Difference between revisions of "Team:BOKU-Vienna/Notebook"

 
(340 intermediate revisions by 5 users not shown)
Line 5: Line 5:
 
<!--header-->
 
<!--header-->
 
<style>
 
<style>
−
   .mydiv {
+
   .yolo {
−
      margin: 20% !important; }
+
    width: 765px;
 +
    margin-left: 16.5%;
 +
    }
 +
  .arrows{text-decoration:none;color:#170 !important;
 +
    }  
 
   .myintro {
 
   .myintro {
 
       display: table;
 
       display: table;
Line 32: Line 36:
 
       font-family: Tahoma, Geneva, sans-serif;
 
       font-family: Tahoma, Geneva, sans-serif;
 
     }
 
     }
−
 
  
 
     .mylist {
 
     .mylist {
−
    text-align: left;
+
      list-style: round url("sqpurple.gif")
−
    margin: 20% !important;
+
 
     }
 
     }
 
      
 
      
Line 60: Line 62:
 
     }
 
     }
  
−
a.anchor {
+
    a.anchor {
 
     display: block;
 
     display: block;
 
     position: relative;
 
     position: relative;
 
     top: -250px;
 
     top: -250px;
 
     visibility: hidden;
 
     visibility: hidden;
−
}
+
    }
 +
 
 +
    pre {
 +
    overflow: auto;
 +
    padding: 0.5em;
 +
    width: 765px;
 +
    background-color: #eef6fc;
 +
    color: #110903;
 +
    margin-left: 16.5%;
 +
    font-family: Tahoma, Geneva, sans-serif;
 +
    transition: all .4s ease;
 +
 
 +
    }
 +
 +
    pre:hover {
 +
    background-color: #6499cd;
 +
    position: relative;
 +
    padding: 1em;
 +
    transition: all .4s ease;
 +
    }
 +
 
 +
.modalDialog {
 +
      position: fixed;
 +
      font-family: Arial, Helvetica, sans-serif;
 +
      top: 0;
 +
      right: 0;
 +
      bottom: 0;
 +
      left: 0;
 +
      background: rgba(0, 0, 0, 0.8);
 +
      z-index: 99999;
 +
      opacity:0;
 +
      -webkit-transition: opacity 400ms ease-in;
 +
      -moz-transition: opacity 400ms ease-in;
 +
      transition: opacity 400ms ease-in;
 +
      pointer-events: none;
 +
    }
 +
    .modalDialog:target {
 +
      opacity:1;
 +
      pointer-events: auto;
 +
    }
 +
    .modalDialog > div { 
 +
      width: 45%;
 +
      position: relative;
 +
      margin: 5% auto;
 +
      padding: 0px 0px 0px 0px;
 +
      border-radius: 10px;
 +
      background: #fff;
 +
    }
 +
    .close {
 +
      background: white !important;
 +
      color: black !important;
 +
      opacity: 1 !important;
 +
      line-height: 25px;
 +
      position: absolute;
 +
      right: -13px;
 +
      text-align: center;
 +
      top: -10px;
 +
      width: 24px;
 +
      text-decoration: none;
 +
      font-weight: bold;
 +
      -webkit-border-radius: 12px;
 +
      -moz-border-radius: 12px;
 +
      border-radius: 12px;
 +
      -moz-box-shadow: 1px 1px 3px #000 !important;
 +
      -webkit-box-shadow: 1px 1px 3px #000 !important;
 +
      box-shadow: none;
 +
      text-shadow: none !important;
 +
 
 +
    }
 +
    .close:hover {
 +
      background: #6499cd !important;
 +
      box-shadow: none;
 +
      text-shadow: none !important;
 +
   
 +
    }
 +
   
  
  
 
</style>
 
</style>
 +
 +
<script type="text/javascript">
 +
<!--
 +
    function expand_collapse(id) {
 +
      var e = document.getElementById(id);
 +
      var f = document.getElementById(id+"_arrows");
 +
      if(e.style.display == 'none'){
 +
          e.style.display = 'block';
 +
          f.innerHTML = 'hidden';
 +
      }
 +
      else {
 +
          e.style.display = 'none';
 +
          f.innerHTML = 'hidden';
 +
      }
 +
    }
 +
 +
//-->
 +
</script>
 +
  
  
Line 99: Line 195:
 
                         <a class="page-scroll" href="#Notebook">Notebook</a>
 
                         <a class="page-scroll" href="#Notebook">Notebook</a>
 
                     </li>
 
                     </li>
−
                 
+
                                   
 
                 </ul>
 
                 </ul>
 
             </div>
 
             </div>
Line 130: Line 226:
 
             <div class="col-lg-8 col-lg-offset-2">
 
             <div class="col-lg-8 col-lg-offset-2">
 
                 <h2>Notebook.</h2>
 
                 <h2>Notebook.</h2>
−
                 <p> <br>Our Notebook is still under construction. You can find our protocol for the methods here:  
+
                 <p> <br>All protocols we used for the methods can be found here:  
−
<a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol">Protocol</a>
+
<a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol" target="_blank">Protocol</a>
−
                  
+
                 </br>
−
 
+
                    <br>For further information regarding our Golden Gate Assembly products, we refer to our <a href="https://2017.igem.org/Team:BOKU-Vienna/Part_Collection" target="_blank">part collection</a> site. The list of our primers can be found <a href="https://static.igem.org/mediawiki/2017/e/ea/T--BOKU-Vienna--Primerliste.pdf" target="_blank">here</a>.
−
 
+
</p>
−
</br></p>
+
 
             </div>
 
             </div>
 
         </div>
 
         </div>
  
−
<!---TEST--->
+
<a href="#week1" id="scrollnav" class="arrows" onclick="expand_collapse('1');"><pre><font size="+2"><b>Week 1</b></font> (03/07-07/07)</pre></a><span style="font-size:150%;"><b>  </b></span>
−
<body>
+
<div id="1" class="yolo" style="display:none;">
−
<p></p>
+
  <p>
−
<p></p>
+
<br>  • Preparation of growth media </br>
−
<p></p>
+
<br>   • Interlab Challenge 2017: Transformations of the plasmids into <i>E. coli</i> DH5α. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site. </br>
 +
<br>   • <i>E. Coli</i> DH10B cells were made chemically competent.</br>
 +
<br>  • Miniprep of GG plasmids:
 +
<a href="#BB1_01" class="bgscroll"> BB1_01</a>, <a href="#BB1_02" class="bgscroll"> BB1_02</a>, <a href="#BB1_03" class="bgscroll"> BB1_03</a>, <a href="#BB1_04" class="bgscroll"> BB1_04</a>, <a href="#BB1_05" class="bgscroll"> BB1_05</a>, <a href="#BB1_06" class="bgscroll"> BB1_06</a>, <a href="#BB1_07" class="bgscroll"> BB1_07</a>, <a href="#BB1_08" class="bgscroll"> BB1_08</a></br>
  
−
<div>
+
<div id="BB1_01" class="modalDialog ">
−
  <header>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <h2>July 2017</h2>
+
<img src="https://static.igem.org/mediawiki/2017/5/5d/T--BOKU-Vienna--BB101.png" style="width:100%;height:auto;"/>
−
  </header>
+
</div></div>
−
        <div>
+
<div id="BB1_02" class="modalDialog ">
−
<table>
+
<div><a href="#close" title="Close" class="close">X</a>
−
  <tr>
+
<img src="https://static.igem.org/mediawiki/2017/1/18/T--BOKU-Vienna--BB102.png" style="width:100%;height:auto;"/>
−
    <th>S</th>
+
</div></div>
−
    <th>M</th>
+
<div id="BB1_03" class="modalDialog ">
−
    <th>T</th>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <th>W</th>
+
<img src="https://static.igem.org/mediawiki/2017/c/c8/T--BOKU-Vienna--BB103.png" style="width:100%;height:auto;"/>
−
    <th>Th</th>
+
</div></div>
−
    <th>F</th>
+
<div id="BB1_04" class="modalDialog ">
−
    <th>S</th>
+
<div><a href="#close" title="Close" class="close">X</a>
−
  </tr>
+
<img src="https://static.igem.org/mediawiki/2017/a/a9/T--BOKU-Vienna--BB104.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB1_05" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/6/6f/T--BOKU-Vienna--BB105.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB1_06" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/a7/T--BOKU-Vienna--BB106.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB1_07" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/c/c3/T--BOKU-Vienna--BB107.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB1_08" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/b9/T--BOKU-Vienna--BB108.png" style="width:100%;height:auto;"/>
 +
</div></div>  
 +
  </p></div>
  
−
  <tr>
+
   
−
    <td></td>
+
<a href="#week2" id="Week 2" class="arrows" onclick="expand_collapse('2');"><pre><font size="+2"><b>Week 2</b></font> (10/07-14/07)</pre></a><span style="font-size:150%;"><b>  </b></span>
−
    <td></td>
+
<div id="2" class="yolo" style="display:none;">
−
    <td></td>
+
<p>
−
    <td></td>
+
<br>• PCR: #1, #2, #3, #4, #6, #15, #18, #20, #23, #24, #25, #26</br>
−
    <td></td>
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
−
    <td></td>
+
<br><br>• Golden Gate Assembly of <a href="#BB1_09" class="bgscroll"> BB1_09</a>, <a href="#BB1_10" class="bgscroll"> BB1_10</a>, <a href="#BB1_11" class="bgscroll"> BB1_11</a>, <a href="#BB1_12" class="bgscroll"> BB1_12</a>, <a href="#BB1_13" class="bgscroll"> BB1_13</a>, BB2_01 (unsuccessful), <a href="#BB2_02" class="bgscroll"> BB2_02</a>, <a href="#BB2_03" class="bgscroll"> BB2_03</a>, <a href="#BB2_04" class="bgscroll"> BB2_04</a>, BB2_05 (unsuccessful)</br>
−
    <td>1</td>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 +
<br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
 +
<br>• Transformation of HSM174 + pSIM5 (unsuccessful)</br>
 +
<br>• Yeast S288C gDNA extraction </br>
  
