Difference between revisions of "Team:Amazonas Brazil/Project"

 
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   <header>
 
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     <nav class="navbar navbar-fixed-top navbar-default" style="background-color: rgba(255, 255, 255, 0);">
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     <nav class="navbar navbar-fixed-top navbar-default" id="#" style="background-color: rgba(255, 255, 255, 0);">
 
       <ul class="Links_bar" style="background: linear-gradient(to left, #263D9E, #3F2759);opacity:0.75;">
 
       <ul class="Links_bar" style="background: linear-gradient(to left, #263D9E, #3F2759);opacity:0.75;">
 
         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
 
         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">Design</a></li>
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">Applied Design</a></li>
 
       </ul>
 
       </ul>
  
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                 <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto" src="https://static.igem.org/mediawiki/2017/7/7b/Amazonas_Brazil_Project_Roadmap.png" />
 
                 <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto" src="https://static.igem.org/mediawiki/2017/7/7b/Amazonas_Brazil_Project_Roadmap.png" />
  
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                 <div style='position:absolute;top:47%; left:45%;'>
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                 <div style='position:absolute;top:47%; left:44%;'>
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/HP/Gold_Integrated">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/HP/Gold_Integrated">
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                 </div>
 
                 </div>
  
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                 <div style='position:absolute;top:13%; left:45%;'>
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                 <div style='position:absolute;top:4%; left:44%;'>
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <a href="#Specifications">
 
                   <a href="#Specifications">
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                   </a>
 
                   </a>
 
                 </div>
 
                 </div>
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                 <div style='position:absolute;top:26%; left:67%;'>
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                 <div style='position:absolute;top:18%; left:66%;'>
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <a href="#Design">
 
                   <a href="#Design">
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                   </a>
 
                   </a>
 
                 </div>
 
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                 <div style='position:absolute;top:66%; left:67%;'>
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                 <div style='position:absolute;top:75%; left:66%;'>
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Model">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Model">
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                   </a>
 
                   </a>
 
                 </div>
 
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                 <div style='position:absolute;top:77%; left:45%;'>
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                 <div style='position:absolute;top:91%; left:44%;'>
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Assembly">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Assembly">
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                   </a>
 
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                   <!-- div para imagens que se sobrepoem -->
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Testing">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Testing">
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                   </a>
 
                   </a>
 
                 </div>
 
                 </div>
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                 <div style='position:absolute;top:27%; left:22%;'>
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                 <div style='position:absolute;top:19%; left:22%;'>
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <!-- div para imagens que se sobrepoem -->
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">
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               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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               src="https://static.igem.org/mediawiki/2017/4/4a/Amazonas_Brazil_Project_5_1.png">
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               src="https://static.igem.org/mediawiki/2017/b/b6/Amazonas_Brazil_Substitui%C3%A7%C3%A3o_Design.png">
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            <!--  <p class="p">Adicionar aqui</p> -->
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            <div style="margin-bottom:80px;" class="col-lg-12">
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            <div class="row">
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            <div class="col-lg-3" >
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            <a data-toggle="tab" data-target="#component1" >
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            <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
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            src="https://static.igem.org/mediawiki/2017/b/b2/Amazonas_Brazil_Component1.png"
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            onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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            onmouseout="this.style.opacity=1;this.filters.alpha.opacity=50">
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            </a>
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            </div>
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            <div class="col-lg-3" >
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            <a data-toggle="tab" data-target="#component2">
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            <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
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            src="https://static.igem.org/mediawiki/2017/4/4b/Amazonas_Brazil_Component2.png"
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            onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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            onmouseout="this.style.opacity=1;this.filters.alpha.opacity=50">
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            </a>
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            </div>
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            <div class="col-lg-3" >
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            <a data-toggle="tab" data-target="#component3">
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            <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
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            src="https://static.igem.org/mediawiki/2017/2/23/Amazonas_Brazil_Components_3.png"
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            onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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            onmouseout="this.style.opacity=1;this.filters.alpha.opacity=50">
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            </a>
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            </div>
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            <div class="col-lg-3" >
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            <a data-toggle="tab" data-target="#component4">
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            <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
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            src="https://static.igem.org/mediawiki/2017/c/ca/Amazonas_Brazil_Components_4.png"
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            onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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            onmouseout="this.style.opacity=1;this.filters.alpha.opacity=50">
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            </a>
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            </div>
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          </div>
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            <div class="tab-content" align="center">
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              <div id="component1" class="team-content tab-pane fade" >
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              <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/a/a6/Amazonas_Brazil_Cas9.png">
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              </div>
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              <div id="component2" class="team-content tab-pane fade" >
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              <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/6/63/Amazonas_Brazil_sgRNA.png">
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            <img class="img-responsive" src="https://static.igem.org/mediawiki/parts/b/b8/SgRNA_ESTRUTURA.png">
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              </div>
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              <div id="component3" class="team-content tab-pane fade" >
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              <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/24/RECA.png">
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              </div>
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              <div id="component4" class="team-content tab-pane fade" >
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              <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/23/Amazonas_Brazil_DonorDNA.png">
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              </div>
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            </div>
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          </div>
  
