Difference between revisions of "Team:BOKU-Vienna/Notebook"

 
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<a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol" target="_blank">Protocol</a>
 
<a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol" target="_blank">Protocol</a>
 
                 </br>
 
                 </br>
                     <br>For further information regarding our Golden Gate Assembly products, please refer to our <a href="https://2017.igem.org/Team:BOKU-Vienna/Part_Collection" target="_blank">part collection</a> site. The list of our primers can be found <a href="https://static.igem.org/mediawiki/2017/e/ea/T--BOKU-Vienna--Primerliste.pdf" target="_blank">here</a>.
+
                     <br>For further information regarding our Golden Gate Assembly products, we refer to our <a href="https://2017.igem.org/Team:BOKU-Vienna/Part_Collection" target="_blank">part collection</a> site. The list of our primers can be found <a href="https://static.igem.org/mediawiki/2017/e/ea/T--BOKU-Vienna--Primerliste.pdf" target="_blank">here</a>.
 
</p>
 
</p>
 
             </div>
 
             </div>
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<div id="1" class="yolo" style="display:none;">
 
<div id="1" class="yolo" style="display:none;">
 
   <p>
 
   <p>
<br>  • Preparation of growth media. </br>
+
<br>  • Preparation of growth media </br>
<br>  • Interlab Challenge 2017: Transformations of the plasmids into <i>E. coli</i> DH5α <br>for more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site. </br>
+
<br>  • Interlab Challenge 2017: Transformations of the plasmids into <i>E. coli</i> DH5α. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site. </br>
 
<br>  • <i>E. Coli</i> DH10B cells were made chemically competent.</br>
 
<br>  • <i>E. Coli</i> DH10B cells were made chemically competent.</br>
 
<br>  • Miniprep of GG plasmids:
 
<br>  • Miniprep of GG plasmids:
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<br>• Transformation of HSM174 + pSIM5 (unsuccessful)</br>
 
<br>• Transformation of HSM174 + pSIM5 (unsuccessful)</br>
 
<br>• Yeast S288C gDNA extraction </br>
 
<br>• Yeast S288C gDNA extraction </br>
 +
 +
<br>• Interlab Challenge 2017: Performance of the measurements. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site.</br>
 +
  
 
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<div id="BB1_09" class="modalDialog ">
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GG-products were transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products were transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Sent for Sequencing: <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>,  <a href="#BB2_01" class="bgscroll"> BB2_01</a>, <a href="#BB2_05" class="bgscroll"> BB2_05</a>, <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a></br>
 
<br>• Sent for Sequencing: <a href="#BB1_14" class="bgscroll"> BB1_14</a>, <a href="#BB1_15" class="bgscroll"> BB1_15</a>,  <a href="#BB2_01" class="bgscroll"> BB2_01</a>, <a href="#BB2_05" class="bgscroll"> BB2_05</a>, <a href="#BB2_06" class="bgscroll"> BB2_06</a>, <a href="#BB2_07" class="bgscroll"> BB2_07</a>, <a href="#BB2_08" class="bgscroll"> BB2_08</a>, <a href="#BB2_09" class="bgscroll"> BB2_09</a>, <a href="#BB2_10" class="bgscroll"> BB2_10</a>, <a href="#BB2_12" class="bgscroll"> BB2_12</a></br>
<br>• Interlab Challenge 2017: Performance of the measurements. For more information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">Interlab</a> site.</br>
+
 
  
 
<div id="BB1_14" class="modalDialog ">
 
<div id="BB1_14" class="modalDialog ">
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<br>• Golden Gate Assembly of <a href="#BB1_26" class="bgscroll"> BB1_26</a>, <a href="#BB2_23" class="bgscroll"> BB2_23</a>, BB3_02 (unsuccessful), <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_16" class="bgscroll"> BB3_16</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, BB3_28 (unsuccessful), <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a></br>
 
