Difference between revisions of "Team:Amsterdam/test/thijs"
(Created page with "<html> <head> <meta charset="utf-8"/> <meta content="ie=edge" http-equiv="x-ua-compatible"/> <meta content="width=device-width, initial-scale=1, shrink-to-fit=no" name=...") |
|||
| Line 23: | Line 23: | ||
<div class="container-fluid"> | <div class="container-fluid"> | ||
<div class="navbar-header"> | <div class="navbar-header"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/d/d8/Pm_logo.png"/> | ||
<a class="navbar-brand nav-link" href="https://2017.igem.org/Team:Amsterdam/test/thijs"> | <a class="navbar-brand nav-link" href="https://2017.igem.org/Team:Amsterdam/test/thijs"> | ||
| − | + | Photosynthetic Magic | |
</a> | </a> | ||
</div> | </div> | ||
<ul class="list-inline nav navbar-right"> | <ul class="list-inline nav navbar-right"> | ||
<li class="list-inline-item dropdown"> | <li class="list-inline-item dropdown"> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/test/thijs/project"> |
Project | Project | ||
<span class="caret"> | <span class="caret"> | ||
| Line 70: | Line 71: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/test/thijs/outreach"> |
Outreach | Outreach | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</a> | </a> | ||
</li> | </li> | ||
| Line 133: | Line 129: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/test/thijs/attributions"> |
Attributions | Attributions | ||
</a> | </a> | ||
| Line 164: | Line 160: | ||
</div> | </div> | ||
<div class="section-button-container"> | <div class="section-button-container"> | ||
| − | <a class="section-button" href=" | + | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/test/thijs/project" id="Project"> |
| − | <img alt="Project | + | <img alt="Project" src="https://static.igem.org/mediawiki/2017/9/92/Project.png"> |
| − | + | <p> | |
| − | + | Project | |
| − | + | </p> | |
| + | </img> | ||
</a> | </a> | ||
<a class="section-button" href="#" id="Practices"> | <a class="section-button" href="#" id="Practices"> | ||
| − | <img alt=" | + | <img alt="Practices" src="https://static.igem.org/mediawiki/2017/6/62/Practices.png"/> |
<p> | <p> | ||
Practices | Practices | ||
| Line 177: | Line 174: | ||
</a> | </a> | ||
<a class="section-button" href="#" id="Lab"> | <a class="section-button" href="#" id="Lab"> | ||
| − | <img alt=" | + | <img alt="Lab" src="https://static.igem.org/mediawiki/2017/0/0c/Lab.png"/> |
<p> | <p> | ||
Lab | Lab | ||
</p> | </p> | ||
</a> | </a> | ||
| − | <a class="section-button" href=" | + | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/test/thijs/team" id="Team"> |
| − | <img alt=" | + | <img alt="Team" src="https://static.igem.org/mediawiki/2017/3/32/Team.png"/> |
<p> | <p> | ||
Team | Team | ||
| Line 189: | Line 186: | ||
</a> | </a> | ||
<a class="section-button" href="#" id="Achievements"> | <a class="section-button" href="#" id="Achievements"> | ||
| − | <img alt=" | + | <img alt="Achievements" src="https://static.igem.org/mediawiki/2017/6/64/Achievements.png"/> |
<p> | <p> | ||
Achievements | Achievements | ||
| Line 195: | Line 192: | ||
</a> | </a> | ||
</div> | </div> | ||
| − | <div class="home- | + | <div class="home-project-container-outer"> |
| − | + | <div class="home-project-container"> | |
| − | + | <div class="logo-spacer alternate"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/c/cb/Logo_line1.png"/> | |
| − | + | ||
| − | + | ||
| − | <div class=" | + | |
| − | < | + | |
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | + | <div class="line-connector-container"> | |
| − | + | <div class="line-connector" id="first"> | |
| − | + | </div> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | + | <div class="home-content-container"> | |
| − | + | <div class="home-intro-container" id="first"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/6/62/Practices.png"/> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p> | <p> | ||
| − | + | We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO2. | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="home-content-container"> |
| − | < | + | <div class="line-connector" id="second"> |
| − | + | </div> | |
| − | + | <div class="home-intro-container" id="second"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/7/70/Recycle.png"/> | |
| − | + | ||
<p> | <p> | ||
| − | + | But what if CO2 were a resource, rather than a wasteful pollutant; and might even replace oil? | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="line-connector-container"> |
| − | < | + | <div class="line-connector" id="third"> |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="home-content-container"> | |
| − | + | <div class="home-intro-container" id="first"> | |
| + | <img src="https://static.igem.org/mediawiki/2017/4/41/Plants.png"/> | ||
<p> | <p> | ||
| − | + | Traditionally people have tried to achieve this by producing fuels and sugars with plants, and by fermenting these sugars with bacteria to produce other chemicals. | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="home-content-container"> |
| − | < | + | <div class="line-connector" id="second"> |
