Today we began our labwork by preparing some growth media and autoclaving them. Please visit our Protocol website to view the exact procedures. We then continued planning our experiments which also meant designing some primers.
Tuesday, July 4th.
Amidst of all the planning and primer designing, we also prepared our E. Coli DH10B cells by making them chemically competent. Instructions can be found here.
Wednesday, July 5th.
• Project:
• Interlab challenge: Transformation of the given plasmids into competent cells according to our protocol.
Thursday, July 6th.
• Project:
• Interlab challenge: Measurement of OD600 over a period of 6 hours according to protocols which can be found here.