−
  </tr>
+
<br>• Interlab Challenge 2017: Performance of the measurements. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site.</br>
−
  <tr>
+
−
    <td>2</td>
+
−
    <td><a href="#703">3</a></td>
+
−
    <td><a href="#704">4</a></td>
+
−
    <td><a href="#705">5</a></td>
+
−
    <td><a href="#706">6</a></td>
+
−
    <td><a href="#707">7</a></td>
+
−
    <td>8</td>
+
−
  </tr>
+
  
−
  <tr>
 
−
    <td>9</td>
 
−
    <td><a href="#710">10</a></td>
 
−
    <td><a href="#711">11</a></td>
 
−
    <td><a href="#712">12</a></td>
 
−
    <td><a href="#713">13</a></td>
 
−
    <td><a href="#714">14</a></td>
 
−
    <td>15</td>
 
−
  </tr>
 
  
−
  <tr>
+
<div id="BB1_09" class="modalDialog ">
−
    <td>16</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <td><a href="#717">17</a></td>
+
<img src="https://static.igem.org/mediawiki/2017/1/1a/T--BOKU-Vienna--BB109.png" style="width:100%;height:auto;"/>
−
    <td><a href="#718">18</a></td>
+
</div></div>
−
    <td><a href="#719">19</a></td>
+
<div id="BB1_10" class="modalDialog ">
−
    <td><a href="#720">20</a></td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <td><a href="#721">21</a></td>
+
<img src="https://static.igem.org/mediawiki/2017/5/55/T--BOKU-Vienna--BB110.png" style="width:100%;height:auto;"/>
−
    <td>22</td>
+
</div></div>
−
  </tr>
+
<div id="BB1_11" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/e/e4/T--BOKU-Vienna--BB111.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB1_12" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/5/59/T--BOKU-Vienna--BB112.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB1_13" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/bf/T--BOKU-Vienna--BB113.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_02" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/bd/T--BOKU-Vienna--BB202.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_03" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/3b/T--BOKU-Vienna--BB203.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_04" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/c/cc/T--BOKU-Vienna--BB204.png" style="width:100%;height:auto;"/>
 +
</div></div> 
 +
</p></div>
  
−
  <tr>
+
<a href="#week3" id="Week 3" class="arrows" onclick="expand_collapse('3');"><pre><font size="+2"><b>Week 3</b></font> (17/07-21/07)</pre></a><span style="font-size:150%;"><b>  </b></span>
−
    <td>23</td>
+
<div id="3" class="yolo" style="display:none;">
−
    <td><a href="#724">24</a></td>
+
<p>
−
    <td><a href="#725">25</a></td>
+
<br>• PCR: #27, #28</br>
−
    <td><a href="#726">26</a></td>
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
−
    <td><a href="#727">27</a></td>
+
<br><br>• Golden Gate Assembly of <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>, BB2_01 (unsuccessful, even with new BBa_23101 promoter), BB2_05 (unsuccessful), <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a>, <a href="#BB2_14" class="bgscroll"> BB2_14</a>, <a href="#BB2_15" class="bgscroll"> BB2_15</a>, <a href="#BB2_16" class="bgscroll"> BB2_16</a>, C-PPP_01 (unsuccessful)</br>
−
    <td><a href="#728">28</a></td>  
+
GG-products were transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
−
    <td>29</td>
+
<br>• Sent for Sequencing: <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>,  <a href="#BB2_01" class="bgscroll"> BB2_01</a>, <a href="#BB2_05" class="bgscroll"> BB2_05</a>, <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a></br>
−
  </tr>
+
  
−
  <tr>
 
−
    <td>30</td>
 
−
    <td><a href="#731">31</a></td>
 
−
    <td></td>
 
−
    <td></td>
 
−
    <td></td>
 
−
    <td></td>
 
−
    <td></td>
 
−
  </tr>
 
  
−
</table>
+
<div id="BB1_14" class="modalDialog ">
−
</div>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</div>
+
<img src="https://static.igem.org/mediawiki/2017/e/e1/T--BOKU-Vienna--BB114.png" style="width:100%;height:auto;"/>
−
</div>
+
</div></div>
−
</div>
+
<div id="BB1_15" class="modalDialog ">
−
<div id="mydiv">
+
<div><a href="#close" title="Close" class="close">X</a>
−
<a class="anchor" name="703"></a>
+
<img src="https://static.igem.org/mediawiki/2017/2/29/T--BOKU-Vienna--BB115.png" style="width:100%;height:auto;"/>
−
  <h4>Monday, July 3rd.</h4>
+
</div></div>
−
  <ul class="mylist">
+
<div id="BB2_01" class="modalDialog ">
−
    <li>Team discussion and overview:</li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
      <ul>
+
<img src="https://static.igem.org/mediawiki/2017/b/ba/T--BOKU-Vienna--BB201.png" style="width:100%;height:auto;"/>
−
        <li>Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian</li>
+
</div></div>
−
      </ul>
+
<div id="BB2_05" class="modalDialog ">
−
    <li>Planning of the procedures including primer design</li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li><a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbmedium1" target="_blank">Preparing the growth media and autoclaving</a></li>
+
<img src="https://static.igem.org/mediawiki/2017/6/6a/T--BOKU-Vienna--BB205.png" style="width:100%;height:auto;"/>
−
      <ul><li>LB medium preparation (4L): Mina, Michael</li></ul>
+
</div></div>
−
      <ul><li>Autoclaving: Mina, Rebecca</li></ul>
+
<div id="BB2_06" class="modalDialog ">
−
  </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</div>
+
<img src="https://static.igem.org/mediawiki/2017/9/95/T--BOKU-Vienna--BB206.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_07" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/0/0d/T--BOKU-Vienna--BB207.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_08" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/d/df/T--BOKU-Vienna--BB208.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_09" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/6/63/T--BOKU-Vienna--BB209.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_10" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/9/9a/T--BOKU-Vienna--BB210.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_12" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/0/03/T--BOKU-Vienna--BB212.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_14" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/4/4e/T--BOKU-Vienna--BB214.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_15" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/1/1c/T--BOKU-Vienna--BB215.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_16" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/5/52/T--BOKU-Vienna--BB216.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
  
−
<p></p>
+
<a href="#week4" id="Week 4" class="arrows" onclick="expand_collapse('4');"><pre><font size="+2"><b>Week 4</b></font> (24/07-28/07)</pre></a><span style="font-size:150%;"><b> </b></span>
−
<p></p>
+
<div id="4" class="yolo" style="display:none;">
−
<div id="mydiv">
+
<p>
−
<a name="704" class="anchor"></a>
+
<br>• PCR: #9, #10, #16, #29, #30, #31, #32, #33, #34, #35, #36, #41, #42, #43</br>
−
  <h4>Tuesday, July 4th.</h4>
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
−
<ul class="mylist">
+
<br><br>• Golden Gate Assembly of <a href="#BB1_16" class="bgscroll"> BB1_16</a>, <a href="#BB1_17" class="bgscroll"> BB1_17</a>, <a href="#BB1_18" class="bgscroll"> BB1_18</a>, <a href="#BB1_20" class="bgscroll"> BB1_20</a>, <a href="#BB1_21" class="bgscroll"> BB1_21</a>,  <a href="#BB1_22" class="bgscroll"> BB1_22</a>, <a href="#BB1_23" class="bgscroll"> BB1_23</a>, <a href="#BB1_24" class="bgscroll"> BB1_24</a>, <a href="#BB1_25" class="bgscroll"> BB1_25</a>, <a href="#BB2_17" class="bgscroll"> BB2_17</a>, <a href="#BB2_23" class="bgscroll"> BB2_23</a>, <a href="#BB2_24" class="bgscroll"> BB2_24</a>, BB3_01 (unsuccessful), <a href="#BB3_02" class="bgscroll"> BB3_02</a>, <a href="#BB3_04" class="bgscroll"> BB3_04</a>, <a href="#BB3_05" class="bgscroll"> BB3_05</a>, <a href="#BB3_06" class="bgscroll"> BB3_06</a> (doubtful), <a href="#BB3_08" class="bgscroll"> BB3_08</a></br>
−
    <li>Planning and fundraising:</li>
+
−
      <ul>
+
−
        <li>Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian</li>
+
−
      </ul>
+
−
    <li>Preparation of growth media:</li>
+
−
      <ul><li>20 plates <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbmedium1" target="_blank">LB medium</a> + chloramphenicol: Mina, Lisa, Mathias</li></ul>
+
−
      <ul><li>2 flasks <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbagar" target="_blank">LB agar</a>: Mina, Lisa, Mathias</li></ul>
+
−
    <li>Making cells from straind DH10B chemically competent</li>
+
−
</ul>
+
−
</div>
+
  
−
<p></p>
 
−
<p></p>
 
−
<div id="mydiv">
 
−
<a name="705" class="anchor"></a>
 
−
  <h4>Wednesday, July 5th.</h4>
 
−
  <ul class="mylist">
 
−
    <li>Planning and fundraising:</li>
 
−
      <ul>
 
−
        <li>Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian</li>
 
−
      </ul>
 
−
    <li>Aliquoting 1mL LB into Eppendorf tubes</li>
 
−
        <ul><li>Lisa, Josef</li></ul>
 
−
    <li>Transferring Backbones onto agar plates:</li>
 
−
        <ul><li>Julian</li></ul>
 
−
    <li>Interlab challenge - transformation: According to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#transformation" target="_blank">protocol</a>:</li>
 
