Difference between revisions of "Team:Amazonas Brazil/Demonstrate"

 
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       <ul class="Links_bar" style="background: linear-gradient(to left, #263D9E, #3F2759);">
 
       <ul class="Links_bar" style="background: linear-gradient(to left, #263D9E, #3F2759);">
 
         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
 
         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
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               <h2 class="Page_Title" align="middle">DEMONSTRATE</h2>
 
               <h2 class="Page_Title" align="middle">DEMONSTRATE</h2>
  
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           </div>
 
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               <div style="margin-bottom: 0;padding-bottom: 0; margin-top: 15px;">
 
               <div style="margin-bottom: 0;padding-bottom: 0; margin-top: 15px;">
  
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               <img alt="Erro" style="width: 70%;height: 70%; margin-left: 10%" usemap="#toolbox"  
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               <img alt="Erro" style="width: 70%;height: 70%; margin-left: 17%" usemap="#toolbox"  
 
               src="https://static.igem.org/mediawiki/2017/f/f8/Amazonas_Brazil_2017-10-31.png">
 
               src="https://static.igem.org/mediawiki/2017/f/f8/Amazonas_Brazil_2017-10-31.png">
  
 
               <map name="toolbox">
 
               <map name="toolbox">
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                 <area shape="rect" coords="321,3,788,336" href="" style="opacity: 1;"
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                 <area shape="rect" coords="321,3,788,336" href="#toolbox2"> <!--toolbox -->
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                 <area shape="rect" coords="9,603,398,835" href="" style="opacity: 1;"
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                 <area shape="rect" coords="9,603,398,835" href="#crout2"> <!--crout -->
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                 <area shape="rect" coords="709,607,1099,836" href="" style="opacity: 1;"
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                 <area shape="rect" coords="709,607,1099,836" href="#crin2"> <!--crin -->
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             <div>
 
             <div>
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              <a data-toggle="tab" data-target="#toolbox" id="toolbox2">
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              <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px;opacity: 1"
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              src="https://static.igem.org/mediawiki/2017/c/c2/Amazonas_Brazil_Demonstrate1_2.png"
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              onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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              onmouseout="this.style.opacity=1;this.filters.alpha.opacity=50"> <!-- Toolbox -->
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              </a>
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            </div>
  
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            <div class="tab-content" align="center">
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               src="https://static.igem.org/mediawiki/2017/c/c2/Amazonas_Brazil_Demonstrate1_2.png">
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              <div id="toolbox" class="team-content tab-pane fade" >
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</div>
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               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 60px"
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<div>
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               src="https://static.igem.org/mediawiki/2017/5/58/Amazonas_Brazil_Assembly.png">
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    <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 60px"
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              </div>
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               src="https://static.igem.org/mediawiki/2017/6/63/Amazonas_Brazil_Demonstrate3_1.png">
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            </div>
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    <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 60px"
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            <div>
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              <a data-toggle="tab" data-target="#crout" id="crout2">
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              <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 60px;opacity: 1"
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               src="https://static.igem.org/mediawiki/2017/6/63/Amazonas_Brazil_Demonstrate3_1.png"
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              onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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              onmouseout="this.style.opacity=1;this.filters.alpha.opacity=50"> <!-- CROUT -->
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              </a>
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            </div>
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            <div class="tab-content" align="center">
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              <div id="crout" class="team-content tab-pane fade" >               
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              <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 60px"
 
               src="https://static.igem.org/mediawiki/2017/0/0d/Amazonas_Brazil_Demonstrate4.png">
 
               src="https://static.igem.org/mediawiki/2017/0/0d/Amazonas_Brazil_Demonstrate4.png">
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    <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 15px"
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              <img alt="Erro" style="width: 60%;height: 80%;margin-left: 20%; margin-top: 60px"
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              src="https://static.igem.org/mediawiki/2017/8/85/Amazonas_Brazil_Demonstrate4_5.png">
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              <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 15px"
 
               src="https://static.igem.org/mediawiki/2017/e/ea/Amazonas_Brazil_Demonstrate5.png">
 
               src="https://static.igem.org/mediawiki/2017/e/ea/Amazonas_Brazil_Demonstrate5.png">
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</div>
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                 <div class="container-fluid" style="background-color:#323232; margin-bottom: 60px">
 
