Modeling.

With D.I.V.E.R.T. it is possible to create a large library of a mutant gene in vivo. An important part of creating a library is the amount of mutations introduced in this gene. Few mutations may not be enough to change a gene’s property, a huge amount of mutations, however, can lead to unfavorable effects regardless of small positive influences. Thus, the exact knowledge of the distribution of mutations is vital for creating functional libraries with D.I.V.E.R.T. Underneath, we are modeling how the distribution of clones containing a different amount of mutations changes over time and how this distribution is affected by a different growth rate µ and mutation rate f.