−
        <ul>Lisa, Mathias, Michael, Mina, Rebecca</ul>
 
−
        <ul><li>8 transformations - from <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#interlab" target="_blank">Kit Plate 6 InterLab</a></li></ul>
 
−
        <ul><li>8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)</li></ul>
 
−
        <ul><li>stored at 35°C</li></ul>
 
−
  </ul>
 
−
</div>
 
  
−
<p></p>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
−
<p></p>
+
<br>• Yeast S266C were made electro-competent.</br>
−
<div id="mydiv">
+
<br>• Sent for Sequencing: <a href="#BB1_16" class="bgscroll"> BB1_16</a>, <a href="#BB1_17" class="bgscroll"> BB1_17</a>, <a href="#BB1_18" class="bgscroll"> BB1_18</a>, <a href="#BB3_02" class="bgscroll"> BB3_02</a>, <a href="#BB3_05" class="bgscroll"> BB3_05</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_01</a></br>
−
<a name="706" class="anchor"></a>
+
−
  <h4>Thursday, July 6th.</h4>
+
−
  <ul class="mylist">
+
−
    <li>Team discussion, planning and fundraising</li>
+
−
    <li>Counting colonies of Transformants for Determination of Transformation Efficiency of our DH10B cells:</li>
+
−
      <ul><li>Colonies 200µL: 77, 20µL: 26, 100µL: 43</li></ul>
+
−
      <img src="https://static.igem.org/mediawiki/2017/1/17/T--BOKU-Vienna--Notebook_countedplate1.jpg" alt="Transformants on plate" height="200">
+
−
      <img src="https://static.igem.org/mediawiki/2017/f/f6/T--BOKU-Vienna--Notebook_countedplate2.jpg" alt="Transformants on plate" height="200">
+
−
      <img src="https://static.igem.org/mediawiki/2017/2/2c/T--BOKU-Vienna--Notebook_countedplate3.jpg" alt="Transformants on plate" height="200">
+
−
    <li>Miniprep of 8 plasmids for Golden Gate Standard - according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#miniprep" target="_blank">protocol</a></li>
+
−
      <ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul>
+
−
      <ul><li>Dilution of plasmids for Golden Gate: Lisa</li></ul>
+
−
  </ul>
+
  
−
<h5 id="dilution">Dilution sheet for Golden Gate Assembly</h5>
+
<div id="BB1_16" class="modalDialog ">
−
<p></p>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<div>
+
<img src="https://static.igem.org/mediawiki/2017/d/d9/T--BOKU-Vienna--BB116.png" style="width:100%;height:auto;"/>
−
<table class="table1" >
+
</div></div>
−
<thead>
+
<div id="BB1_17" class="modalDialog ">
−
<tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<th>Sample</th>
+
<img src="https://static.igem.org/mediawiki/2017/e/e1/T--BOKU-Vienna--BB117.png" style="width:100%;height:auto;"/>
−
                <th>Volume of AD - RO Water [µL]</th>
+
</div></div>
−
<th>Volume of plasmid/fragment [µL]</th>
+
<div id="BB1_18" class="modalDialog ">
−
</tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</thead>
+
<img src="https://static.igem.org/mediawiki/2017/3/3c/T--BOKU-Vienna--BB118.png" style="width:100%;height:auto;"/>
−
<tbody>
+
</div></div>
−
<tr>
+
<div id="BB1_20" class="modalDialog ">
−
<td>BB1-C-23-Spy-Cas9(NLS)-49</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>71,6</td>
+
<img src="https://static.igem.org/mediawiki/2017/a/a7/T--BOKU-Vienna--BB120.png" style="width:100%;height:auto;"/>
−
                <td>28,4</td>
+
</div></div>
−
</tr>
+
<div id="BB1_21" class="modalDialog ">
−
<tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>BB1-T-34-sce-cyc-50</td>
+
<img src="https://static.igem.org/mediawiki/2017/5/58/T--BOKU-Vienna--BB121.png" style="width:100%;height:auto;"/>
−
<td>83,0</td>
+
</div></div>
−
                <td>17,0</td>
+
<div id="BB1_22" class="modalDialog ">
−
</tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
        <tr>
+
<img src="https://static.igem.org/mediawiki/2017/f/f4/T--BOKU-Vienna--BB122.png" style="width:100%;height:auto;"/>
−
<td>BB1-P-12-syr-pNS-J23101-25</td>
+
</div></div>
−
<td>85,1</td>
+
<div id="BB1_23" class="modalDialog ">
−
                <td>14,9</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</tr>
+
<img src="https://static.igem.org/mediawiki/2017/d/d3/T--BOKU-Vienna--BB123.png" style="width:100%;height:auto;"/>
−
        <tr>
+
</div></div>
−
<td>BB1-P-12-sce-tdh3-45</td>
+
<div id="BB1_24" class="modalDialog ">
−
<td>82,7</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
                <td>17,3</td>
+
<img src="https://static.igem.org/mediawiki/2017/b/b6/T--BOKU-Vienna--BB124.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
        <tr>
+
<div id="BB1_25" class="modalDialog ">
−
<td>BB1-T-34-eco-B1001-29</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>80,4</td>
+
<img src="https://static.igem.org/mediawiki/2017/5/51/T--BOKU-Vienna--BB125.png" style="width:100%;height:auto;"/>
−
                <td>19,6</td>
+
</div></div>
−
</tr>
+
<div id="BB2_17" class="modalDialog ">
−
        <tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>BB1-P-12-syn-pm-J23105-23</td>
+
<img src="https://static.igem.org/mediawiki/2017/f/fd/T--BOKU-Vienna--BB217.png" style="width:100%;height:auto;"/>
−
<td>83,9</td>
+
</div></div>
−
                <td>16,1</td>
+
<div id="BB2_23" class="modalDialog ">
−
</tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
        <tr>
+
<img src="https://static.igem.org/mediawiki/2017/b/be/T--BOKU-Vienna--BB223.png" style="width:100%;height:auto;"/>
−
<td>BB1-C-23-syn-GFP-30</td>
+
</div></div>
−
<td>82,9</td>
+
<div id="BB2_24" class="modalDialog ">
−
                <td>17,1</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</tr>
+
<img src="https://static.igem.org/mediawiki/2017/a/a4/T--BOKU-Vienna--BB224.png" style="width:100%;height:auto;"/>
−
        <tr>
+
</div></div>
−
<td>BB1-P-12-sce-tef1-46</td>
+
<div id="BB3_02" class="modalDialog ">
−
<td>78,0</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
                <td>22,0</td>
+
<img src="https://static.igem.org/mediawiki/2017/4/46/T--BOKU-Vienna--BB302.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
</tbody>
+
<div id="BB3_04" class="modalDialog ">
−
</table>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</div>
+
<img src="https://static.igem.org/mediawiki/2017/4/4a/T--BOKU-Vienna--BB304.png" style="width:100%;height:auto;"/>
−
</div>
+
</div></div>
−
<p></p>
+
<div id="BB3_05" class="modalDialog ">
−
<p></p>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<div id="mydiv">
+
<img src="https://static.igem.org/mediawiki/2017/6/62/T--BOKU-Vienna--BB305.png" style="width:100%;height:auto;"/>
−
<a name="707" class="anchor"></a>
+
</div></div>
−
  <h4>Friday, July 7th.</h4>
+
<div id="BB3_06" class="modalDialog ">
−
<ul class="mylist">
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>Team discussion, Planning and Fundraising</li>
+
<img src="https://static.igem.org/mediawiki/2017/d/da/T--BOKU-Vienna--BB306.png" style="width:100%;height:auto;"/>
−
    <li><a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#interlab" target="_blank">Interlab Challenge</a>: Fluorescein fluorescence standard curve</li>
+
</div></div>
−
    <ul><li>Christina</li></ul>
+
<div id="BB3_08" class="modalDialog ">
−
  </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</div>
+
<img src="https://static.igem.org/mediawiki/2017/1/1c/T--BOKU-Vienna--BB308.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_01" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/4/40/T--BOKU-Vienna--CPPP01.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
  
−
<div id="mydiv">
 
−
<a name="710" class="anchor"></a>
 
−
  <h4>Monday, July 10th.</h4>
 
−
  <ul class="mylist">
 
−
    <li>InterLab Study:</li>
 
−
      <ul>
 
−
        <li>Christina, Felicia</li>
 
−
      </ul>
 
−
    <li>Dilution of several primers 1:10 (AD-RO Water µL45 , Primer µL5)</li>
 
−
      <ul><li>Magdalena</li>
 
−
      </ul>
 
−
    <li>PCR Gel Preparation</li>
 
−
      <ul><li>Mathias</li>
 
−
      </ul>
 
−
  </ul>
 
−
</div>
 
  
−
<p></p>
+
<a href="#week5" id="Week 5" class="arrows" onclick="expand_collapse('5');"><pre><font size="+2"><b>Week 5</b></font> (31/07-04/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
−
<p></p>
+
<div id="5" class="yolo" style="display:none;">
 +
<p>
 +
<br>• PCR: #41, #42, #45, #52, #53, #56, #57</br>
 +
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 +
<br><br>• Golden Gate Assembly of <a href="#BB1_19" class="bgscroll"> BB1_19</a>, <a href="#BB2_19" class="bgscroll"> BB2_19</a>, <a href="#BB2_25" class="bgscroll"> BB2_25</a>, <a href="#BB2_26" class="bgscroll"> BB2_26</a>, <a href="#BB2_27" class="bgscroll"> BB2_27</a>, <a href="#BB2_28" class="bgscroll"> BB2_28</a>, <a href="#BB2_29" class="bgscroll"> BB2_29</a>, <a href="#BB2_30" class="bgscroll"> BB2_30</a>, <a href="#BB2_31" class="bgscroll"> BB2_31</a>, <a href="#BB2_32" class="bgscroll"> BB2_32</a>, <a href="#BB2_33" class="bgscroll"> BB2_33</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, BB3_01 (unsuccessful), <a href="#BB3_02" class="bgscroll"> BB3_02</a>, BB3_03 (unsuccessful), <a href="#BB3_06" class="bgscroll"> BB3_06</a>, <a href="#BB3_10" class="bgscroll"> BB3_10</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#BB3_15" class="bgscroll"> BB3_15</a>, <a href="#BB3_24" class="bgscroll"> BB3_24</a></br>
 +
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 +
<br>• DH10B <i>E. coli</i> strain was made chemically competent.</br>
 +
<br>• Yeast cells were transformed with the Cas9 plasmid.</br>
 +
<br>• Sent for Sequencing: <a href="#BB1_19" class="bgscroll"> BB1_19</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_02</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a></br>
  
