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Revision as of 20:17, 1 November 2017

Wiki_iGEM_Amazonas

DEMONSTRATE

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Toolbox modules assembly

How we constructed our circuits

core breakthroughs

  • Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;
  • Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;

    • CRISPeasy-out method (crOUT)

    core breakthroughs:

  • Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);
  • Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;