Difference between revisions of "Team:BOKU-Vienna/Notebook"

 
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<a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol" target="_blank">Protocol</a>
 
<a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol" target="_blank">Protocol</a>
 
                 </br>
 
                 </br>
                     <br>For further information regarding our Golden Gate Assembly products, please refer to our <a href="https://2017.igem.org/Team:BOKU-Vienna/Part_Collection" target="_blank">part collection</a> site. The list of our primers can be found <a href="https://static.igem.org/mediawiki/2017/e/ea/T--BOKU-Vienna--Primerliste.pdf" target="_blank">here</a>.
+
                     <br>For further information regarding our Golden Gate Assembly products, we refer to our <a href="https://2017.igem.org/Team:BOKU-Vienna/Part_Collection" target="_blank">part collection</a> site. The list of our primers can be found <a href="https://static.igem.org/mediawiki/2017/e/ea/T--BOKU-Vienna--Primerliste.pdf" target="_blank">here</a>.
 
</p>
 
</p>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
  
<a href="#izhfd" id="scrollnav" class="arrows" onclick="expand_collapse('1');"><pre><font size="+2"><b>Week 1</b></font> (03/07-07/07)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week1" id="scrollnav" class="arrows" onclick="expand_collapse('1');"><pre><font size="+2"><b>Week 1</b></font> (03/07-07/07)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="1" class="yolo" style="display:none;">
 
<div id="1" class="yolo" style="display:none;">
 
   <p>
 
   <p>
 
<br>  • Preparation of growth media </br>
 
<br>  • Preparation of growth media </br>
<br>  • Interlab Challenge 2017: Transformations of the plasmids into <i>E. coli</i> DH5α <br>for more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site. </br>
+
<br>  • Interlab Challenge 2017: Transformations of the plasmids into <i>E. coli</i> DH5α. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site. </br>
<br>  • <i>E. Coli</i> DH10B cells made chemically competent</br>
+
<br>  • <i>E. Coli</i> DH10B cells were made chemically competent.</br>
 
<br>  • Miniprep of GG plasmids:
 
<br>  • Miniprep of GG plasmids:
 
<a href="#BB1_01" class="bgscroll"> BB1_01</a>, <a href="#BB1_02" class="bgscroll"> BB1_02</a>, <a href="#BB1_03" class="bgscroll"> BB1_03</a>, <a href="#BB1_04" class="bgscroll"> BB1_04</a>, <a href="#BB1_05" class="bgscroll"> BB1_05</a>, <a href="#BB1_06" class="bgscroll"> BB1_06</a>, <a href="#BB1_07" class="bgscroll"> BB1_07</a>, <a href="#BB1_08" class="bgscroll"> BB1_08</a></br>
 
<a href="#BB1_01" class="bgscroll"> BB1_01</a>, <a href="#BB1_02" class="bgscroll"> BB1_02</a>, <a href="#BB1_03" class="bgscroll"> BB1_03</a>, <a href="#BB1_04" class="bgscroll"> BB1_04</a>, <a href="#BB1_05" class="bgscroll"> BB1_05</a>, <a href="#BB1_06" class="bgscroll"> BB1_06</a>, <a href="#BB1_07" class="bgscroll"> BB1_07</a>, <a href="#BB1_08" class="bgscroll"> BB1_08</a></br>
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<a href="#adrfou" id="Week 2" class="arrows" onclick="expand_collapse('2');"><pre><font size="+2"><b>Week 2</b></font> (10/07-14/07)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week2" id="Week 2" class="arrows" onclick="expand_collapse('2');"><pre><font size="+2"><b>Week 2</b></font> (10/07-14/07)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="2" class="yolo" style="display:none;">
 
<div id="2" class="yolo" style="display:none;">
 
<p>
 
<p>
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Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of <a href="#BB1_09" class="bgscroll"> BB1_09</a>, <a href="#BB1_10" class="bgscroll"> BB1_10</a>, <a href="#BB1_11" class="bgscroll"> BB1_11</a>, <a href="#BB1_12" class="bgscroll"> BB1_12</a>, <a href="#BB1_13" class="bgscroll"> BB1_13</a>, BB2_01 (unsuccessful), <a href="#BB2_02" class="bgscroll"> BB2_02</a>, <a href="#BB2_03" class="bgscroll"> BB2_03</a>, <a href="#BB2_04" class="bgscroll"> BB2_04</a>, BB2_05 (unsuccessful)</br>
 