−
<div id="mydiv">
+
<div id="BB1_19" class="modalDialog ">
−
<a name="711" class="anchor"></a>
+
<div><a href="#close" title="Close" class="close">X</a>
−
  <h4>Tuesday, July 11th.</h4>
+
<img src="https://static.igem.org/mediawiki/2017/d/dd/T--BOKU-Vienna--BB119.png" style="width:100%;height:auto;"/>
−
  <ul class="mylist">
+
</div></div>
−
    <li>Planning & Fundraising</li>
+
<div id="BB2_19" class="modalDialog ">
−
    <li>Golden Gate Assembly:</li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
      <ul><li>Flpe (BB1), GFP (BB2) → Transformation (according to protocol) → Plating onto Agar (After Transformation): Selection with Ampicillin (bb1),  Selection with Kanamycin(bb2), stored at 37°C over night
+
<img src="https://static.igem.org/mediawiki/2017/f/f1/T--BOKU-Vienna--BB219.png" style="width:100%;height:auto;"/>
−
      </li></ul>
+
</div></div>
−
    <li>Dilution of several primer</li>
+
<div id="BB2_25" class="modalDialog ">
−
      <ul><table class="table1">
+
<div><a href="#close" title="Close" class="close">X</a>
−
<thead>
+
<img src="https://static.igem.org/mediawiki/2017/9/97/T--BOKU-Vienna--BB225.png" style="width:100%;height:auto;"/>
−
<tr>
+
</div></div>
−
<th>Abbreviation</th>
+
<div id="BB2_26" class="modalDialog ">
−
                <th>Primer</th>
+
<div><a href="#close" title="Close" class="close">X</a>
−
</tr>
+
<img src="https://static.igem.org/mediawiki/2017/2/25/T--BOKU-Vienna--BB226.png" style="width:100%;height:auto;"/>
−
</thead>
+
</div></div>
−
<tbody>
+
<div id="BB2_27" class="modalDialog ">
−
<tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>1_fw</td>
+
<img src="https://static.igem.org/mediawiki/2017/0/05/T--BOKU-Vienna--BB227.png" style="width:100%;height:auto;"/>
−
<td>pBAD_fw_FS_1</td>
+
</div></div>
−
             
+
<div id="BB2_28" class="modalDialog ">
−
</tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<tr>
+
<img src="https://static.igem.org/mediawiki/2017/0/04/T--BOKU-Vienna--BB228.png" style="width:100%;height:auto;"/>
−
<td>2_fw</td>
+
</div></div>
−
<td>RT_fw_FS2_BpiI</td>
+
<div id="BB2_29" class="modalDialog ">
−
           
+
<div><a href="#close" title="Close" class="close">X</a>
−
</tr>
+
<img src="https://static.igem.org/mediawiki/2017/c/c0/T--BOKU-Vienna--BB229.png" style="width:100%;height:auto;"/>
−
        <tr>
+
</div></div>
−
<td>2_rev</td>
+
<div id="BB2_30" class="modalDialog ">
−
<td>RT_rev_RBS_FS_C_BpiI</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
             
+
<img src="https://static.igem.org/mediawiki/2017/d/d8/T--BOKU-Vienna--BB230.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
        <tr>
+
<div id="BB2_31" class="modalDialog ">
−
<td>3_fw</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>FLPe_fw_FS_C_BpiI</td>
+
<img src="https://static.igem.org/mediawiki/2017/a/ad/T--BOKU-Vienna--BB231.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
        <tr>
+
<div id="BB2_32" class="modalDialog ">
−
<td>3_rev</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>FLPe_rev_FS_3_BpiI</td>
+
<img src="https://static.igem.org/mediawiki/2017/3/3a/T--BOKU-Vienna--BB232.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
        <tr>
+
<div id="BB2_33" class="modalDialog ">
−
<td>4_fw</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>RepA101ts_fw_FS_23_BsaI</td>
+
<img src="https://static.igem.org/mediawiki/2017/b/bd/T--BOKU-Vienna--BB233.png" style="width:100%;height:auto;"/>
−
           
+
</div></div>
−
</tr>
+
<div id="BB2_34" class="modalDialog ">
−
        <tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>4_rev</td>
+
<img src="https://static.igem.org/mediawiki/2017/3/34/T--BOKU-Vienna--BB234.png" style="width:100%;height:auto;"/>
−
<td>RepA101ts_rev_FS_3_BsaI</td>
+
</div></div>
−
</tr>
+
<div id="BB3_02" class="modalDialog ">
−
        <tr>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>5_fw</td>
+
<img src="https://static.igem.org/mediawiki/2017/4/46/T--BOKU-Vienna--BB302.png" style="width:100%;height:auto;"/>
−
<td>AmpR_Intron_Fw_ohne_FRT_FS_2_BsaI</td>
+
</div></div>
−
</tr>
+
<div id="BB3_06" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/d/da/T--BOKU-Vienna--BB306.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_10" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/6/65/T--BOKU-Vienna--BB310.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_12" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/3c/T--BOKU-Vienna--BB312.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_13" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/7/72/T--BOKU-Vienna--BB313.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_14" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/b2/T--BOKU-Vienna--BB314.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_15" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/2c/T--BOKU-Vienna--BB315.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_24" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/c/c1/T--BOKU-Vienna--BB324.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_01" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/4/40/T--BOKU-Vienna--CPPP01.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
  
−
        <tr>
+
<a href="#week6" id="Week 6" class="arrows" onclick="expand_collapse('6');"><pre><font size="+2"><b>Week 6</b></font> (07/08-11/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
−
<td>5_rev</td>
+
<div id="6" class="yolo" style="display:none;">
−
<td>AmpR_Intron_rev_ohne_FRT_FS_3_BsaI</td>
+
<p>
−
</tr>
+
<br>• Golden Gate Assembly of <a href="#BB2_36" class="bgscroll"> BB2_36</a>, <a href="#BB3_07" class="bgscroll"> BB3_07</a>, <a href="#BB3_10" class="bgscroll"> BB3_10</a>, <a href="#BB3_11" class="bgscroll"> BB3_11</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_18" class="bgscroll"> BB3_18</a>, <a href="#BB3_19" class="bgscroll"> BB3_19</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_21" class="bgscroll"> BB3_21</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, <a href="#BB3_23" class="bgscroll"> BB3_23</a>, <a href="#BB3_24" class="bgscroll"> BB3_24</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a></br>
−
          <tr>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected  by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
−
<td>6_fw</td>
+
<br>• A temperature-sensitivity test was performed for cells with pSIM5, a temperature sensitive plasmid. An overnight culture was incubated at 37°C and a control was incubated at 28°C. The overnight culture at 37°C was tested as positive and lost the plasmid, while the control maintained its plasmid.</br>
−
<td>KanR_CripsR_fw_FS_B_BsaI</td>
+
<br>• Two clones of yeast cells carrying the Cas9 plasmid were made competent for the next transformation.</br>
−
           
+
<br>• Sent for Sequencing: <a href="#BB2_19" class="bgscroll"> BB2_19</a>, <a href="#BB2_21" class="bgscroll"> BB2_21</a>, <a href="#BB2_31" class="bgscroll"> BB2_31</a>, <a href="#BB2_32" class="bgscroll"> BB2_32</a>, <a href="#BB2_33" class="bgscroll"> BB2_33</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, <a href="#BB3_01" class="bgscroll"> BB3_01</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_02</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a></br>
−
</tr>
+
 
