Difference between revisions of "Team:Bordeaux/Description"

 
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<h1>Description</h1>
 
<h1>Description</h1>
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The alternative splicing of this gene is regulated by the SUP-12's RRM (RNA Recognition Motif) domain, a region of the protein which binds to the unc-60 pre-RNA, to induce, with ASD-2, the muscular isoform. Thus the cell becomes a muscular cell. The "Stargate WCC" project aims to control alternative splicing of unc-60 by SUP-12 protein. For this purpose, our team uses a photo-inducible system, the White Collar system which exists naturally in <i>N. crassa</i> in which it regulates the circadian rhythm according to the light level. Indeed, two proteins, White Collar 1 and 2, fit together in order to form a complex : the White Collar Complex or WCC, activated by a conformational change in response to light. Then this WCC modified and activated binds to circadian genes promoters in the aim of inducing FRQ proteins. In the worm, the WCC will be implied into neural cell to activate SUP-12 protein, regulate the alternative splicing of unc-60 and generate a muscle cell instead of a neural cell.</p>
 
The alternative splicing of this gene is regulated by the SUP-12's RRM (RNA Recognition Motif) domain, a region of the protein which binds to the unc-60 pre-RNA, to induce, with ASD-2, the muscular isoform. Thus the cell becomes a muscular cell. The "Stargate WCC" project aims to control alternative splicing of unc-60 by SUP-12 protein. For this purpose, our team uses a photo-inducible system, the White Collar system which exists naturally in <i>N. crassa</i> in which it regulates the circadian rhythm according to the light level. Indeed, two proteins, White Collar 1 and 2, fit together in order to form a complex : the White Collar Complex or WCC, activated by a conformational change in response to light. Then this WCC modified and activated binds to circadian genes promoters in the aim of inducing FRQ proteins. In the worm, the WCC will be implied into neural cell to activate SUP-12 protein, regulate the alternative splicing of unc-60 and generate a muscle cell instead of a neural cell.</p>
  
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Alternatively, Q system can be used. Indeed this is a system whose repressible binary expression system was established in Drosophila and mammalian cells based on regulatory genes from <i>N. crassa</i>. This system uses a transcriptional activator QF which binds on the QUAS promoter to induce the expression of target gene. Activation by QF can be suppressed by a transcriptional repressor called QS which binds on QF. Also, suppresion of QS activity can be realised by the nontoxic small molecule quinic acid which interfere QS and QF interaction. It can be difficult to “turn off” or “turn down” gene expression by direct promoter fusion while QS expression and quinic acid effect can be an efficient approach to inhibate or activate gene expression. On the contrary, with the regulator role of quinic acid, the Q system can “turn on” gene expression at any desired time point and combines spatial and temporal control.</p>
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In conclusion, the "Stargate WCC" project is intended to control alternative splicing via different regulating mechanisms involving a logical gate that inspired us the project name. For this purpose, our team decided to focus on gene expression in muscular cell in order to control cell differentiation. This experimental procedure strives to give necessary tools in biomedical research especially in the regeneration of members. In fact, this tool could be used in research connected to stem cells therapy, Bioprinting or research in genetic diseases like Duchenne muscular dystrophy or amyotrophic lateral sclerosis. Therefore, multiple prospects open up to "Stargate WCC" project.</p>
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In conclusion, the "Stargate WCC" project is intended to control alternative splicing via different regulating mechanisms involving a logical gate that inspired us the project name. For this purpose, our team decided to focus on gene expression in muscular cell in order to control cell differentiation. This experimental procedure strives to give necessary tools to lead then some biomedical research linked to this phenomenon.</p>
 
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<h5>References</h5>
 
<h5>References</h5>
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<cite style=" color: #E0E0E0">Ohno G, Ono K, Togo M, Watanabe Y, Ono S, et al. (2012) Muscle-Specific Splicing Factors ASD-2 and SUP-12 Cooperatively Switch Alternative Pre-mRNA Processing Patterns of the ADF/Cofilin Gene in Caenorhabditis elegans. PLOS Genetics 8(10): e1002991. https://doi.org/10.1371/journal.pgen.1002991</cite>
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<cite style="color:#E0E0E0">Anyanful A, Ono K, Johnsen RC, et al. The RNA-binding protein SUP-12 controls muscle-specific splicing of the ADF/cofilin pre-mRNA in C. elegans. The Journal of Cell Biology. 2004;167(4):639-647. doi:10.1083/jcb.200407085.</cite>
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<cite style="color:#E0E0E0">Ballario P, Vittorioso P, Magrelli A, Talora C, Cabibbo A, Macino G. White collar-1, a central regulator of blue light responses in Neurospora, is a zinc finger protein. The EMBO Journal. 1996;15(7):1650-1657.</cite>
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<cite style="color:#E0E0E0">San-Miguel A, Lu H. Microfluidics as a tool for C. elegans research. In: WormBook: The Online Review of C. elegans Biology [Internet]. Pasadena (CA): WormBook; 2005-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK174829/</cite>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<h1 style="text-align:center;color:#d8b700">How to find us ?</h1>
 
<h1 style="text-align:center;color:#d8b700">How to find us ?</h1>
  
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<p style="font-size:1.5em; text-align:center; color:white">Feel free to email us to provide some feedback on our project, have some information on the team and our work, or to just say hello !</p>
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<p style="font-size:1.5em; text-align:center; color:#E0E0E0">Feel free to email us to provide some feedback on our project, have some information on the team and our work, or to just say hello !</p>
  
 
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Latest revision as of 02:31, 2 November 2017

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