Line 2: | Line 2: | ||
<html> | <html> | ||
<style> | <style> | ||
+ | h2{ text-transform: none !important;} | ||
.myintro { | .myintro { | ||
display: table; | display: table; |
Revision as of 14:15, 22 October 2017
GFP
The Golden Gate modular cloning system we worked with provides many advantages over other assembling standards. However, the wildly used GFP BBa_E0040 part is not compatible with this standard (RFC[1000]). Therefore, we decided to improve this part by silently mutating a BsaI restriction site to comply with RFC[1000]. Additionally we added the special RFC[1000] prefix and suffix with BsaI restriction sites. After digestion they create so called fusion sites, sticky ends which are specific for coding sequences: AATG at the 5’ end and GCTT at the 3’ end .
The new, RFC[1000] compatible part BBa_K2294444 was cloned into the pSB1C3 shipping vector.
FLPe + NLS
The new, RFC[10] compatible part BBa_K2294000 was cloned into the pSB1C3 shipping vector.