−
        <tr>
+
<div id="BB2_19" class="modalDialog ">
−
<td>6_rev</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>KanR_CrispR_rev_Fs_E_BsaI</td>
+
<img src="https://static.igem.org/mediawiki/2017/f/f1/T--BOKU-Vienna--BB219.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
        <tr>
+
<div id="BB2_21" class="modalDialog ">
−
<td>7_fw</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>AmpR_Intron_Fw_FRT_FS_2_BsaI</td>
+
<img src="https://static.igem.org/mediawiki/2017/c/ce/T--BOKU-Vienna--BB221.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
        <tr>
+
<div id="BB2_31" class="modalDialog ">
−
<td>7_rev</td>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<td>AmpR_Intron_rev_FRT_FS_3_BsaI</td>
+
<img src="https://static.igem.org/mediawiki/2017/a/ad/T--BOKU-Vienna--BB231.png" style="width:100%;height:auto;"/>
−
</tr>
+
</div></div>
−
</tbody>
+
<div id="BB2_32" class="modalDialog ">
−
</table>
+
<div><a href="#close" title="Close" class="close">X</a>
−
      </ul>
+
<img src="https://static.igem.org/mediawiki/2017/3/3a/T--BOKU-Vienna--BB232.png" style="width:100%;height:auto;"/>
−
  <li>PCR plasmid fragments</li>
+
</div></div>
−
      <ul><li>Josef</li>
+
<div id="BB2_33" class="modalDialog ">
−
      </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
        <ul><li>fragment 1: GFP+Primer binding side, fragment 2: GFP + ½ FRT sequence, fragment 3: 1 random sequence
+
<img src="https://static.igem.org/mediawiki/2017/b/bd/T--BOKU-Vienna--BB233.png" style="width:100%;height:auto;"/>
−
      </li></ul>
+
</div></div>
−
  <li>Gel electrophoresis</li>
+
<div id="BB2_34" class="modalDialog ">
−
  </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/34/T--BOKU-Vienna--BB234.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_36" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/be/T--BOKU-Vienna--BB236.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_07" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/2d/T--BOKU-Vienna--BB307.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_10" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/6/65/T--BOKU-Vienna--BB310.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_11" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/39/T--BOKU-Vienna--BB311.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_13" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/7/72/T--BOKU-Vienna--BB313.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_14" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/b2/T--BOKU-Vienna--BB314.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_17" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/36/T--BOKU-Vienna--BB317.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_18" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/f/f7/T--BOKU-Vienna--BB318.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_19" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/5/52/T--BOKU-Vienna--BB319.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_21" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/af/T--BOKU-Vienna--BB321.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_23" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/f/ff/T--BOKU-Vienna--BB323.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_24" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/c/c1/T--BOKU-Vienna--BB324.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_01" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/4/40/T--BOKU-Vienna--CPPP01.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_04" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/a3/T--BOKU-Vienna--CPPP04.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_20" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/24/T--BOKU-Vienna--BB320.png" style="width:100%;height:auto;"/>
 
</div>
 
</div>
−
 
−
<p></p>
 
−
<p></p>
 
−
<div id="mydiv">
 
−
<a name="712" class="anchor"></a>
 
−
  <h4>Wednesday, July 12th.</h4>
 
−
  <ul class="mylist">
 
−
    <li>Gel PCR for Kanamycin casette</li>
 
−
      <ul>
 
−
        <li>Mina, Magda</li>
 
−
      </ul>
 
−
<li>Restriction digest</li>
 
−
<li>Gel/PCR DNA Extraction </li>
 
−
<li>NEB Golden Gate Assembly Mix</li>
 
−
 
 
−
  <li>Miniprep</li>
 
−
  <ul><li>Lisa</li></ul>
 
−
  <li>InterLab Study</li>
 
−
    <ul>
 
−
        <li>Felicia, Christina</li>
 
−
    </ul>
 
−
<li>PCR and gel electrophoresis</li>
 
−
    <ul>
 
−
        <li>Michael</li>
 
−
    </ul>
 
−
  </ul>
 
 
</div>
 
</div>
 +
<div id="BB3_22" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/21/T--BOKU-Vienna--BB322.png" style="width:100%;height:auto;"/>
 +
</div>
 +
</div>
 +
</p></div>
 +
               
 +
<a href="#week7" id="Week 7" class="arrows" onclick="expand_collapse('7');"><pre><font size="+2"><b>Week 7</b></font> (14/08-18/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
 +
<div id="7" class="yolo" style="display:none;">
 +
<p>
 +
<br>• Golden Gate Assembly of <a href="#BB1_26" class="bgscroll"> BB1_26</a>, <a href="#BB2_23" class="bgscroll"> BB2_23</a>, BB3_02 (unsuccessful), <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_16" class="bgscroll"> BB3_16</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, BB3_28 (unsuccessful), <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a></br>
 +
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 +
<br>• Overnight cultures of <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a> were tested for temperature sensitivity.</br>
 +
<br>• Sent for Sequencing: <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a></br>
  
−
<p></p>
+
<div id="BB1_26" class="modalDialog ">
−
<p></p>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<div id="mydiv">
+
<img src="https://static.igem.org/mediawiki/2017/f/f2/T--BOKU-Vienna--BB126.png" style="width:100%;height:auto;"/>
−
<a name="713" class="anchor"></a>
+
</div></div>
−
  <h4>Thursday, July 13th.</h4>
+
<div id="BB2_23" class="modalDialog ">
−
  <ul class="mylist">
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>Miniprep: Flpe + GFP BB2, control digest</li>
+
<img src="https://static.igem.org/mediawiki/2017/b/be/T--BOKU-Vienna--BB223.png" style="width:100%;height:auto;"/>
−
      <ul>
+
</div></div>
−
        <li>Lisa, Mina, Valentin</li>
+
<div id="BB3_12" class="modalDialog ">
−
      </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>Minis onto LB Kanamycin medium</li>
+
<img src="https://static.igem.org/mediawiki/2017/3/3c/T--BOKU-Vienna--BB312.png" style="width:100%;height:auto;"/>
−
        <ul><li>Rebecca</li></ul>
+
</div></div>
−
   
+
<div id="BB3_16" class="modalDialog ">
−
    <li>Control digest - GFP</li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
      <ul><li>Mina, Lisa</li></ul>
+
<img src="https://static.igem.org/mediawiki/2017/8/8a/T--BOKU-Vienna--BB316.png" style="width:100%;height:auto;"/>
−
     
+
</div></div>
−
    <li>Gel electrophoresis</li>
+
<div id="BB3_17" class="modalDialog ">
−
      <ul><li>Felicia, Mathias</li></ul>
+
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/36/T--BOKU-Vienna--BB317.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_20" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/24/T--BOKU-Vienna--BB320.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_22" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/21/T--BOKU-Vienna--BB322.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_28" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/e/e5/T--BOKU-Vienna--BB328.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_04" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/a3/T--BOKU-Vienna--CPPP04.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
  
−
    <li>Golden Gate Assembly:</li>
+
<a href="#week8" id="Week 8" class="arrows" onclick="expand_collapse('8');"><pre><font size="+2"><b>Week 8</b></font> (21/08-25/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
−
      <ul><li>Joseph, Felicia, Mina, Christina, Rebecca</li></ul>
+
<div id="8" class="yolo" style="display:none;">
−
  </ul>
+
<p>
−
</div>
+
<br>• PCR: #71, #72</br>
 +
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to protocol.
 +
<br><br>• Golden Gate Assembly of <a href="#BB1_27" class="bgscroll"> BB1_27</a>, BB3_01 (unsuccessful), <a href="#BB3_03" class="bgscroll"> BB3_03</a>, <a href="#BB3_30" class="bgscroll"> BB3_30</a>, <a href="#BB3_31" class="bgscroll"> BB3_31</a>, <a href="#BB3_32" class="bgscroll"> BB3_32</a>, <a href="#BB3_33" class="bgscroll"> BB3_33</a></br>
 +
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 +
<br>• Transformation of <a href="#BB3_32" class="bgscroll"> BB3_32</a> and <a href="#BB3_31" class="bgscroll"> BB3_31</a> into yeast cells</br>
 +
<br>• <a href="#BB3_31" class="bgscroll"> BB3_31</a> and <a href="#BB3_32" class="bgscroll"> BB3_32</a> cultivated in YP+G418: for testing the GFP expression, measurement in a plate reader.
  
  
 +
<div id="BB1_27" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/b8/T--BOKU-Vienna--BB127.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_03" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/39/T--BOKU-Vienna--BB303.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_30" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/9/93/T--BOKU-Vienna--BB330.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_31" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/6/69/T--BOKU-Vienna--BB331.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_32" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/0/0a/T--BOKU-Vienna--BB332.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_33" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/1/1b/T--BOKU-Vienna--BB333.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
  
−
<p></p>
+
<a href="#week9" id="Week 9" class="arrows" onclick="expand_collapse('9');"><pre><font size="+2"><b>Week 9</b></font> (28/08-01/09)</pre></a><span style="font-size:150%;"><b> </b></span>
−
<p></p>
+
<div id="9" class="yolo" style="display:none;">
−
<div id="mydiv">
+
<p>
−
<a name="714" class="anchor"></a>
+
<br>Improving BBa_E0040: PCRs for removing the illegal BsaI restricion site. Golden Gate Assembly of the PCRs and cloning into the pSB1C3. For more information please visit:
−
  <h4>Friday, July 14th.</h4>
+
<a href="https://2017.igem.org/Team:BOKU-Vienna/Improve" target="_blank">Improve</a>
−
  <ul class="mylist">
+
</br>
−
    <li>Transformation from GG-clones in competent cells using LB medium (with Kan or Amp)</li>
+
−
      <ul>
+
−
        <li>Christina, Joseph, Felicia, Melanie, Rebecca </li>
+
−
      </ul>
+
  
−
    <li>Golden Gate Assembly: </li>
+
<br>• Golden Gate Assembly of BB3_01 (unsuccessful), <a href="#C-PPP_07" class="bgscroll">C-PPP_07</a>, <a href="#C-PPP_07" class="bgscroll"> C-PPP_08</a>, <a href="#C-PPP_07" class="bgscroll"> C-PPP_09</a>, <a href="#C-PPP_10" class="bgscroll">C-PPP_10</a>, <a href="#BB3_33" class="bgscroll">BB3_33</a></br>
−
      <ul><li>stored at 37°C</li></ul>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
−
      <ul><li>Joseph, Felicia, Mina, Christina, Rebecca, Lisa, Mathias, Valentin</li></ul>
+
−
   
+
−
    <li>Gel electrophoresis</li>
+
−
      <ul><li>Felicia, Valentin</li></ul>
+
−
    <li>Purifiying DNA fragments from elektrophoresis according to given protocol in the HiYield Gel/PCR DNA Extraction Kit</li>
+
−
        <ul><li>Felicia, Christina, Valentin, Lisa</li></ul>        
+
−
    <li>Concentration measurement of purified DNA</li>
+
−
        <ul><li> Julian</li></ul>
+
−
  </ul>
+
−
</div>
+
  
