Difference between revisions of "Team:ASIJ TOKYO/Results"
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<a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/e/e4/Anchipresentation.jpg" alt="" /></a> | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/e/e4/Anchipresentation.jpg" alt="" /></a> | ||
<div class="content"> | <div class="content"> | ||
| − | <h2 class="major"> Results </h2> | + | <h2 class="major"> Results Overview</h2> |
| + | <p>We attached the COX-2 (BBa_K2226003) and c-Myc (BBa_K2226002) promoters to the half of the reporter systems that yielded the best results, a method we took from the Peking iGEM team from 2015 (Peking iGEM Team 2015, 2015) (Rossi, Charlton, & Blau, 1994) (Rem, Galarneau, & Michnick, 2001). These reporter systems included the rapamycin-binding domain (FRB) fused to nLuc and the FK-506 binding protein 12 (FKBP) fused to cLuc. The fusion of the promoter to the reporter system made the nonspecific binding sites on the domains to become specific to the COX-2 (BBa_K2226003) and c-Myc (BBa_K2226004) proteins (Tomano & Nobuyuki, 2016). The newly specialized promoter-reporter system would then only come together in the presence of these two proteins. The creation of the FRB-rapamycin-FKBP complex induced the fusion of nLuc and cLuc, in turn generating light in the presence of rapamycin (Peking iGEM Team 2015, 2015). </p> | ||
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<h2 class="major">Promoter Strength Confirmation</h2> | <h2 class="major">Promoter Strength Confirmation</h2> | ||
| + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/b/bb/Result1.png" alt="" /></a> | ||
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<section class="features"> | <section class="features"> | ||
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<h2 class="major">Construction of the Promoter-Reporter System</h2> | <h2 class="major">Construction of the Promoter-Reporter System</h2> | ||
| + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/9/93/Result2.png" alt="" /></a> | ||
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<div class="inner"> | <div class="inner"> | ||
<h2 class="major">Proof of Concept</h2> | <h2 class="major">Proof of Concept</h2> | ||
| + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/3/34/Result3.png" alt="" /></a> | ||
| + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/c/c3/Result4.png" alt="" /></a> | ||
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<section class="features"> | <section class="features"> | ||
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<div class="content"> | <div class="content"> | ||
<h2 class="major">Confirmation</h2> | <h2 class="major">Confirmation</h2> | ||
| + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/4/49/Result5.png" alt="" /></a> | ||
| + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/2/2a/Result6.png" alt="" /></a> | ||
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</div> | </div> | ||
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Revision as of 13:27, 31 October 2017
Results Overview
We attached the COX-2 (BBa_K2226003) and c-Myc (BBa_K2226002) promoters to the half of the reporter systems that yielded the best results, a method we took from the Peking iGEM team from 2015 (Peking iGEM Team 2015, 2015) (Rossi, Charlton, & Blau, 1994) (Rem, Galarneau, & Michnick, 2001). These reporter systems included the rapamycin-binding domain (FRB) fused to nLuc and the FK-506 binding protein 12 (FKBP) fused to cLuc. The fusion of the promoter to the reporter system made the nonspecific binding sites on the domains to become specific to the COX-2 (BBa_K2226003) and c-Myc (BBa_K2226004) proteins (Tomano & Nobuyuki, 2016). The newly specialized promoter-reporter system would then only come together in the presence of these two proteins. The creation of the FRB-rapamycin-FKBP complex induced the fusion of nLuc and cLuc, in turn generating light in the presence of rapamycin (Peking iGEM Team 2015, 2015).
Promoter Strength Confirmation
Construction of the Promoter-Reporter System
Proof of Concept
Confirmation