<br><br>• Golden Gate Assembly of <a href="#BB1_09" class="bgscroll"> BB1_09</a>, <a href="#BB1_10" class="bgscroll"> BB1_10</a>, <a href="#BB1_11" class="bgscroll"> BB1_11</a>, <a href="#BB1_12" class="bgscroll"> BB1_12</a>, <a href="#BB1_13" class="bgscroll"> BB1_13</a>, BB2_01 (unsuccessful), <a href="#BB2_02" class="bgscroll"> BB2_02</a>, <a href="#BB2_03" class="bgscroll"> BB2_03</a>, <a href="#BB2_04" class="bgscroll"> BB2_04</a>, BB2_05 (unsuccessful)</br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
 
<br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
 
<br>• Transformation of HSM174 + pSIM5 (unsuccessful)</br>
 
<br>• Transformation of HSM174 + pSIM5 (unsuccessful)</br>
<br>• Yeast S288C gDNA extracted </br>
+
<br>• Yeast S288C gDNA extraction </br>
 +
 
 +
<br>• Interlab Challenge 2017: Performance of the measurements. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site.</br>
 +
 
  
 
<div id="BB1_09" class="modalDialog ">
 
<div id="BB1_09" class="modalDialog ">
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</p></div>
 
</p></div>
  
<a href="#nxbfgr" id="Week 3" class="arrows" onclick="expand_collapse('3');"><pre><font size="+2"><b>Week 3</b></font> (17/07-21/07)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week3" id="Week 3" class="arrows" onclick="expand_collapse('3');"><pre><font size="+2"><b>Week 3</b></font> (17/07-21/07)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="3" class="yolo" style="display:none;">
 
<div id="3" class="yolo" style="display:none;">
 
<p>
 
<p>
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Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>, BB2_01 (unsuccessful, even with new BBa_23101 promoter), BB2_05 (unsuccessful), <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a>, <a href="#BB2_14" class="bgscroll"> BB2_14</a>, <a href="#BB2_15" class="bgscroll"> BB2_15</a>, <a href="#BB2_16" class="bgscroll"> BB2_16</a>, C-PPP_01 (unsuccessful)</br>
 
<br><br>• Golden Gate Assembly of <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>, BB2_01 (unsuccessful, even with new BBa_23101 promoter), BB2_05 (unsuccessful), <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a>, <a href="#BB2_14" class="bgscroll"> BB2_14</a>, <a href="#BB2_15" class="bgscroll"> BB2_15</a>, <a href="#BB2_16" class="bgscroll"> BB2_16</a>, C-PPP_01 (unsuccessful)</br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Sent for Sequencing: <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>,  <a href="#BB2_01" class="bgscroll"> BB2_01</a>, <a href="#BB2_05" class="bgscroll"> BB2_05</a>, <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a></br>
 
<br>• Sent for Sequencing: <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>,  <a href="#BB2_01" class="bgscroll"> BB2_01</a>, <a href="#BB2_05" class="bgscroll"> BB2_05</a>, <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a></br>
<br>• Interlab Challenge 2017: Performance of the measurements. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site.</br>
+
 
  
 
<div id="BB1_14" class="modalDialog ">
 
<div id="BB1_14" class="modalDialog ">
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</p></div>
 
</p></div>
  
<a href="#iqiuoy" id="Week 4" class="arrows" onclick="expand_collapse('4');"><pre><font size="+2"><b>Week 4</b></font> (24/07-28/07)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week4" id="Week 4" class="arrows" onclick="expand_collapse('4');"><pre><font size="+2"><b>Week 4</b></font> (24/07-28/07)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="4" class="yolo" style="display:none;">
 
<div id="4" class="yolo" style="display:none;">
 
<p>
 
<p>
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GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Yeast S266C made electro-competent</br>
+
<br>• Yeast S266C were made electro-competent.</br>
 