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  <p class="p">Adicionar aqui</p>
 
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               src="https://static.igem.org/mediawiki/2017/2/20/Amazonas_Brazil_Project_6_1.png">
 
               src="https://static.igem.org/mediawiki/2017/2/20/Amazonas_Brazil_Project_6_1.png">
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               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               src="https://static.igem.org/mediawiki/2017/5/5a/Amazonas_Brazil_Project_8_2.png">
 
               src="https://static.igem.org/mediawiki/2017/5/5a/Amazonas_Brazil_Project_8_2.png">
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            <img class="img-responsive" alt="Erro" style="width: 70%;height: 80%;margin-left: 10%"
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              src="https://static.igem.org/mediawiki/2017/d/db/CROUT.png">
  
 
  <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
  <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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               src="https://static.igem.org/mediawiki/2017/8/8e/Amazonas_Brazil_Project_9_1.png">  
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               src="https://static.igem.org/mediawiki/2017/8/8e/Amazonas_Brazil_Project_9_1.png">
  
 
<img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
<img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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             </div>
 
             </div>
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              <h2 style="margin-top: 1.4em;text-align: center;">Design</h2>
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              <h3 class="SubTitle">Check all the parts involved and our approaches with closer details!</h3>
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              <p class="p">
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                <h3 class="SubTitle">Cas9 (BBa_K2457001)</h3>
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                <p class="p">The standardized Cas9 device under control of AraC_pBAD regulatory module. In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to
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                  double-strand breaks. Through cell repair pathways, it will lay the road to genomic editing in living cells. </p>
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                <h3 class="SubTitle">Optimized sgRNA (BBa_K2457002)</h3>
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                <p class="p">This biobrick is constitutively expressed by J23100 promoter and codify an optimized sgRNA chimera composed of a protospacer crRNA with a GG motif at the 3’ end of its target-specific sequence, a tracrRNA with extended duplex length and
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                  a mutation at the thymine 4. It was rationally engineered to improve the editing efficiency of CRISPR-Cas9 machinery. When expressed, it guides the Cas9 nuclease activity through Rosalind-Watson-Crick base pairing complementarity with
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                  the target sequence. </p>
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                <h3 class="SubTitle">RecA (Bba_K2457003)</h3>
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                <p class="p">RecA is an essential building block part which leads the homologous recombination AND DNA insertion into the genome. After the target sequence cleavage catalyzed by the Cas9-sgRNA complex, the SOS response machinery is activated. As an
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                  output of the repair pathway, RecA builds a DNA-protein filament which investigates for a homologous template on undamaged DNA sequences, assisting in the invasion process and setting-up the Holliday Junction. This generates a crossover
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                  with the strands, leading to the recombination among the undamaged strand and the broken strand. </p>
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                DONOR DNA
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               </p>
 
               </p>
 
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            <div>
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              <img src="https://static.igem.org/mediawiki/2017/5/5f/T--Amazonas_Brazil--infografico_project.png" style="background-size: cover;  background-repeat: no-repeat;width:80%; margin-left: 10%;">
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            </div>
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           </div>
 
           </div>
 
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   <script type="text/javascript" src="https://2017.igem.org/Template:Amazonas_Brazil/JS?action=raw&ctype=text/javascript"></script>
 
   <script type="text/javascript" src="https://2017.igem.org/Template:Amazonas_Brazil/JS?action=raw&ctype=text/javascript"></script>
 
</body>
 
</body>
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<footer style="position:fixed;">
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<div class="subcontainer">
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/5/55/T--Amazonas_Brazil--IconTop.png" style="width: 4%;position: absolute;bottom: 0.5em;right: 0.5em;opacity: 0.5;"></a>
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</div>
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</footer>
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<footer style="background: linear-gradient(to left, #263D9E, #3F2759)">
 
<footer style="background: linear-gradient(to left, #263D9E, #3F2759)">
 
   <div class="footer-container">
 
   <div class="footer-container">
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         <i class="fa fa-facebook" aria-hidden="true"></i>
 
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     </a>
 
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    <a href="#"><img src="https://static.igem.org/mediawiki/2017/5/55/T--Amazonas_Brazil--IconTop.png" style="width: 4%;position: absolute;bottom: 5.5em;right: 0.5em;opacity: 0.5;"></a>
 
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