<br>• Golden Gate Assembly of <a href="#BB1_26" class="bgscroll"> BB1_26</a>, <a href="#BB2_23" class="bgscroll"> BB2_23</a>, BB3_02 (unsuccessful), <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_16" class="bgscroll"> BB3_16</a>, <a href="#BB3_17" class="bgscroll"> BB3_17</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a>, BB3_28 (unsuccessful), <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a></br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Overnight cultures of <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a> tested for temperature sensitivity</br>
+
<br>• Overnight cultures of <a href="#C-PPP_04" class="bgscroll"> C-PPP_04</a> were tested for temperature sensitivity.</br>
 
<br>• Sent for Sequencing: <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a></br>
 
<br>• Sent for Sequencing: <a href="#C-PPP_04" class="bgscroll"> C-PPP_05</a>, <a href="#C-PPP_04" class="bgscroll"> C-PPP_06</a>, <a href="#BB3_20" class="bgscroll"> BB3_20</a>, <a href="#BB3_22" class="bgscroll"> BB3_22</a></br>
  
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<div id="8" class="yolo" style="display:none;">
 
<div id="8" class="yolo" style="display:none;">
 
<p>
 
<p>
<br>• PCR: DH5-α pSIM5, #71, #72</br>
+
<br>• PCR: #71, #72</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to protocol.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to protocol.
 
<br><br>• Golden Gate Assembly of <a href="#BB1_27" class="bgscroll"> BB1_27</a>, BB3_01 (unsuccessful), <a href="#BB3_03" class="bgscroll"> BB3_03</a>, <a href="#BB3_30" class="bgscroll"> BB3_30</a>, <a href="#BB3_31" class="bgscroll"> BB3_31</a>, <a href="#BB3_32" class="bgscroll"> BB3_32</a>, <a href="#BB3_33" class="bgscroll"> BB3_33</a></br>
 
<br><br>• Golden Gate Assembly of <a href="#BB1_27" class="bgscroll"> BB1_27</a>, BB3_01 (unsuccessful), <a href="#BB3_03" class="bgscroll"> BB3_03</a>, <a href="#BB3_30" class="bgscroll"> BB3_30</a>, <a href="#BB3_31" class="bgscroll"> BB3_31</a>, <a href="#BB3_32" class="bgscroll"> BB3_32</a>, <a href="#BB3_33" class="bgscroll"> BB3_33</a></br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Transformation of <a href="#BB3_32" class="bgscroll"> BB3_32</a> and <a href="#BB3_31" class="bgscroll"> BB3_31</a> into yeast cells</br>
 
<br>• Transformation of <a href="#BB3_32" class="bgscroll"> BB3_32</a> and <a href="#BB3_31" class="bgscroll"> BB3_31</a> into yeast cells</br>
<br>• <a href="#BB3_31" class="bgscroll"> BB3_31</a> and <a href="#BB3_32" class="bgscroll"> BB3_32</a> cultivated in YP+G418, a yeast peptone media</br> for testing the GFP expression in a plate reader.  
+
<br>• <a href="#BB3_31" class="bgscroll"> BB3_31</a> and <a href="#BB3_32" class="bgscroll"> BB3_32</a> cultivated in YP+G418: for testing the GFP expression, measurement in a plate reader.  
  
  
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</br>
 
</br>
  
<br>•Golden Gate Assembly of BB3_01 (unsuccessful), <a href="#C-PPP_07" class="bgscroll">C-PPP_07</a>, <a href="#C-PPP_07" class="bgscroll"> C-PPP_08</a>,  <a href="#C-PPP_07" class="bgscroll"> C-PPP_09</a>, <a href="#C-PPP_10" class="bgscroll">C-PPP_10</a>, <a href="#BB3_33" class="bgscroll">BB3_33</a></br>
+
<br>• Golden Gate Assembly of BB3_01 (unsuccessful), <a href="#C-PPP_07" class="bgscroll">C-PPP_07</a>, <a href="#C-PPP_07" class="bgscroll"> C-PPP_08</a>,  <a href="#C-PPP_07" class="bgscroll"> C-PPP_09</a>, <a href="#C-PPP_10" class="bgscroll">C-PPP_10</a>, <a href="#BB3_33" class="bgscroll">BB3_33</a></br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
  