<br>• Sent for Sequencing: <a href="#BB1_16" class="bgscroll"> BB1_16</a>, <a href="#BB1_17" class="bgscroll"> BB1_17</a>, <a href="#BB1_18" class="bgscroll"> BB1_18</a>, <a href="#BB3_02" class="bgscroll"> BB3_02</a>, <a href="#BB3_05" class="bgscroll"> BB3_05</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_01</a></br>
 
<br>• Sent for Sequencing: <a href="#BB1_16" class="bgscroll"> BB1_16</a>, <a href="#BB1_17" class="bgscroll"> BB1_17</a>, <a href="#BB1_18" class="bgscroll"> BB1_18</a>, <a href="#BB3_02" class="bgscroll"> BB3_02</a>, <a href="#BB3_05" class="bgscroll"> BB3_05</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_01</a></br>
  
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<a href="#kskjdh" id="Week 5" class="arrows" onclick="expand_collapse('5');"><pre><font size="+2"><b>Week 5</b></font> (31/07-04/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week5" id="Week 5" class="arrows" onclick="expand_collapse('5');"><pre><font size="+2"><b>Week 5</b></font> (31/07-04/08)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="5" class="yolo" style="display:none;">
 
<div id="5" class="yolo" style="display:none;">
 
<p>
 
<p>
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Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of <a href="#BB1_19" class="bgscroll"> BB1_19</a>, <a href="#BB2_19" class="bgscroll"> BB2_19</a>, <a href="#BB2_25" class="bgscroll"> BB2_25</a>, <a href="#BB2_26" class="bgscroll"> BB2_26</a>, <a href="#BB2_27" class="bgscroll"> BB2_27</a>, <a href="#BB2_28" class="bgscroll"> BB2_28</a>, <a href="#BB2_29" class="bgscroll"> BB2_29</a>, <a href="#BB2_30" class="bgscroll"> BB2_30</a>, <a href="#BB2_31" class="bgscroll"> BB2_31</a>, <a href="#BB2_32" class="bgscroll"> BB2_32</a>, <a href="#BB2_33" class="bgscroll"> BB2_33</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, BB3_01 (unsuccessful), <a href="#BB3_02" class="bgscroll"> BB3_02</a>, BB3_03 (unsuccessful), <a href="#BB3_06" class="bgscroll"> BB3_06</a>, <a href="#BB3_10" class="bgscroll"> BB3_10</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#BB3_15" class="bgscroll"> BB3_15</a>, <a href="#BB3_24" class="bgscroll"> BB3_24</a></br>
 
<br><br>• Golden Gate Assembly of <a href="#BB1_19" class="bgscroll"> BB1_19</a>, <a href="#BB2_19" class="bgscroll"> BB2_19</a>, <a href="#BB2_25" class="bgscroll"> BB2_25</a>, <a href="#BB2_26" class="bgscroll"> BB2_26</a>, <a href="#BB2_27" class="bgscroll"> BB2_27</a>, <a href="#BB2_28" class="bgscroll"> BB2_28</a>, <a href="#BB2_29" class="bgscroll"> BB2_29</a>, <a href="#BB2_30" class="bgscroll"> BB2_30</a>, <a href="#BB2_31" class="bgscroll"> BB2_31</a>, <a href="#BB2_32" class="bgscroll"> BB2_32</a>, <a href="#BB2_33" class="bgscroll"> BB2_33</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, BB3_01 (unsuccessful), <a href="#BB3_02" class="bgscroll"> BB3_02</a>, BB3_03 (unsuccessful), <a href="#BB3_06" class="bgscroll"> BB3_06</a>, <a href="#BB3_10" class="bgscroll"> BB3_10</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#BB3_15" class="bgscroll"> BB3_15</a>, <a href="#BB3_24" class="bgscroll"> BB3_24</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Preculture of DH10B <i>E. coli</i> strain made chemically competent</br>
+
<br>• DH10B <i>E. coli</i> strain was made chemically competent.</br>
<br>• Yeast cells transformed with the Cas9 plasmid</br>
+
<br>• Yeast cells were transformed with the Cas9 plasmid.</br>
 
<br>• Sent for Sequencing: <a href="#BB1_19" class="bgscroll"> BB1_19</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_02</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a></br>
 
<br>• Sent for Sequencing: <a href="#BB1_19" class="bgscroll"> BB1_19</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_02</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a></br>
  
