Difference between revisions of "Team:BOKU-Vienna/Attributions"

 
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                 <h2>Attributions.</h2>
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                 <h2>Attributions</h2>
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                 <p>
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<p>In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.</p>
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<li>Gabi Wilt: ordered Materials for our Team
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<p>
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</li><li>Corina Rebnegger: helped us doing Flow cytometric experiments for characterization of our pGAL syn  
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<ul class="ulclass">
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</li><li>Tom Gassler: gived us advice for setting up our CRISPR Cas9 mediated gene integration in <i> Saccharomyces cerevisiae </i> by supporting us with primers for assembling the specific sgRNAs.
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<li>•  <a href="https://forschung.boku.ac.at/fis/suchen.person_uebersicht?sprache_in=en&menue_id_in=101&id_in=441" target="_blank">Diethard Mattanovich,</a> <a href="https://forschung.boku.ac.at/fis/suchen.person_uebersicht?sprache_in=en&ansicht_in=&menue_id_in=101&id_in=1307" target="_blank">Brigitte Gasser,</a> <a href="https://forschung.boku.ac.at/fis/suchen.person_uebersicht?id_in=5812&menue_id_in=101&sprache_in=de" target="_blank"> Michael Sauer</a>and Hans Marx: organization of the iGEM project
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</li><li>Martin Altvater: provided us different plasmids for <i> Saccharomyces cerevisiae </i> ready for our Golden Gate system  
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</li><li>• Gabi Wilt ordered materials for our team
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</li><li>Jürgen Zanghelini: supported us with our modelling approaches
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</li><li>• Corinna Rebnegger helped us doing flow cytometric experiments for characterization of our pGAL syn  
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</li><li>Florian Strobl:
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</li><li>• Tom Gaßler advice on setting up our CRISPR/Cas9 mediated gene integration in <i> S. cerevisiae </i> by supporting us with primers for assembling the specific sgRNAs <nobr> (adapted from <a href="https://doi.org/10.1016/j.meteno.2015.03.001" target="_blank">Stovicek 2015).</a></nobr>
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</li><li>Gerald Striedner and Esther Egger provided us the plasmids pSIM 5 and pSIM 9.
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</li><li>• Martin Altvater: provided us with different plasmids for <i> Saccharomyces cerevisiae </i> ready for our Golden Gate system  
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</li><li>Brigitte Lang
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</li><li>• Jürgen Zanghellini supported us with modeling
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</li><li>Matthias Steiger
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</li><li>• Florian Strobl advice and lab material
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</li><li>Franziska Doleschal: organized our bank account and managed all our invoices.
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</li><li>• Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids
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</li><li>Anna Gindl: designed our Logo
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</li><li>• Franziska Doleschal organized our bank account and managed all our invoices
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</li><li>Nikolaus Huber: helped us setting up the core code of our Wiki page
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</li><li>• Anna Gindl designed our Logo
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</li><li>AG Sauer: Provided us BB1_01 – BB1_08  
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</li><li>• Nikolaus Huber helped us setting up the core code of our wiki page
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</li><li>AG Mattanovich/Gasser: provided us material, all empty BBs for the Golden GATE system and many protocols:  
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</li><li>• Sauer group provided us BB1_01 – BB1_08  
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<ul><li>Preparation of chemical competent <i>E. coli</i>
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</li><li>• Mattanovich/ Gasser group provided us material, all empty BBs for the Golden Gate system and many protocols:  
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</li><li>Transformation chemical competent <i>E. coli</i>
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<ul class="ulclass">
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</li><li>Preparation of electro competent <i>S. cerevisiae</i>
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        <li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#CompetentColi">Preparation chemical competent <i>E. coli</i></a>
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</li><li>Transformation of electro competent <i>S. cerevisiae<i/>
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</li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#TransformationColi">Transformation chemical competent <i>E. coli</i></a>
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</li><li>mRNA extraction</li><ul></li>
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</li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#PreparationYeast">Preparation of electro competent <i>S. cerevisiae</i></a>
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<li>Bernd Albrecht: helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters. </li></ul>
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</li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#TransformationYeast">Transformation of electro competent <i>S. cerevisiae</i></a>
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</li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#mRNA">mRNA extraction</li></ul></li></a>
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<li>• Bernd Albrecht helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters.
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</li><li> • STV LBT helped us organizing the "Spritzerstand"</li></ul>
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</p>
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<p> Furthermore, we want to thank Matthias Steiger, Alexandra Graf, Stefanie Wiesauer, Christiane Veit, Gordana Wozniak-Knopp, Siniša Petrik, Roland Prielhofer, Hannes Rußmayer, Michael Egermeier, Valeria Ellena, Lina Heistinger, Dominik Jeschek, Richard Zahrl, Ursula Kieswetter and many more!</p>
  
  
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Latest revision as of 13:10, 22 November 2017

Attributions

V

Attributions

In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.

  • • Diethard Mattanovich, Brigitte Gasser, Michael Sauerand Hans Marx: organization of the iGEM project
  • • Gabi Wilt ordered materials for our team
  • • Corinna Rebnegger helped us doing flow cytometric experiments for characterization of our pGAL syn
  • • Tom Gaßler advice on setting up our CRISPR/Cas9 mediated gene integration in S. cerevisiae by supporting us with primers for assembling the specific sgRNAs (adapted from Stovicek 2015).
  • • Martin Altvater: provided us with different plasmids for Saccharomyces cerevisiae ready for our Golden Gate system
  • • Jürgen Zanghellini supported us with modeling
  • • Florian Strobl advice and lab material
  • • Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids
  • • Franziska Doleschal organized our bank account and managed all our invoices
  • • Anna Gindl designed our Logo
  • • Nikolaus Huber helped us setting up the core code of our wiki page
  • • Sauer group provided us BB1_01 – BB1_08
  • • Mattanovich/ Gasser group provided us material, all empty BBs for the Golden Gate system and many protocols:
  • • Bernd Albrecht helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters.
  • • STV LBT helped us organizing the "Spritzerstand"

Furthermore, we want to thank Matthias Steiger, Alexandra Graf, Stefanie Wiesauer, Christiane Veit, Gordana Wozniak-Knopp, Siniša Petrik, Roland Prielhofer, Hannes Rußmayer, Michael Egermeier, Valeria Ellena, Lina Heistinger, Dominik Jeschek, Richard Zahrl, Ursula Kieswetter and many more!