 +
<br>• Minipreparation for plasmid DNA extraction: <a href="#BB3_12" class="bgscroll">BB3_12</a>, <a href="#BB3_30" class="bgscroll">BB3_30</a>, <a href="#BB3_15" class="bgscroll">BB3_15</a></br>
 +
<br>• Transformation of pSIM9</br>
  
−
<p></p>
+
<div id="BB3_35" class="modalDialog ">
−
<p></p>
+
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/0/06/T--BOKU-Vienna--BB335.png" style="width:100%;height:auto;"/>
 +
</div>
 +
</div>
  
−
<div id="mydiv">
+
<div id="BB3_01" class="modalDialog ">
−
<a name="717" class="anchor"></a>
+
<div><a href="#close" title="Close" class="close">X</a>
−
  <h4>Monday, July 17th.</h4>
+
<img src="https://static.igem.org/mediawiki/2017/4/4f/T--BOKU-Vienna--BB301.png" style="width:100%;height:auto;"/>
−
  <ul class="mylist">
+
</div>
−
    <li>Miniprep</li>
+
</div>
−
      <ul>
+
−
        <li>control digest</li>
+
−
      </ul>
+
−
      <ul>
+
−
        <li>BB1_10, BB1_11, BB1_12, BB2_2, BB2_3, BB2_4, BB2_5: </li>
+
−
      </ul>
+
−
      <ul>
+
−
        <li>Gel electrophoresis</li>
+
−
      </ul>
+
−
      <ul>
+
−
        <li>Mathias, Mina, Magda, Melanie, Lisa, Rebecca</li>
+
−
      </ul>
+
  
−
    <li>Mini: p5IM5 30°C, Kryo</li>
 
−
      <ul><li>Rebecca</li></ul>
 
−
      <ul><li>Control Transformation</li></ul>
 
−
 
−
    <li>PCR</li>
 
−
      <ul><li>Golden Gate: BB2_6 (Rep A, BB2_7: Cas 9, BB2_8: RT, Flpe, BB2_8 → RT, Flpe coli, BB2_1+BB2_5) + Trafo (on LB AMP)
 
−
</li></ul>
 
−
    <li>Purifiying DNA fragments from elektrophoresis according to given protocol in the HiYield Gel/PCR DNA Extraction Kit</li>     
 
−
    <li>Transformation of Golden Gate products:</li>
 
−
        <ul><li>Michi, Julian, Christina, Felicia</li></ul>
 
−
 
 
  
−
<li>Kryo for Sequencing</li>
+
<div id="C-PPP_10" class="modalDialog ">
−
      <ul class="mylist">
+
<div><a href="#close" title="Close" class="close">X</a>
−
        <li>Kryo: tube with Glycerine + sample</li>
+
<img src="https://static.igem.org/mediawiki/2017/9/9f/T--BOKU-Vienna--CPPP10.png" style="width:100%;height:auto;"/>
−
      </ul>
+
</div>
−
  </ul>
+
 
</div>
 
</div>
  
  
−
<p></p>
+
<div id="BB3_33" class="modalDialog ">
−
<p></p>
+
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/1/1b/T--BOKU-Vienna--BB333.png" style="width:100%;height:auto;"/>
 +
</div>
 +
</div>
  
−
<div id="mydiv">
+
<div id="BB3_12" class="modalDialog ">
−
<a name="718" class="anchor"></a>
+
<div><a href="#close" title="Close" class="close">X</a>
−
  <h4>Tuesday, July 18th.</h4>
+
<img src="https://static.igem.org/mediawiki/2017/3/3c/T--BOKU-Vienna--BB312.png" style="width:100%;height:auto;"/>
−
  <ul class="mylist">
+
</div>
−
    <li>Load gel for gel electrophoresis</li>
+
</div>
−
      <ul>
+
−
        <li>Melanie</li>
+
−
      </ul>
+
−
    <li>Media preparation</li>
+
−
        <ul>
+
−
        <li>Melanie, Valentin</li>
+
−
      </ul>
+
−
    <li>Colony PCR (11 colonies)</li>
+
−
      <ul><li>Magdalena, Felicia, Christina, Mina</li></ul>
+
  
−
    <li>Preparation for Sequencing (cryo)</li>
+
<div id="BB3_30" class="modalDialog ">
−
        <ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
        <li>Christina</li>
+
<img src="https://static.igem.org/mediawiki/2017/9/93/T--BOKU-Vienna--BB330.png" style="width:100%;height:auto;"/>
−
      </ul>
+
</div>
−
  <li>more colony PCR</li>
+
</div>
−
        <ul>
+
−
        <li>Felicia, Christina, Valentin, Rebecca, Lisa</li>
+
−
      </ul>
+
  
−
  <li>PCR Reaction with ThermoCycler</li>
+
<div id="BB3_15" class="modalDialog ">
−
  </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/2c/T--BOKU-Vienna--BB315.png" style="width:100%;height:auto;"/>
 +
</div>
 +
</div>
 +
<div id="C-PPP_07" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/2d/T--BOKU-Vienna--CPPP07.png" style="width:100%;height:auto;"/>
 +
</div>
 
</div>
 
</div>
  
−
<p></p>
 
−
<p></p>
 
−
<div id="mydiv">
 
−
<a name="719" class="anchor"></a>
 
−
  <h4>Wednesday, July 19th.</h4>
 
−
  <ul class="mylist">
 
−
    <li>PCR gel elektrophoresis</li>
 
−
      <ul>
 
−
        <li>Melanie, Christina, Felicia, Magdalena</li>
 
−
      </ul>
 
−
    <li>Media preparation (Agar)</li>
 
−
        <ul>
 
−
        <li>Lisa, Rebecca</li>
 
−
      </ul>
 
−
    <li>Miniprep</li>
 
−
      <ul><li>Christina, Felicia, Valentin, Rebecca</li></ul>
 
  
−
    <li>Preparation for Sequencing</li>
 
−
        <ul>
 
−
        <li>Christina, Melanie</li>
 
−
      </ul>
 
−
  <li>Golden Gate</li>
 
−
        <ul>
 
−
        <li>Mathias, Felicia, Lisa, Rebecca</li>
 
−
      </ul>
 
  
−
  <li>Transformation for Golden Gate</li>
+
</p></div>
−
      <ul>
+
−
        <li>Magdalena, Lisa, Mina, Rebecca, Mathias, Julian, Michael</li>
+
−
      </ul>
+
−
  </ul>
+
−
</div>
+
  
  
−
<p></p>
+
<a href="#week10" id="Week 10" class="arrows" onclick="expand_collapse('10');"><pre><font size="+2"><b>Week 10</b></font> (04/09-08/09)</pre></a><span style="font-size:150%;"><b> </b></span>
−
<p></p>
+
<div id="10" class="yolo" style="display:none;">
−
<div id="mydiv">
+
<p>
−
<a name="720" class="anchor"></a>
+
<br>• PCR: #88, #89, #90</br>
−
  <h4>Thursday, July 20th.</h4>
+
−
  <ul class="mylist">
+
−
    <li>Colony PCR:</li>
+
−
      <ul><li>
+
−
      9 samples: BB2_1 A, B, C, D, E, F und BB2_11 A, B, C
+
−
      </li>
+
−
      </ul>
+
−
      <ul>
+
−
        <li>Lisa, Christina, Felicia, Rebecca, Lisa, Mina</li>
+
−
      </ul>
+
−
    <li>Golden Gate repetition (CRISPR: 5alpha, 10B):</li>
+
−
        <ul>
+
−
        <li>Felicia</li>
+
−
      </ul>
+
−
    <li>DH10B transformation of Golden Gate products (5alpha, 10B):</li>
+
−
      <ul><li>Christina, Felicia</li></ul>
+
  
−
    <li>PCR #5</li>
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
−
        <ul>
+
<br><br>• Golden Gate Assembly of  <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_34" class="bgscroll"> BB3_34</a>, <a href="#BB3_35 Speziale" class="bgscroll"> BB3_35 Speziale</a>, <a href="#BB3_35" class="bgscroll"> BB3_35</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
−
        <li>Christina</li>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
−
      </ul>
+
<br>• Lethality-Test of <a href="#C-PPP_10" class="bgscroll"> C-PPP_11</a> and <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
−
  <li>Gel electrophoresis</li>
+
<div id="BB3_34" class="modalDialog ">
−
        <ul><li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
          with Colony PCR products, Golden Gate products, PCR #5
+
<img src="https://static.igem.org/mediawiki/2017/a/a2/T--BOKU-Vienna--BB334.png" style="width:100%;height:auto;"/>
−
        </li></ul>
+
</div></div>
−
        <ul>
+
<div id="BB3_35 Speziale" class="modalDialog ">
−
        <li>Felicia, Christina</li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
      </ul>
+
<img src="https://static.igem.org/mediawiki/2017/e/ee/T--BOKU-Vienna--BB335_speziale.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_35" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/0/06/T--BOKU-Vienna--BB335.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_36" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/b6/T--BOKU-Vienna--BB336.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_37" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/d/d9/T--BOKU-Vienna--BB337.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_38" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/ad/T--BOKU-Vienna--BB338.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_10" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/9/9f/T--BOKU-Vienna--CPPP10.png" style="width:100%;height:auto;"/>
 +
</div></div>
  
−
  <li>DNA purification of PCR #6</li>
+
</p></div>
−
      <ul>
+
−
        <li>Christina</li>
+
−
      </ul>
+
−
  <li>Aliquoting Master Mix</li>
+
−
      <ul>
+
−
        <li>Valentin</li>
+
−
      </ul>
+
−
  <li>Golden Gate (GFP, BB2_16)</li>
+
−
      <ul>
+
−
        <li>Valentin, Mathias, Rebecca</li>
+
−
      </ul>
+
−
  <li>Plating repA colony onto agar</li>
+
−
      <ul>
+
−
        <li>Lisa, Valentin</li>
+
−
      </ul>
+
−
  <li>Transformation</li>
+
−
      <ul>
+
−
        <li>Michael, Julian, Felicia, Christina</li>
+
−
      </ul>
+
−
  </ul>
+
−
</div>
+
  