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</p></div>
 
</p></div>
  
<a href="#jgcfgr" id="Week 6" class="arrows" onclick="expand_collapse('6');"><pre><font size="+2"><b>Week 6</b></font> (07/08-11/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week6" id="Week 6" class="arrows" onclick="expand_collapse('6');"><pre><font size="+2"><b>Week 6</b></font> (07/08-11/08)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="6" class="yolo" style="display:none;">
 
<div id="6" class="yolo" style="display:none;">
 
<p>
 
<p>
 
<br>• Golden Gate Assembly of <a href="#BB2_36" class="bgscroll"> BB2_36</a>, <a href="#BB3_07" class="bgscroll"> BB3_07</a>, <a href="#BB3_10" class="bgscroll"> BB3_10</a>, <a href="#BB3_11" class="bgscroll"> BB3_11</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_18" class="bgscroll"> BB3_18</a>, <a href="#BB3_19" class="bgscroll"> BB3_19</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_21" class="bgscroll"> BB3_21</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, <a href="#BB3_23" class="bgscroll"> BB3_23</a>, <a href="#BB3_24" class="bgscroll"> BB3_24</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a></br>
 
<br>• Golden Gate Assembly of <a href="#BB2_36" class="bgscroll"> BB2_36</a>, <a href="#BB3_07" class="bgscroll"> BB3_07</a>, <a href="#BB3_10" class="bgscroll"> BB3_10</a>, <a href="#BB3_11" class="bgscroll"> BB3_11</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_18" class="bgscroll"> BB3_18</a>, <a href="#BB3_19" class="bgscroll"> BB3_19</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_21" class="bgscroll"> BB3_21</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, <a href="#BB3_23" class="bgscroll"> BB3_23</a>, <a href="#BB3_24" class="bgscroll"> BB3_24</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected  by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected  by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• A temperature-sensitivity test was performed for cells with pSIM5, a temperature sensitive plasmid. An overnight culture was incubated at 37°C and a control was incubated at 28°C. The overnight culture at 37°C was tested as positive and lost the plasmid, while the control maintained its plasmid.</br>
 
<br>• A temperature-sensitivity test was performed for cells with pSIM5, a temperature sensitive plasmid. An overnight culture was incubated at 37°C and a control was incubated at 28°C. The overnight culture at 37°C was tested as positive and lost the plasmid, while the control maintained its plasmid.</br>
<br>• Two clones of yeast cells carrying the Cas9 plasmid made competent for the next transformation</br>
+
<br>• Two clones of yeast cells carrying the Cas9 plasmid were made competent for the next transformation.</br>
 
<br>• Sent for Sequencing: <a href="#BB2_19" class="bgscroll"> BB2_19</a>, <a href="#BB2_21" class="bgscroll"> BB2_21</a>, <a href="#BB2_31" class="bgscroll"> BB2_31</a>, <a href="#BB2_32" class="bgscroll"> BB2_32</a>, <a href="#BB2_33" class="bgscroll"> BB2_33</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, <a href="#BB3_01" class="bgscroll"> BB3_01</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_02</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a></br>
 
<br>• Sent for Sequencing: <a href="#BB2_19" class="bgscroll"> BB2_19</a>, <a href="#BB2_21" class="bgscroll"> BB2_21</a>, <a href="#BB2_31" class="bgscroll"> BB2_31</a>, <a href="#BB2_32" class="bgscroll"> BB2_32</a>, <a href="#BB2_33" class="bgscroll"> BB2_33</a>, <a href="#BB2_34" class="bgscroll"> BB2_34</a>, <a href="#BB3_01" class="bgscroll"> BB3_01</a>, <a href="#BB3_13" class="bgscroll"> BB3_13</a>, <a href="#BB3_14" class="bgscroll"> BB3_14</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_02</a>, <a href="#C-PPP_01" class="bgscroll"> C-PPP_03</a></br>
  
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</p></div>
 
</p></div>
 
                  
 
                  
<a href="#iffuwy" id="Week 7" class="arrows" onclick="expand_collapse('7');"><pre><font size="+2"><b>Week 7</b></font> (14/08-18/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week7" id="Week 7" class="arrows" onclick="expand_collapse('7');"><pre><font size="+2"><b>Week 7</b></font> (14/08-18/08)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="7" class="yolo" style="display:none;">
 
