Revision as of 19:26, 28 October 2017

Notebook

All protocols we used for the methods can be found here: Protocol

Week 1 (03/07-07/07)

Week 2 (10/07-14/07)

Week 3 (17/07-21/07)

Week 4 (24/07-28/07)

Week 5 (31/07-04/08)

Week 6 (07/08-11/08)

Week 7 (14/08-18/08)

Week 8 (21/08-25/08)

Week 9 (28/08-01/09)

Week 10 (04/09-08/09)

Week 11 (11/09-15/09)

Week 12 (18/09-22/09)

Week 13 (25/09-29/09)

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 <br>• PCR: 1, 2, 3, 4, 6, 15, 18, 20, 23, 24, 25, 26</br>
 Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol" target="_blank">protocol</a>.
-<br><br>• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01(unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)</br></br>
+<br><br>• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01(unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)</br>
-GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.
+GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 <br><br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
 <br>• Transformation of HSM174 + pSIM5 (unsuccessful)</br>