Line 698: | Line 698: | ||
<br>• PCR: DH5-α pSIM5, #71, #72</br> | <br>• PCR: DH5-α pSIM5, #71, #72</br> | ||
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to protocol. | Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to protocol. | ||
− | <br><br>• Golden Gate Assembly of BB1_27, BB3_01, BB3_03, BB3_30, BB3_31, BB3_32, BB3_33</br> | + | <br><br>• Golden Gate Assembly of <a href="#BB1_27" class="bgscroll"> BB1_27</a>, <a href="#BB3_01" class="bgscroll"> BB3_01</a>, <a href="#BB3_03" class="bgscroll"> BB3_03</a>, <a href="#BB3_30" class="bgscroll"> BB3_30</a>, <a href="#BB3_31" class="bgscroll"> BB3_31</a>, <a href="#BB3_32" class="bgscroll"> BB3_32</a>, <a href="#BB3_33" class="bgscroll"> BB3_33</a></br> |
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br> | GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via an OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br> | ||
<br>• BB3_32 cultivated in YP-G418, a yeast peptone media</br> | <br>• BB3_32 cultivated in YP-G418, a yeast peptone media</br> | ||
<br>• Transformation of pSIM5 and BB3_31 into yeast cells</br> | <br>• Transformation of pSIM5 and BB3_31 into yeast cells</br> | ||
+ | |||
+ | <div id="BB1_27" class="modalDialog "> | ||
+ | <div><a href="#close" title="Close" class="close">X</a> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/b8/T--BOKU-Vienna--BB127.png" style="width:100%;height:auto;"/> | ||
+ | </div></div> | ||
+ | <div id="BB3_01" class="modalDialog "> | ||
+ | <div><a href="#close" title="Close" class="close">X</a> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/4f/T--BOKU-Vienna--BB301.png" style="width:100%;height:auto;"/> | ||
+ | </div></div> | ||
+ | <div id="BB3_03" class="modalDialog "> | ||
+ | <div><a href="#close" title="Close" class="close">X</a> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/3/39/T--BOKU-Vienna--BB303.png" style="width:100%;height:auto;"/> | ||
+ | </div></div> | ||
+ | <div id="BB3_30" class="modalDialog "> | ||
+ | <div><a href="#close" title="Close" class="close">X</a> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/93/T--BOKU-Vienna--BB330.png" style="width:100%;height:auto;"/> | ||
+ | </div></div> | ||
+ | <div id="BB3_31" class="modalDialog "> | ||
+ | <div><a href="#close" title="Close" class="close">X</a> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/69/T--BOKU-Vienna--BB331.png" style="width:100%;height:auto;"/> | ||
+ | </div></div> | ||
+ | <div id="BB3_32" class="modalDialog "> | ||
+ | <div><a href="#close" title="Close" class="close">X</a> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/0a/T--BOKU-Vienna--BB332.png" style="width:100%;height:auto;"/> | ||
+ | </div></div> | ||
+ | <div id="BB3_33" class="modalDialog "> | ||
+ | <div><a href="#close" title="Close" class="close">X</a> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/1/1b/T--BOKU-Vienna--BB333.png" style="width:100%;height:auto;"/> | ||
+ | </div></div> | ||
</p></div> | </p></div> | ||
Revision as of 14:27, 31 October 2017
Notebook.
All protocols we used for the methods can be found here:
Protocol
For further information regarding our Golden Gate Assembly products, please refer to our part collection site.
Week 1 (03/07-07/07)
Week 2 (10/07-14/07)
Week 3 (17/07-21/07)
Week 4 (24/07-28/07)
Week 5 (31/07-04/08)
Week 6 (07/08-11/08)
Week 7 (14/08-18/08)
Week 8 (21/08-25/08)
Week 9 (28/08-01/09)
Week 10 (04/09-08/09)
Week 11 (11/09-15/09)
Week 12 (18/09-22/09)
Week 13 (25/09-29/09)
Week 14 (02/10-06/10)
Week 15 (09/10-13/10)