is detected will the binding sites be able to come together with the addition of rapamycin and cause glowing.</p> | | <p>Our promoter-reporter system consists of two separate constructs each with a gene-specific promoter attached to a non-specific binding site fused to a domain of split luciferase. One construct consists of a COX-2 promoter, a FKBP domain, and c-Luc, and the other consists of a c-Myc promoter, a FRB domain, and n-Luc. The split system is built so that only when the presence of both COX-2 and c-Myc is detected will the binding sites be able to come together with the addition of rapamycin and cause glowing.</p> |