 +
<a href="#week11" id="Week 11" class="arrows" onclick="expand_collapse('11');"><pre><font size="+2"><b>Week 11</b></font> (11/09-15/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
 +
<div id="11" class="yolo" style="display:none;">
 +
<p>
 +
<br>• Golden Gate Assembly of <a href="#BB3_27" class="bgscroll"> BB3_27</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#BB3_39" class="bgscroll"> BB3_39</a>, <a href="#BB3_40" class="bgscroll"> BB3_40</a>, <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_10</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_11</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a>, C-PPP_12 (+ #97)</br>
 +
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 +
<br>• Transformation and OneTaq colony PCR of yeast</br>
 +
<div id="BB3_27" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/34/T--BOKU-Vienna--BB327.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_36" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/b/b6/T--BOKU-Vienna--BB336.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_37" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/d/d9/T--BOKU-Vienna--BB337.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_38" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/ad/T--BOKU-Vienna--BB338.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_39" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/d/d2/T--BOKU-Vienna--BB339.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_40" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/e/e0/T--BOKU-Vienna--BB340.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_43" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/f/fe/T--BOKU-Vienna--BB343.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_10" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/9/9f/T--BOKU-Vienna--CPPP10.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
  
 +
<a href="#week12" id="Week 12" class="arrows" onclick="expand_collapse('12');"><pre><font size="+2"><b>Week 12</b></font> (18/09-22/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
 +
<div id="12" class="yolo" style="display:none;">
 +
<p>
 +
<br>• PCR: #102, #103, #105, #106</br>
 +
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 +
<br><br>• Golden Gate Assembly of <a href="#BB2_56" class="bgscroll">BB2_56</a>, <a href="#BB3_09" class="bgscroll"> BB3_09</a>, <a href="#BB3_42" class="bgscroll"> BB3_42</a>, C-PPP_15 (unsuccessful), <a href="#C-PPP_25" class="bgscroll"> C-PPP_26</a>, C-PPP_27 (unsuccessful), <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 +
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 +
<br>• Yeast S288C were made electro-competent.</br>
 +
<br>• Toulouse Collaboration: For further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
  
−
<p></p>
+
<div id="BB2_56" class="modalDialog ">
−
<p></p>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<div id="mydiv">
+
<img src="https://static.igem.org/mediawiki/2017/e/ea/T--BOKU-Vienna--BB256.png" style="width:100%;height:auto;"/>
−
<a name="721" class="anchor"></a>
+
</div></div>
−
  <h4>Friday, July 21st.</h4>
+
<div id="BB3_09" class="modalDialog ">
−
  <ul class="mylist">
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>Minipreparation:</li>
+
<img src="https://static.igem.org/mediawiki/2017/6/6a/T--BOKU-Vienna--BB309.png" style="width:100%;height:auto;"/>
−
      <ul>
+
</div></div>
−
        <li>BB2_11A: Christina</li>
+
<div id="BB3_42" class="modalDialog ">
−
      </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>BB3_5: Yeast in LB Hygromycin</li>
+
<img src="https://static.igem.org/mediawiki/2017/7/76/T--BOKU-Vienna--BB342.png" style="width:100%;height:auto;"/>
−
    <li>Colony PCR</li>
+
</div></div>
−
      <ul><li>Rebecca, Felicia, Melanie, Christina, Mathias</li></ul>
+
<div id="C-PPP_25" class="modalDialog ">
−
    <li>Golden Gate PCR - CRISPR</li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
      <ul><li>Christina, Magdalena, Mathias</li></ul>
+
<img src="https://static.igem.org/mediawiki/2017/0/02/T--BOKU-Vienna--CPPP25.png" style="width:100%;height:auto;"/>
−
  </ul>
+
</div></div>
−
</div>
+
<div id="C-PPP_28" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/ac/T--BOKU-Vienna--CPPP28.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_31" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/2c/T--BOKU-Vienna--CPPP31.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_34" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/35/T--BOKU-Vienna--CPPP34.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>    
  
 +
<a href="#week13" id="Week 13" class="arrows" onclick="expand_collapse('13');"><pre><font size="+2"><b>Week 13</b></font> (25/09-29/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
 +
<div id="13" class="yolo" style="display:none;">
 +
<p>
 +
<br>• PCR: #5, #110, #126, #127, #131</br>
 +
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 +
<br><br>• Golden Gate Assembly of <a href="#BB1_32" class="bgscroll"> BB1_32</a>, <a href="#BB2_51" class="bgscroll"> BB2_51</a>, <a href="#BB2_52" class="bgscroll"> BB2_52</a>, <a href="#BB2_53" class="bgscroll"> BB2_53</a>, <a href="#BB2_54" class="bgscroll"> BB2_54</a>, <a href="#BB2_55" class="bgscroll"> BB2_55</a>, <a href="#BB2_57" class="bgscroll"> BB2_57</a>, BB3_41 (unsuccessful), <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#BB3_45" class="bgscroll"> BB3_45</a>, <a href="#BB3_46" class="bgscroll"> BB3_46</a>, <a href="#BB3_47" class="bgscroll"> BB3_47</a>, <a href="#BB3_48" class="bgscroll"> BB3_48</a>, <a href="#BB3_49" class="bgscroll"> BB3_49</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_14</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_15</a>, <a href="#C-PPP_16" class="bgscroll"> C-PPP_16</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_19</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_20</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_21</a>, <a href="#C-PPP_22" class="bgscroll"> C-PPP_22</a>, <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 +
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 +
<br>• Heidelberg Collaboration: For further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
  
−
<p></p>
+
<div id="BB1_32" class="modalDialog ">
−
<p></p>
+
<div><a href="#close" title="Close" class="close">X</a>
−
<div id="mydiv">
+
<img src="https://static.igem.org/mediawiki/2017/9/9a/T--BOKU-Vienna--BB132.png" style="width:100%;"/>
−
<a name="724" class="anchor"></a>
+
</div></div>
−
  <h4>Monday, July 24th.</h4>
+
<div id="BB2_51" class="modalDialog ">
−
  <ul class="mylist">
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>Colony PCR: CRISPR (4x), BB3_5 (6x)</li>
+
<img src="https://static.igem.org/mediawiki/2017/a/a8/T--BOKU-Vienna--BB251.png" style="width:100%;height:auto;"/>
−
      <ul>
+
</div></div>
−
        <li>Lisa, Valentin</li>
+
<div id="BB2_52" class="modalDialog ">
−
      </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>PCR: yeast sgRNA URA3, yeast sgRNA LEV2</li>
+
<img src="https://static.igem.org/mediawiki/2017/e/e2/T--BOKU-Vienna--BB252.png" style="width:100%;height:auto;"/>
−
      <ul>
+
</div></div>
−
      <li>Mathias, Christina, Melanie</li>
+
<div id="BB2_53" class="modalDialog ">
−
      </ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
    <li>Golden Gate</li>
+
<img src="https://static.igem.org/mediawiki/2017/d/d3/T--BOKU-Vienna--BB253.png" style="width:100%;height:auto;"/>
−
      <ul><li>BB3_2, BB3_1, BB3_3, BB3_4, BB3_6, BB2_18, BB3_8</li></ul>
+
</div></div>
−
      <ul><li>Christina, Mathias</li></ul>
+
<div id="BB2_54" class="modalDialog ">
−
    <li>Golden Gate</li>
+
<div><a href="#close" title="Close" class="close">X</a>
−
        <ul><li>BB1_16, BB1_17</li></ul>
+
<img src="https://static.igem.org/mediawiki/2017/3/35/T--BOKU-Vienna--BB254.png" style="width:100%;height:auto;"/>
−
        <ul><li>Mina, Felicia</li></ul>
+
</div></div>
−
  </ul>
+
<div id="BB2_55" class="modalDialog ">
−
</div>
+
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/1/1f/T--BOKU-Vienna--BB255.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB2_57" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/3e/T--BOKU-Vienna--BB257.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_43" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/f/fe/T--BOKU-Vienna--BB343.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_45" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/9/99/T--BOKU-Vienna--BB345.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_46" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/0/0f/T--BOKU-Vienna--BB346.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_47" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/7/74/T--BOKU-Vienna--BB347.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_48" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/8/89/T--BOKU-Vienna--BB348.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="BB3_49" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/0/09/T--BOKU-Vienna--BB349.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_13" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/1/1c/T--BOKU-Vienna--CPPP13.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_19" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/1/1d/T--BOKU-Vienna--CPPP19.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_22" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/1/1b/T--BOKU-Vienna--CPPP22.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_28" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/a/ac/T--BOKU-Vienna--CPPP28.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_31" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/2/2c/T--BOKU-Vienna--CPPP31.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
<div id="C-PPP_34" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/3/35/T--BOKU-Vienna--CPPP34.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
  