<div id="7" class="yolo" style="display:none;">
 
<p>
 
<p>
 
<br>• Golden Gate Assembly of <a href="#BB1_26" class="bgscroll"> BB1_26</a>, <a href="#BB2_23" class="bgscroll"> BB2_23</a>, BB3_02 (unsuccessful), <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_16" class="bgscroll"> BB3_16</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, BB3_28 (unsuccessful), <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a></br>
 
<br>• Golden Gate Assembly of <a href="#BB1_26" class="bgscroll"> BB1_26</a>, <a href="#BB2_23" class="bgscroll"> BB2_23</a>, BB3_02 (unsuccessful), <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_16" class="bgscroll"> BB3_16</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, BB3_28 (unsuccessful), <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Overnight cultures of <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a> tested for temperature sensitivity</br>
+
<br>• Overnight cultures of <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a> were tested for temperature sensitivity.</br>
 
<br>• Sent for Sequencing: <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a></br>
 
<br>• Sent for Sequencing: <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a></br>
  
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</p></div>
 
</p></div>
  
<a href="#kskjdh" id="Week 8" class="arrows" onclick="expand_collapse('8');"><pre><font size="+2"><b>Week 8</b></font> (21/08-25/08)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week8" id="Week 8" class="arrows" onclick="expand_collapse('8');"><pre><font size="+2"><b>Week 8</b></font> (21/08-25/08)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="8" class="yolo" style="display:none;">
 
<div id="8" class="yolo" style="display:none;">
 
<p>
 
<p>
<br>• PCR: DH5-α pSIM5, #71, #72</br>
+
<br>• PCR: #71, #72</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to protocol.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to protocol.
 
<br><br>• Golden Gate Assembly of <a href="#BB1_27" class="bgscroll"> BB1_27</a>, BB3_01 (unsuccessful), <a href="#BB3_03" class="bgscroll"> BB3_03</a>, <a href="#BB3_30" class="bgscroll"> BB3_30</a>, <a href="#BB3_31" class="bgscroll"> BB3_31</a>, <a href="#BB3_32" class="bgscroll"> BB3_32</a>, <a href="#BB3_33" class="bgscroll"> BB3_33</a></br>
 
<br><br>• Golden Gate Assembly of <a href="#BB1_27" class="bgscroll"> BB1_27</a>, BB3_01 (unsuccessful), <a href="#BB3_03" class="bgscroll"> BB3_03</a>, <a href="#BB3_30" class="bgscroll"> BB3_30</a>, <a href="#BB3_31" class="bgscroll"> BB3_31</a>, <a href="#BB3_32" class="bgscroll"> BB3_32</a>, <a href="#BB3_33" class="bgscroll"> BB3_33</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Transformation of <a href="#BB3_32" class="bgscroll"> BB3_32</a> and <a href="#BB3_31" class="bgscroll"> BB3_31</a> into yeast cells</br>
 
<br>• Transformation of <a href="#BB3_32" class="bgscroll"> BB3_32</a> and <a href="#BB3_31" class="bgscroll"> BB3_31</a> into yeast cells</br>
<br>• <a href="#BB3_31" class="bgscroll"> BB3_31</a> and <a href="#BB3_32" class="bgscroll"> BB3_32</a> cultivated in YP+G418, a yeast peptone media</br> for testing of the GFP expression in a plate reader.  
+
<br>• <a href="#BB3_31" class="bgscroll"> BB3_31</a> and <a href="#BB3_32" class="bgscroll"> BB3_32</a> cultivated in YP+G418: for testing the GFP expression, measurement in a plate reader.  
  
