Attributions

In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.

• • Diethart Mattanovich, Michael Sauer and Hans Marx: organization of the iGEM project
• • Gabi Wilt: ordered Materials for our Team
• • Corinna Rebnegger: helped us doing flow cytometric experiments for characterization of our pGAL syn
• • Tom Gaßler: advice on setting up our CRISPR/Cas9 mediated gene integration in Saccharomyces cerevisiae by supporting us with primers for assembling the specific sgRNAs.
• • Martin Altvater: provided us with different plasmids for Saccharomyces cerevisiae ready for our Golden Gate system
• • Jürgen Zanghelini: supported us with modeling
• • Florian Strobl: advice and lab material
• • Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids
• • Franziska Doleschal: organized our bank account and managed all our invoices
• • Anna Gindl: designed our Logo
• • Nikolaus Huber: helped us setting up the core code of our wiki page
• • Sauer group: Provided us BB1_01 – BB1_08
• • Mattanovich/ Gasser group: provided us material, all empty BBs for the Golden GATE system and many protocols:
  • • Preparation chemical competent E. coli
  • • Transformation chemical competent E. coli
  • • Preparation of electro competent S. cerevisiae
  • • Transformation of electro competent S. cerevisiae
  • • mRNA extraction
• • Bernd Albrecht: helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters.

Furthermore we want to thank Matthias Steiger, Stefanie Wiesauer,