This year, IGEM bordeaux aims to study the alternative splicing of the unc-60 gene in C.elegans.
In order to control the alternative splicing of this gene, it is important to control the synthesis of proteins involved in it’’s splicing. On the one hand, we want to make a photo-inducible system that will use on the two promoters of regulatory proteins ASD2 and SUP12. On the other hand, we aim to use another system called the Q system allowing more precise control of the regulatory proteins of the unc-60 gene. We also aim to create a microfluidic system to observe the results of these genetic modifications on C. elegans.