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Revision as of 14:17, 22 October 2017
Improve
GFP
The Golden Gate modular cloning system we worked with provides many advantages over other assembling standards. However, the wildly used GFP BBa_E0040 part is not compatible with this standard (RFC[1000]). Therefore, we decided to improve this part by silently mutating a BsaI restriction site to comply with RFC[1000]. Additionally we added the special RFC[1000] prefix and suffix with BsaI restriction sites. After digestion they create so called fusion sites, sticky ends which are specific for coding sequences: AATG at the 5’ end and GCTT at the 3’ end .
The new, RFC[1000] compatible part BBa_K2294444 was cloned into the pSB1C3 shipping vector.
FLPe + NLS
The new, RFC[10] compatible part BBa_K2294000 was cloned into the pSB1C3 shipping vector.