<br>•Minipreparation for plasmid DNA extraction: <a href="#BB3_12" class="bgscroll">BB3_12</a>, <a href="#BB3_30" class="bgscroll">BB3_30</a>, <a href="#BB3_15" class="bgscroll">BB3_15</a></br>
+
<br>• Minipreparation for plasmid DNA extraction: <a href="#BB3_12" class="bgscroll">BB3_12</a>, <a href="#BB3_30" class="bgscroll">BB3_30</a>, <a href="#BB3_15" class="bgscroll">BB3_15</a></br>
<br>•Transformation: pSIM9</br>
+
<br>• Transformation of pSIM9</br>
  
 
<div id="BB3_35" class="modalDialog ">
 
<div id="BB3_35" class="modalDialog ">
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<br>• PCR: #88, #89, #90</br>
 
<br>• PCR: #88, #89, #90</br>
  
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of  <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_34" class="bgscroll"> BB3_34</a>, <a href="#BB3_35 Speziale" class="bgscroll"> BB3_35 Speziale</a>, <a href="#BB3_35" class="bgscroll"> BB3_35</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
 
<br><br>• Golden Gate Assembly of  <a href="#BB3_12" class="bgscroll"> BB3_12</a>, <a href="#BB3_34" class="bgscroll"> BB3_34</a>, <a href="#BB3_35 Speziale" class="bgscroll"> BB3_35 Speziale</a>, <a href="#BB3_35" class="bgscroll"> BB3_35</a>, <a href="#BB3_36" class="bgscroll"> BB3_36</a>, <a href="#BB3_37" class="bgscroll"> BB3_37</a>, <a href="#BB3_38" class="bgscroll"> BB3_38</a>, <a href="#C-PPP_10" class="bgscroll"> C-PPP_12</a></br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
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<p>
 
<p>
 
<br>• PCR: #102, #103, #105, #106</br>
 
<br>• PCR: #102, #103, #105, #106</br>
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification
+
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly of <a href="#BB2_56" class="bgscroll">BB2_56</a>, <a href="#BB3_09" class="bgscroll"> BB3_09</a>, <a href="#BB3_42" class="bgscroll"> BB3_42</a>, C-PPP_15 (unsuccessful), <a href="#C-PPP_25" class="bgscroll"> C-PPP_26</a>, C-PPP_27 (unsuccessful), <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 
<br><br>• Golden Gate Assembly of <a href="#BB2_56" class="bgscroll">BB2_56</a>, <a href="#BB3_09" class="bgscroll"> BB3_09</a>, <a href="#BB3_42" class="bgscroll"> BB3_42</a>, C-PPP_15 (unsuccessful), <a href="#C-PPP_25" class="bgscroll"> C-PPP_26</a>, C-PPP_27 (unsuccessful), <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Yeast S288C made electro-competent</br>
+
<br>• Yeast S288C were made electro-competent.</br>
<br>• Toulouse Collaboration: for further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
+
<br>• Toulouse Collaboration: For further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
  