  
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</p></div>
 
</p></div>
  
<a href="#nxbfgr" id="Week 9" class="arrows" onclick="expand_collapse('9');"><pre><font size="+2"><b>Week 9</b></font> (28/08-01/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week9" id="Week 9" class="arrows" onclick="expand_collapse('9');"><pre><font size="+2"><b>Week 9</b></font> (28/08-01/09)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="9" class="yolo" style="display:none;">
 
<div id="9" class="yolo" style="display:none;">
 
<p>
 
<p>
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</br>
 
</br>
  
<br>•Golden Gate Assembly of BB3_01 (unsuccessful), <a href="#C-PPP_07" class="bgscroll">C-PPP_07</a>, <a href="#C-PPP_07" class="bgscroll"> C-PPP_08</a>,  <a href="#C-PPP_07" class="bgscroll"> C-PPP_09</a>, <a href="#C-PPP_10" class="bgscroll">C-PPP_10</a>, <a href="#BB3_33" class="bgscroll">BB3_33</a></br>
+
<br>• Golden Gate Assembly of BB3_01 (unsuccessful), <a href="#C-PPP_07" class="bgscroll">C-PPP_07</a>, <a href="#C-PPP_07" class="bgscroll"> C-PPP_08</a>,  <a href="#C-PPP_07" class="bgscroll"> C-PPP_09</a>, <a href="#C-PPP_10" class="bgscroll">C-PPP_10</a>, <a href="#BB3_33" class="bgscroll">BB3_33</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
  
<br>•Minipreparation for plasmid DNA extraction: <a href="#BB3_12" class="bgscroll">BB3_12</a>, <a href="#BB3_30" class="bgscroll">BB3_30</a>, <a href="#BB3_15" class="bgscroll">BB3_15</a></br>
+
<br>• Minipreparation for plasmid DNA extraction: <a href="#BB3_12" class="bgscroll">BB3_12</a>, <a href="#BB3_30" class="bgscroll">BB3_30</a>, <a href="#BB3_15" class="bgscroll">BB3_15</a></br>
<br>•Transformation: pSIM9</br>
+
<br>• Transformation of pSIM9</br>
  
 
<div id="BB3_35" class="modalDialog ">
 
<div id="BB3_35" class="modalDialog ">
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<a href="#iqiuwy" id="Week 10" class="arrows" onclick="expand_collapse('10');"><pre><font size="+2"><b>Week 10</b></font> (04/09-08/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week10" id="Week 10" class="arrows" onclick="expand_collapse('10');"><pre><font size="+2"><b>Week 10</b></font> (04/09-08/09)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="10" class="yolo" style="display:none;">
 
<div id="10" class="yolo" style="display:none;">
 
<p>
 
<p>
 
<br>• PCR: #88, #89, #90</br>
 
<br>• PCR: #88, #89, #90</br>
  
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of  <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_34" class="bgscroll"> BB3_34</a>, <a href="#BB3_35 Speziale" class="bgscroll"> BB3_35 Speziale</a>, <a href="#BB3_35" class="bgscroll"> BB3_35</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
 
<br><br>• Golden Gate Assembly of  <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_34" class="bgscroll"> BB3_34</a>, <a href="#BB3_35 Speziale" class="bgscroll"> BB3_35 Speziale</a>, <a href="#BB3_35" class="bgscroll"> BB3_35</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Lethality-Test of <a href="#C-PPP_10" class="bgscroll"> C-PPP_11</a> and <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
 
<br>• Lethality-Test of <a href="#C-PPP_10" class="bgscroll"> C-PPP_11</a> and <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
 
<div id="BB3_34" class="modalDialog ">
 
<div id="BB3_34" class="modalDialog ">
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</p></div>
 
</p></div>
  
<a href="#kskjdh" id="Week 11" class="arrows" onclick="expand_collapse('11');"><pre><font size="+2"><b>Week 11</b></font> (11/09-15/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week11" id="Week 11" class="arrows" onclick="expand_collapse('11');"><pre><font size="+2"><b>Week 11</b></font> (11/09-15/09)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="11" class="yolo" style="display:none;">
 
<div id="11" class="yolo" style="display:none;">
 
<p>
 
<p>
<br>• Golden Gate Assembly of <a href="#BB3_27" class="bgscroll"> BB3_27</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#BB3_39" class="bgscroll"> BB3_39</a>, <a href="#BB3_40" class="bgscroll"> BB3_40</a>, <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_10</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_11</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a>, C-PPP_12 (+ #97)</br>
+
<br>• Golden Gate Assembly of <a href="#BB3_27" class="bgscroll"> BB3_27</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#BB3_39" class="bgscroll"> BB3_39</a>, <a href="#BB3_40" class="bgscroll"> BB3_40</a>, <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_10</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_11</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a>, C-PPP_12 (+ #97)</br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Transformation and OneTaq colony PCR of yeast</br>
 
