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| | </div> | | </div> |
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| − | <div>
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| − | <h2 style="margin-top: 0;padding-top: 1.5em">Toolbox modules assembly</h2>
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| − | <h4 style="padding: 0;">How we constructed our circuits</h4>
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| − | <h3 style="padding: 0;margin-bottom: 1.4em;">core breakthroughs</h3>
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| − | <li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li>
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| − | <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li>
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| − | </div>
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| − | <div>
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| − | <h1 style=" margin: 0;padding-top: 0.1em;"id="Testing"></h1>
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| − | <h2 style="margin-top: 0.5em;">CRISPeasy-out method (crOUT)</h2>
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| − | <h3 style="padding: 0;margin-top: 0.5em;margin-bottom: 0.5em;">core breakthroughs:</h3>
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| − | <li>Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);</li>
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| − | <li>Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;</li>
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| − | </div>
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| | <!-- <div style="background-color:#323232"> | | <!-- <div style="background-color:#323232"> |
