Team:BOKU-Vienna/Improve

Improve

V

GFP


The Golden Gate modular cloning system we worked with provides many advantages over other assembling standards. However, the wildly used GFP BBa_E0040 part is not compatible with this standard (RFC[1000]). Therefore, we decided to improve this part by silently mutating a BsaI restriction site to comply with RFC[1000]. Additionally we added the special RFC[1000] prefix and suffix with BsaI restriction sites. After digestion they create so called fusion sites, sticky ends which are specific for coding sequences: AATG at the 5’ end and GCTT at the 3’ end .

The new, RFC[1000] compatible part BBa_K2294444 was cloned into the pSB1C3 shipping vector.

FLPe + NLS


The new, RFC[10] compatible part BBa_K2294000 was cloned into the pSB1C3 shipping vector.