 
<div id="BB2_56" class="modalDialog ">
 
<div id="BB2_56" class="modalDialog ">
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<br><br>• Golden Gate Assembly of <a href="#BB1_32" class="bgscroll"> BB1_32</a>, <a href="#BB2_51" class="bgscroll"> BB2_51</a>, <a href="#BB2_52" class="bgscroll"> BB2_52</a>, <a href="#BB2_53" class="bgscroll"> BB2_53</a>, <a href="#BB2_54" class="bgscroll"> BB2_54</a>, <a href="#BB2_55" class="bgscroll"> BB2_55</a>, <a href="#BB2_57" class="bgscroll"> BB2_57</a>, BB3_41 (unsuccessful), <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#BB3_45" class="bgscroll"> BB3_45</a>, <a href="#BB3_46" class="bgscroll"> BB3_46</a>, <a href="#BB3_47" class="bgscroll"> BB3_47</a>, <a href="#BB3_48" class="bgscroll"> BB3_48</a>, <a href="#BB3_49" class="bgscroll"> BB3_49</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_14</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_15</a>, <a href="#C-PPP_16" class="bgscroll"> C-PPP_16</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_19</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_20</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_21</a>, <a href="#C-PPP_22" class="bgscroll"> C-PPP_22</a>, <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 
<br><br>• Golden Gate Assembly of <a href="#BB1_32" class="bgscroll"> BB1_32</a>, <a href="#BB2_51" class="bgscroll"> BB2_51</a>, <a href="#BB2_52" class="bgscroll"> BB2_52</a>, <a href="#BB2_53" class="bgscroll"> BB2_53</a>, <a href="#BB2_54" class="bgscroll"> BB2_54</a>, <a href="#BB2_55" class="bgscroll"> BB2_55</a>, <a href="#BB2_57" class="bgscroll"> BB2_57</a>, BB3_41 (unsuccessful), <a href="#BB3_43" class="bgscroll"> BB3_43</a>, <a href="#BB3_45" class="bgscroll"> BB3_45</a>, <a href="#BB3_46" class="bgscroll"> BB3_46</a>, <a href="#BB3_47" class="bgscroll"> BB3_47</a>, <a href="#BB3_48" class="bgscroll"> BB3_48</a>, <a href="#BB3_49" class="bgscroll"> BB3_49</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_14</a>, <a href="#C-PPP_13" class="bgscroll"> C-PPP_15</a>, <a href="#C-PPP_16" class="bgscroll"> C-PPP_16</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_19</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_20</a>, <a href="#C-PPP_19" class="bgscroll"> C-PPP_21</a>, <a href="#C-PPP_22" class="bgscroll"> C-PPP_22</a>, <a href="#C-PPP_28" class="bgscroll"> C-PPP_28</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_31</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_31" class="bgscroll"> C-PPP_33</a>, <a href="#C-PPP_34" class="bgscroll"> C-PPP_34</a></br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products were then transformed into competent cells. Colonies with specific antibiotic resistances were selected by using antibiotic-containing media. The next day, positive clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
<br>• Heidelberg Collaboration: for further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
+
<br>• Heidelberg Collaboration: For further information, please visit our <a href="https://2017.igem.org/Team:BOKU-Vienna/Collaborations" target=_"blank">Collaboration</a> site.</br>
  
 
<div id="BB1_32" class="modalDialog ">
 
<div id="BB1_32" class="modalDialog ">
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<p>
 
<p>
 
<br>• Lethality Test of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
 
<br>• Lethality Test of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a></br>
<br>• Transformation of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> into <i>E.coli</i> with pSIM9</br>
+
<br>• RT activity assay </br>
Positive clones were made electro-competent and the lambda-red system was inducted. </br>
+
<br>• Lethality Test of C-PPP 24 and 30 </br>
 +
<br>• Transformation of <a href="#C-PPP_37" class="bgscroll"> C-PPP_38</a>, <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> into <i>E.coli</i> with pSIM9.</br>
 +
Positive clones were made electro-competent and the lambda-red system was induced. </br>
 
<br>• Integration: GFPuv into <i>E. coli</i> DH10B using <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> and pSIM9</br>
 
<br>• Integration: GFPuv into <i>E. coli</i> DH10B using <a href="#C-PPP_37" class="bgscroll"> C-PPP_39</a> and pSIM9</br>
 
<div id="C-PPP_37" class="modalDialog ">
 
<div id="C-PPP_37" class="modalDialog ">

Latest revision as of 20:56, 1 November 2017

Notebook

V

Notebook.


All protocols we used for the methods can be found here: Protocol

For further information regarding our Golden Gate Assembly products, we refer to our part collection site. The list of our primers can be found here.

Week 1 (03/07-07/07)
Week 2 (10/07-14/07)
Week 3 (17/07-21/07)
Week 4 (24/07-28/07)
Week 5 (31/07-04/08)
Week 6 (07/08-11/08)
Week 7 (14/08-18/08)
Week 8 (21/08-25/08)
Week 9 (28/08-01/09)
Week 10 (04/09-08/09)
Week 11 (11/09-15/09)
Week 12 (18/09-22/09)
Week 13 (25/09-29/09)
Week 14 (02/10-06/10)
Week 15 (09/10-13/10)