| − | + | </div> | |
| − | + | <div class="home-intro-container" id="second"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/9/92/Project.png"/> | |
| − | + | ||
<p> | <p> | ||
| − | + | But there is a better way, where no arable land is needed and which is faster and more efficient. This is the way our team has chosen to go, using cyanobacteria to take up CO2 and directly convert it into the valuable chemical | |
| − | < | + | <span style="color: rgba(88, 190, 190, 1)"> |
| − | + | FUMARATE | |
| − | + | </span> | |
| − | </ | + | |
</p> | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="line-connector-container"> | ||
| + | <div class="line-connector" id="third"> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="vertical-text-container-outer" id="home"> |
<div class="vertical-text-container"> | <div class="vertical-text-container"> | ||
<p class="vertical-text"> | <p class="vertical-text"> | ||
| − | + | PROJECT | |
</p> | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <div class="info-oval-container" id="home"> | ||
| + | <div class="line-connector-container"> | ||
| + | <div class="line-connector" id="fourth"> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="info-oval"> | ||
| + | <div class="left-semi-circle"> | ||
| + | </div> | ||
| + | <p> | ||
| + | <b> | ||
| + | Did you know that.... | ||
| + | </b> | ||
| + | <br/> | ||
| + | Fumarate is used to make certain plastics, food additives, and medicine and is currently made from petroleum. | ||
| + | </p> | ||
| + | <div class="right-semi-circle"> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="home-gif-container"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/8/88/Logo_gif.gif"/> | ||
| + | </div> | ||
| + | <!-- <div class="home-spacer"> | ||
| + | <img src='images/design_thingy_1.png'> | ||
| + | </div> | ||
| + | <div class="summary-container"> | ||
| + | <div class="summary-col-left"> | ||
| + | |||
| + | </div> | ||
| + | <div class="summary-col-mid"> | ||
| + | <p class="summary-text">We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO<sub>2</sub>. But what if CO<sub>2</sub> were a resource, rather than a wasteful pollutant; and might even replace oil? We aspire to create a bio-based economy – one that meets its energy and production demands by leveraging biotechnology [1]. We can genetically engineer bacteria to produce a compound we need. This is what we call a bacterial ‘cell factory’. | ||
| + | Meet <a class="in-text-link" href="#" style="font-size: 24px">CYANOFACTORY</a>, a cyanobacterium that stably and efficiently produces fumarate.</p> | ||
| + | </div> | ||
| + | <div class="summary-col-right"> | ||
| + | <div class="vertical-text-container"> | ||
| + | <p class='vertical-text'>OUR GOAL</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="home-spacer"> | ||
| + | <img src='images/design_thingy_1.png'> | ||
| + | </div> | ||
| + | <div class="summary-container"> | ||
| + | <div class="summary-col-left"> | ||
| + | <img src="images/erlenmeyer_flask.png" width="200px" height="260px"> | ||
| + | </div> | ||
| + | <div class="summary-col-mid"> | ||
| + | <p class="summary-text">We use the cyanobacterium Synechocystis PCC6803, a photosynthetic model organism of which the genetic toolbox is rapidly expanding.<br><br> | ||
| + | <a class="in-text-link" href="#">Click here for a live feed with our Synechocystis cultivators.</a></p> | ||
| + | </div> | ||
| + | <div class="summary-col-right"> | ||
| + | <div class="vertical-text-container"> | ||
| + | <p class='vertical-text'>SYNECHOCYSTIS</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="home-spacer"> | ||
| + | <img src='images/design_thingy_1.png'> | ||
| + | </div> | ||
| + | <div class="summary-container"> | ||
| + | <div class="summary-col-left"> | ||
| + | <img src="images/fumarate.png" width="200px" height="202px"> | ||
| + | </div> | ||
| + | <div class="summary-col-mid"> | ||
| + | <p class="summary-text">Fumarate is a multifaceted acid that is used as a plastic precursor, an additive for the food industry and a drug against multiple sclerosis and psoriasis.<br><br> | ||
| + | <a class="in-text-link" href="#">Click here to see what we did with Fumarate.</a></p> | ||
| + | </div> | ||
| + | <div class="summary-col-right"> | ||
| + | <div class="vertical-text-container"> | ||
| + | <p class='vertical-text'>FUMARATE</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div id="modules" class="summary-container"> | ||
| + | <div id="modules" class="home-spacer"> | ||
| + | <img src='images/design_thingy_1_long.png'> | ||
| + | </div> | ||
| + | <div class="summary-col-left"> | ||
| + | |||
| + | </div> | ||
| + | <div class="summary-col-mid"> | ||
| + | <div id='sensor' class="summary-modules-outer"> | ||
| + | <img class="animated-module-icon" src='images/sensor_icon.png'> | ||
| + | <div class="modules-summary-text"> | ||
| + | <h1>Biosensor</h1> | ||
| + | <p>A biosensor for fumarate is constructed in order to facilitate a high throughput screening of extracellular fumarate. This is essential for low measurement time and costs. | ||
