Team:BOKU-Vienna/Protocol

Protocol

Protocols

Contents

• Materials
  • LB medium - E. Coli
  • LB Agar - E. Coli
• Methods
  • Miniprep
  • Transformation of chemically competent E. Coli
  • Preparation of electro-competent Pichia pastoris cells
  • InterLab Study
  • Q5 PCR
  • Golden Gate
  • OneTaq Colony PCR

Materials

LB medium - E. Coli

Name	Amount
Hy Soy Peptone	10.0g
Yeast Extract	5.0g
NaCl	5.0g
RO-water	1000 mL

Methods

Under construction...