                 <div class="container-fluid" style="background-color:#323232; margin-bottom: 60px">
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<img style="width: 100%" src="https://static.igem.org/mediawiki/2017/a/a9/Amazonas_Brazil_Demonstrate_Proof_1.png">
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<img style="width: 100%" src="https://static.igem.org/mediawiki/2017/9/9a/Amazonas_Brazil_Demonstrate_Proof_2.png">
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                 <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 60px" src="https://static.igem.org/mediawiki/2017/f/f8/Amazonas_Brazil_Demonstrate6_1.png">
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                <img alt="Erro" style="width: 80%;height: 80%;margin-left: 8%; margin-top: 60px"
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    <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
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                src="https://static.igem.org/mediawiki/2017/3/3e/Amazonas_Brazil_Discussion.png">
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              src="https://static.igem.org/mediawiki/2017/d/d1/Amazonas_Brazil_Demonstrate7.png">
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    <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
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              </div>
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              <div>
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                <a data-toggle="tab" data-target="#crin" id="crin2">
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                 <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 60px;opacity: 1"  
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                    src="https://static.igem.org/mediawiki/2017/f/f8/Amazonas_Brazil_Demonstrate6_1.png"
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                    onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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                    onmouseout="this.style.opacity=1;this.filters.alpha.opacity=50"> <!-- CRIN -->
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                  </a>
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                <div class="tab-content" align="center">
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                  <div id="crin" class="team-content tab-pane fade" > 
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                  <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
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                    src="https://static.igem.org/mediawiki/2017/d/d1/Amazonas_Brazil_Demonstrate7.png">
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                <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
 
               src="https://static.igem.org/mediawiki/2017/f/fa/Amazonas_Brazil_Demonstrate8.png">
 
               src="https://static.igem.org/mediawiki/2017/f/fa/Amazonas_Brazil_Demonstrate8.png">
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    <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
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                <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
 
               src="https://static.igem.org/mediawiki/2017/3/34/Amazonas_Brazil_Demonstrate9.png">
 
               src="https://static.igem.org/mediawiki/2017/3/34/Amazonas_Brazil_Demonstrate9.png">
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    <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
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                <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%; margin-top: 40px"
 
               src="https://static.igem.org/mediawiki/2017/7/74/Amazonas_Brazil_Demonstrate10.png">
 
               src="https://static.igem.org/mediawiki/2017/7/74/Amazonas_Brazil_Demonstrate10.png">
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            </div>
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                <div class="container-fluid" style="background-color:#323232; margin-bottom: 60px">
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                  <img style="width: 100%" src="https://static.igem.org/mediawiki/2017/6/60/Amazonas_Brazil_Demonstrate_Proof_4.png">
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                  <img style="width: 100%" src="https://static.igem.org/mediawiki/2017/a/a2/Amazonas_Brazil_Demonstrate_Proof_5.png">
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                  <img style="width: 100%" src="https://static.igem.org/mediawiki/2017/3/3a/Amazonas_Brazil_Demonstrate_Proof_6.png">
 
                 </div>
 
                 </div>
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<div>
 
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                 <h2 style="margin-top: 0;padding-top: 1.5em">Toolbox modules assembly</h2>
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                 <img alt="Erro" style="width: 80%;height: 80%;margin-left: 8%; margin-top: 60px"
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                 <h4 style="padding: 0;">How we constructed our circuits</h4>
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                 src="https://static.igem.org/mediawiki/2017/2/2f/Amazonas_Brazil_Discussion2.png">
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                <h3 style="padding: 0;margin-bottom: 1.4em;">core breakthroughs</h3>
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                 </div>
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                <li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li>
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                 <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li>
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               </div>
 
               </div>
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              <div>
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              <h1 style=" margin: 0;padding-top: 0.1em;"id="Testing"></h1>
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                <h2 style="margin-top: 0.5em;">CRISPeasy-out method (crOUT)</h2>
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                <h3 style="padding: 0;margin-top: 0.5em;margin-bottom: 0.5em;">core breakthroughs:</h3>
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                <li>Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);</li>
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                <li>Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;</li>
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<script type="text/javascript" src="https://2017.igem.org/Template:Amazonas_Brazil/JS?action=raw&ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Template:Amazonas_Brazil/JS?action=raw&ctype=text/javascript"></script>
 
</body>
 
</body>
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<footer style="position:fixed;">
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<div class="subcontainer">
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/5/55/T--Amazonas_Brazil--IconTop.png" style="width: 4%;position: absolute;bottom: 0.5em;right: 0.5em;opacity: 0.5;"></a>
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</footer>
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<footer style="background: linear-gradient(to left, #263D9E, #3F2759)">
 
<footer style="background: linear-gradient(to left, #263D9E, #3F2759)">
 
   <div class="footer-container" >
 
   <div class="footer-container" >
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         <i class="fa fa-facebook" aria-hidden="true"></i>
 
     </a>
 
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/5/55/T--Amazonas_Brazil--IconTop.png" style="width: 4%;position: absolute;bottom: 5.5em;right: 0.5em;opacity: 0.5;"></a>
 
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Latest revision as of 03:24, 2 November 2017

Wiki_iGEM_Amazonas

DEMONSTRATE

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