−
<p></p>
+
<a href="#week14" id="Week 14" class="arrows" onclick="expand_collapse('14');"><pre><font size="+2"><b>Week 14</b></font> (02/10-06/10)</pre></a><span style="font-size:150%;"><b> </b></span>
−
<div id="mydiv">
+
<div id="14" class="yolo" style="display:none;">
−
<a name="725" class="anchor"></a>
+
<p>
−
  <h4>Tuesday, July 25th.</h4>
+
<br>• Golden Gate Assembly of <a href="#C-PPP_37" class="bgscroll"> C-PPP_37</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
−
  <ul class="mylist">
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.
−
    <li>Minipreparation of BB3_5B and BB3_5F:</li>
+
<div id="C-PPP_37" class="modalDialog ">
−
      <ul>
+
<div><a href="#close" title="Close" class="close">X</a>
−
        <li>Rebecca</li>
+
<img src="https://static.igem.org/mediawiki/2017/d/dd/T--BOKU-Vienna--CPPP37.png" style="width:100%;height:auto;"/>
−
      </ul>
+
</div></div>
−
    <li>OneTaq PCR from colonies of BB3_1; BB3_2; BB3_8 and plated onto petri dishes with LB-Hygromycine</li>
+
</p></div>
−
      <ul>
+
−
      <li> Lisa, Magdalena, Felicia, Mathias, Melanie, Christina</li>
+
−
      </ul>
+
−
    <li>Some agar plates (BB3_1 + 2 + 8) had too many colonies, so single colonies were plated onto new agar plates</li>
+
−
      <ul><li>Felicia, Josef, Magda</li></ul>
+
−
    <li>Analytical gel electrophoresis from previous OneTaq PCRs</li>
+
−
        <ul><li>Julian, Felicia, Christina</li></ul>
+
−
    <li>Transformation (BB1_16, BB1_17 → Hefe guide RNA)</li>
+
−
      <ul>
+
−
        <li> Julian, Josef</li>
+
−
      </ul>
+
−
  </ul>
+
−
</div>
+
  
 +
<a href="#week15" id="Week 15" class="arrows" onclick="expand_collapse('15');"><pre><font size="+2"><b>Week 15</b></font> (09/10-13/10)</pre></a><span style="font-size:150%;"><b>  </b></span>
 +
<div id="15" class="yolo" style="display:none;">
 +
<p>
 +
<br>• Lethality Test of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
 +
<br>• RT activity assay </br>
 +
<br>• Lethality Test of C-PPP 24 and 30 </br>
 +
<br>• Transformation of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> into <i>E.coli</i> with pSIM9.</br>
 +
Positive clones were made electro-competent and the lambda-red system was induced. </br>
 +
<br>• Integration: GFPuv into <i>E. coli</i> DH10B using <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> and pSIM9</br>
 +
<div id="C-PPP_37" class="modalDialog ">
 +
<div><a href="#close" title="Close" class="close">X</a>
 +
<img src="https://static.igem.org/mediawiki/2017/d/dd/T--BOKU-Vienna--CPPP37.png" style="width:100%;height:auto;"/>
 +
</div></div>
 +
</p></div>
 +
   
 
<p></p>
 
<p></p>
−
<div id="mydiv">
 
−
<a name="726" class="anchor"></a>
 
−
  <h4>Wednesday, July 26th.</h4>
 
−
  <ul class="mylist">
 
−
    <li>Colony PCR: BB1_16, BB1_17, BB3_1 + 2 + 8 and plasmid preparation</li>
 
−
      <ul>
 
−
        <li>Magdalena, Lisa, Valentin, Josef</li>
 
−
      </ul>
 
−
    <li>Golden Gate with URA3</li>
 
−
      <ul>
 
−
      <li>Josef, Julian, Christina, Rebecca</li>
 
−
      </ul>
 
−
    <li>Miniprep: </li>
 
−
      <ul><li>BB2_18A; BB2_18D; BB2_18F: Melanie, Felicia</li></ul>
 
−
      <ul><li>BB2_18A; BB2_18D; BB2_18F: Valentin, Mathias</li></ul>
 
−
    <li>PCR: #38, #39, #40</li>
 
−
    <li>Gel/PCR DNA Extraction:</li>
 
−
      <ul>
 
−
        <li> Felicia</li>
 
−
      </ul>
 
−
    <li>OD Measurement of preculture from SC288:</li>
 
−
      <ul>
 
−
          <li>Mathias, Melanie</li>
 
−
      </ul>
 
−
  </ul>
 
−
</div>
 
−
 
 
<p></p>
 
<p></p>
−
<div id="mydiv">
 
−
<a name="727" class="anchor"></a>
 
−
  <h4>Thursday, July 27th.</h4>
 
−
  <ul class="mylist">
 
−
    <li>Colony PCR: BB3_1, 5, 2; BB2_17; BB1_18; CRISPR-PPP</li>
 
−
      <ul>
 
−
        <li>Magdalena, Felicia, Lisa, Mina</li>
 
−
      </ul>
 
−
    <li>Miniprep from BB1_16, 17</li>
 
−
      <ul>
 
−
      <li>Magdalena, Lisa</li>
 
−
      </ul>
 
−
    <li>PCR #41-43, 31-36 including Gel electrophoresis and gel DNA extraction</li>
 
−
      <ul><li>Felicia, Rebecca, Christina, Mathias, Julian </li></ul>
 
−
    <li>PCR: #38, #39, #40</li>
 
−
    <li>Golden Gate</li>
 
−
      <ul>
 
−
        <li>BB2_10</li>
 
−
        <li>BB2_19 and BB2_10: Josef, Felicia</li>
 
−
      </ul>
 
−
    <li>Preparing LB-Amp agar plates</li>
 
−
      <ul>
 
−
          <li>Melanie, Rebecca, Josef</li>
 
−
      </ul>
 
−
  </ul>
 
−
</div>
 
  
−
<p></p>
 
−
<div id="mydiv">
 
−
<a name="728" class="anchor"></a>
 
−
  <h4>Friday, July 28th.</h4>
 
−
  <ul class="mylist">
 
−
    <li>Colony PCR</li>
 
−
        <ul><li>BB1_20 - BB1_25, BB2_23, BB2_24, BB3_8 + preculture for miniprep in Kanamycin, Hygromycin & Ampicillin Medium</li></ul>
 
−
        <ul><li>Magdalena, Felicia, Rebecca</li></ul>
 
−
        <ul><li>Preculture for miniprep: BB3_1, BB3_5 (Magdalena, Lisa)<li>
 
−
       
 
−
      </ul>
 
−
    <li>Miniprep: CRISPR-PPP; BB3_2; BB1_18</li>
 
−
      <ul>
 
−
      <li>Lisa, Mina</li>
 
−
      </ul>
 
−
    <li>Sequencing BB3_8</li>
 
−
      <ul><li>Christina</li></ul>
 
−
    <li>Transformation</li>
 
−
        <ul><li>BB2_10,19; BB1_20-25</li></ul>
 
−
    <li>LB-Kanamycin plates + agarose gel for gel electrophoresis</li>
 
−
      <ul>
 
−
        <li>Valentin</li>
 
−
      </ul>
 
−
    <li>Gel electrophoresis</li>
 
−
      <ul>
 
−
          <li>CRISPR, BB1_18, BB3_2, BB2_2 and gel DNA extraction</li>
 
−
          <li>Christina, Mathias</li>
 
−
          <li>BB1_20, 21, 22, 23, 24, BB1_25, BB2_23, BB2_24, BB3_8</li>
 
−
          <li>Lisa, Felicia, Rebecca</li>
 
−
          <li>BB3_1, BB3_5</li>
 
−
          <li>Christina</li>
 
−
      </ul>
 
−
    <li>Repetition of colony PCR: BB2_23 and BB3_8</li>
 
−
  </ul>
 
−
</div>
 
−
 
−
<p></p>
 
−
<div id="mydiv">
 
−
<a name="731" class="anchor"></a>
 
−
  <h4>Monday, July 31st.</h4>
 
−
  <ul class="mylist">
 
−
    <li>Colony PCR</li>
 
−
        <ul><li>BB2_10; BB2_19; BB2_20; BB3_1; BB3_6; BB3_8 and miniprep pre-cultures in Amp- or Kan- Media</li></ul>
 
−
        <ul><li>Lisa, Magda, Mina, Rebecca, Felicia</li></ul>
 
−
 
 
−
    <li>Golden Gate Assembly:</li>
 
−
      <ul>
 
−
      <li>BB2 25-30, CRISPR 2-3 & BB3_2 + Amp, BB1_19</li>
 
−
      </ul>
 
−
      <ul>
 
−
      <li> Josef, Rebecca, Lisa, Christina, Mathias</li>
 
−
      </ul>
 
−
      <li>Transformation of E. Coli BB2_20 +28+30, BB2_27 + 29 + CRISPR 2 & 3</li>
 
−
    <li>Repetition of colony PCR: BB2_19 with new primers (41 fw, 42 rev)</li>
 
−
      <ul><li>Magda, Lisa, Mina, Rebecca</li></ul>
 
−
    <li>Gel electrophoresis</li>
 
−
        <ul><li> Felicia, Josef</li></ul>
 
−
    <li>Transformation of yeast</li>
 
−
      <ul>
 
−
        <li>Lisa, Michael, Rebecca</li>
 
−
      </ul>
 
−
  </ul>
 
−
</div>
 
  
−
</body>
 
  
  

Latest revision as of 20:56, 1 November 2017

Notebook

V

Notebook.


All protocols we used for the methods can be found here: Protocol

For further information regarding our Golden Gate Assembly products, we refer to our part collection site. The list of our primers can be found here. 

Week 1 (03/07-07/07)
 
Week 2 (10/07-14/07)
 
Week 3 (17/07-21/07)
 
Week 4 (24/07-28/07)
 
Week 5 (31/07-04/08)
 
Week 6 (07/08-11/08)
 
Week 7 (14/08-18/08)
 
Week 8 (21/08-25/08)
 
Week 9 (28/08-01/09)
 
Week 10 (04/09-08/09)
 
Week 11 (11/09-15/09)
 
Week 12 (18/09-22/09)
 
Week 13 (25/09-29/09)
 
Week 14 (02/10-06/10)
 
Week 15 (09/10-13/10)