| + | <br><a class="in-text-link" href="#">>>read more</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div id="transporter" class="summary-modules-outer"> | ||
| + | <img class="animated-module-icon" src='images/transporter_icon.png'> | ||
| + | <div class="modules-summary-text"> | ||
| + | <h1>Transporter</h1> | ||
| + | <p>In anticipation of high fumarate production, transport of fumarate out of the cell can be a limiting factor. This mechanism is largely unknown in Synechocystis and will therefore be -guided by bioinformatics- characterized by means of knock-out and over- expression experiments. | ||
| + | <br><a class="in-text-link" href="#">>>read more</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div id="shunt" class="summary-modules-outer"> | ||
| + | <img class="animated-module-icon" src='images/shunt_icon.png'> | ||
| + | <div class="modules-summary-text"> | ||
| + | <h1>Glyoxylate Shunt</h1> | ||
| + | <p>We will incorporate two enzymes (the glyoxylate shunt) in the Synechocystis genome, creating a shortcut in the TCA-cycle. Our modeling results show that if this shunt is only expressed at night, and the pentose phosphate pathway is blocked by knocking out the zwf gene, more fumarate will be produced. This means we will create a unique combination of growth- coupled and inducible production. | ||
| + | <br><a class="in-text-link" href="#">>>read more</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div id="stability" class="summary-modules-outer"> | ||
| + | <img class="animated-module-icon" src='images/stability_icon.png'> | ||
| + | <div class="modules-summary-text"> | ||
| + | <h1>Stability</h1> | ||
| + | <p>When an organism is genetically modified to create a product, it causes a decrease in growth rate. This means that a mutated - non- producing - organism will grow faster and take over the population. This problem is tackled by coupling fumarate production to growth. Given that evolution selects heavily on growth rate, we now have a way of naturally selecting for production rate [4]! Knocking out the fumarate degrading reaction in the TCA cycle causes fumarate to be produced in a growth coupled way. | ||
| + | <br><a class="in-text-link" href="#>">>>read more</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="summary-col-right"> | ||
| + | <div class="vertical-text-container"> | ||
| + | <p class='vertical-text'>MODULES</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | --> | ||
</div> | </div> | ||
<div class="footer"> | <div class="footer"> | ||
<div class="footer-col-left"> | <div class="footer-col-left"> | ||
<a href="https://www.aimms.vu.nl/en/index.aspx"> | <a href="https://www.aimms.vu.nl/en/index.aspx"> | ||
| − | <img alt=" | + | <img alt="Aimms1" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/3/36/Aimms1.png"> |
</img> | </img> | ||
</a> | </a> | ||
| Line 381: | Line 409: | ||
</a> | </a> | ||
<a href="https://www.matrixic.nl/"> | <a href="https://www.matrixic.nl/"> | ||
| − | <img alt="Matrix" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/8/84/Matrix.png" | + | <img alt="Matrix" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/8/84/Matrix.png"/> |
| − | + | ||
</a> | </a> | ||
<a href="https://2017.igem.org/Team:Amsterdam/test/thijs/www.merckmillipore"> | <a href="https://2017.igem.org/Team:Amsterdam/test/thijs/www.merckmillipore"> | ||
| − | <img alt="Merck" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/d/dc/Merck.png" | + | <img alt="Merck" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/d/dc/Merck.png"/> |
| − | + | ||
</a> | </a> | ||
<a href="http://phenomenex.com/"> | <a href="http://phenomenex.com/"> | ||
| − | <img alt="Phenomenex" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/a/af/Phenomenex.png" | + | <img alt="Phenomenex" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/a/af/Phenomenex.png"/> |
| − | + | ||
</a> | </a> | ||
<a href="http://www.snapgene.com/"> | <a href="http://www.snapgene.com/"> | ||
| − | <img alt="Snapgene" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/8/8d/Snapgene.png" | + | <img alt="Snapgene" class="footer-sponsor-img" src="https://static.igem.org/mediawiki/2017/8/8d/Snapgene.png"/> |
| − | + | ||
</a> | </a> | ||
<a href="http://sils.uva.nl/"> | <a href="http://sils.uva.nl/"> | ||
Latest revision as of 13:03, 12 October 2017
We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO2.
But what if CO2 were a resource, rather than a wasteful pollutant; and might even replace oil?
Traditionally people have tried to achieve this by producing fuels and sugars with plants, and by fermenting these sugars with bacteria to produce other chemicals.
But there is a better way, where no arable land is needed and which is faster and more efficient. This is the way our team has chosen to go, using cyanobacteria to take up CO2 and directly convert it into the valuable chemical FUMARATE
PROJECT
Did you know that....
Fumarate is used to make certain plastics, food additives, and medicine and is currently made from petroleum.