<br>• Transformation and OneTaq colony PCR of yeast</br>
 
<div id="BB3_27" class="modalDialog ">
 
<div id="BB3_27" class="modalDialog ">
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</p></div>
 
</p></div>
  
<a href="#nxbfgr" id="Week 12" class="arrows" onclick="expand_collapse('12');"><pre><font size="+2"><b>Week 12</b></font> (18/09-22/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week12" id="Week 12" class="arrows" onclick="expand_collapse('12');"><pre><font size="+2"><b>Week 12</b></font> (18/09-22/09)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="12" class="yolo" style="display:none;">
 
<div id="12" class="yolo" style="display:none;">
 
<p>
 
<p>
 
<br>• PCR: #102, #103, #105, #106</br>
 
<br>• PCR: #102, #103, #105, #106</br>
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of <a href="#BB2_56" class="bgscroll">BB2_56</a>, <a href="#BB3_09" class="bgscroll"> BB3_09</a>, <a href="#BB3_42" class="bgscroll"> BB3_42</a>, C-PPP_15 (unsuccessful), <a href="#C-PPP_25" class="bgscroll"> C-PPP_26</a>, C-PPP_27 (unsuccessful), <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 
<br><br>• Golden Gate Assembly of <a href="#BB2_56" class="bgscroll">BB2_56</a>, <a href="#BB3_09" class="bgscroll"> BB3_09</a>, <a href="#BB3_42" class="bgscroll"> BB3_42</a>, C-PPP_15 (unsuccessful), <a href="#C-PPP_25" class="bgscroll"> C-PPP_26</a>, C-PPP_27 (unsuccessful), <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Yeast S288C made electro-competent</br>
+
<br>• Yeast S288C were made electro-competent.</br>
<br>• Toulouse Collaboration: for further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
+
<br>• Toulouse Collaboration: For further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
  
 
<div id="BB2_56" class="modalDialog ">
 
<div id="BB2_56" class="modalDialog ">
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</p></div>     
 
</p></div>     
  
<a href="#nxbfgr" id="Week 13" class="arrows" onclick="expand_collapse('13');"><pre><font size="+2"><b>Week 13</b></font> (25/09-29/09)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week13" id="Week 13" class="arrows" onclick="expand_collapse('13');"><pre><font size="+2"><b>Week 13</b></font> (25/09-29/09)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="13" class="yolo" style="display:none;">
 
<div id="13" class="yolo" style="display:none;">
 
<p>
 
<p>
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Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of <a href="#BB1_32" class="bgscroll"> BB1_32</a>, <a href="#BB2_51" class="bgscroll"> BB2_51</a>, <a href="#BB2_52" class="bgscroll"> BB2_52</a>, <a href="#BB2_53" class="bgscroll"> BB2_53</a>, <a href="#BB2_54" class="bgscroll"> BB2_54</a>, <a href="#BB2_55" class="bgscroll"> BB2_55</a>, <a href="#BB2_57" class="bgscroll"> BB2_57</a>, BB3_41 (unsuccessful), <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#BB3_45" class="bgscroll"> BB3_45</a>, <a href="#BB3_46" class="bgscroll"> BB3_46</a>, <a href="#BB3_47" class="bgscroll"> BB3_47</a>, <a href="#BB3_48" class="bgscroll"> BB3_48</a>, <a href="#BB3_49" class="bgscroll"> BB3_49</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_14</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_15</a>, <a href="#C-PPP_16" class="bgscroll"> C-PPP_16</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_19</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_20</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_21</a>, <a href="#C-PPP_22" class="bgscroll"> C-PPP_22</a>, <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 
<br><br>• Golden Gate Assembly of <a href="#BB1_32" class="bgscroll"> BB1_32</a>, <a href="#BB2_51" class="bgscroll"> BB2_51</a>, <a href="#BB2_52" class="bgscroll"> BB2_52</a>, <a href="#BB2_53" class="bgscroll"> BB2_53</a>, <a href="#BB2_54" class="bgscroll"> BB2_54</a>, <a href="#BB2_55" class="bgscroll"> BB2_55</a>, <a href="#BB2_57" class="bgscroll"> BB2_57</a>, BB3_41 (unsuccessful), <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#BB3_45" class="bgscroll"> BB3_45</a>, <a href="#BB3_46" class="bgscroll"> BB3_46</a>, <a href="#BB3_47" class="bgscroll"> BB3_47</a>, <a href="#BB3_48" class="bgscroll"> BB3_48</a>, <a href="#BB3_49" class="bgscroll"> BB3_49</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_14</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_15</a>, <a href="#C-PPP_16" class="bgscroll"> C-PPP_16</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_19</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_20</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_21</a>, <a href="#C-PPP_22" class="bgscroll"> C-PPP_22</a>, <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Heidelberg Collaboration: for further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
+
<br>• Heidelberg Collaboration: For further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
  
 
<div id="BB1_32" class="modalDialog ">
 
<div id="BB1_32" class="modalDialog ">
Line 1,029: Line 1,032:
 
</p></div>
 
</p></div>
  
<a href="#nxbfgr" id="Week 14" class="arrows" onclick="expand_collapse('14');"><pre><font size="+2"><b>Week 14</b></font> (02/10-06/10)</pre></a><span style="font-size:150%;"><b>  </b></span>
+
<a href="#week14" id="Week 14" class="arrows" onclick="expand_collapse('14');"><pre><font size="+2"><b>Week 14</b></font> (02/10-06/10)</pre></a><span style="font-size:150%;"><b> </b></span>
 
<div id="14" class="yolo" style="display:none;">
 
<div id="14" class="yolo" style="display:none;">
 
<p>
 
<p>
 
<br>• Golden Gate Assembly of <a href="#C-PPP_37" class="bgscroll"> C-PPP_37</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
 
<br>• Golden Gate Assembly of <a href="#C-PPP_37" class="bgscroll"> C-PPP_37</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.
+
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.
 
<div id="C-PPP_37" class="modalDialog ">
 
<div id="C-PPP_37" class="modalDialog ">
 
<div><a href="#close" title="Close" class="close">X</a>
 
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<a href="#nxbfgr" id="Week 15" class="arrows" onclick="expand_collapse('15');"><pre><font size="+2"><b>Week 15</b></font> (09/10-13/10)</pre></a><span style="font-size:150%;"><b>  </b></span>
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<a href="#week15" id="Week 15" class="arrows" onclick="expand_collapse('15');"><pre><font size="+2"><b>Week 15</b></font> (09/10-13/10)</pre></a><span style="font-size:150%;"><b> </b></span>
 
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<br>• Lethality Test of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
 
<br>• Lethality Test of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
<br>• Transformation of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> into <i>E.coli</i> with pSIM9</br>
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<br>• RT activity assay </br>
positive clones made electro-competent and induction of the lambda-red system </br>
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<br>• Lethality Test of C-PPP 24 and 30 </br>
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<br>• Transformation of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> into <i>E.coli</i> with pSIM9.</br>
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Positive clones were made electro-competent and the lambda-red system was induced. </br>
 
<br>• Integration: GFPuv into <i>E. coli</i> DH10B using <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> and pSIM9</br>
 
<br>• Integration: GFPuv into <i>E. coli</i> DH10B using <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> and pSIM9</br>
 
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Latest revision as of 20:56, 1 November 2017

Notebook

V

Notebook.


All protocols we used for the methods can be found here: Protocol

For further information regarding our Golden Gate Assembly products, we refer to our part collection site. The list of our primers can be found here.

Week 1 (03/07-07/07)
Week 2 (10/07-14/07)
Week 3 (17/07-21/07)
Week 4 (24/07-28/07)
Week 5 (31/07-04/08)
Week 6 (07/08-11/08)
Week 7 (14/08-18/08)
Week 8 (21/08-25/08)
Week 9 (28/08-01/09)
Week 10 (04/09-08/09)
Week 11 (11/09-15/09)
Week 12 (18/09-22/09)
Week 13 (25/09-29/09)
Week 14 (02/10-06/10)
Week 15 (09/10-13/10)