Difference between revisions of "Team:Bordeaux/Results"

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<h1>Results</h1>
 
  
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<p>Here you can describe the results of your project and your future plans. </p>
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<div class="title" style="margin-top:-30px" >Results</div>
  
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<h5>What should this page contain?</h5>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
 
  
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h2>During our lab work, we managed to do 3 constructions:</h2>
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<p>
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pmyo2::GFP
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pmyo3::unc-60::RFP
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pmyo3::unc60::GFP
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These 3 constructions xere microinjected in worms under the microscope.
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Pmyo2::GFP is used as a brenchmark in order to know if our worms were well injected. The promoter pmyo2 is tissue specific, it allows the expression of the gene myo-2 in the pharynx of the C.elegans. Thanks to the green fluorescent pharynx, we can screen transformed worms from non transformed worms.
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The two others constructions, pmyo3::unc-60::RFP and pmyo3::unc60::GFP, will allow us to see how the two different isoforms unc-60A (RFP) and unc-60B (GFP) are expressed in the body wall muscle.
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Here are a few pictures about what we obtained under the microscope.</p>
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<img style="width:400px" src="https://static.igem.org/mediawiki/2017/f/f6/T--Bordeaux--celegansGFP.png" alt="feed worm GFP"><figcaption>Fed worm GFP</figcaption>
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<img style="width:400px" src="https://static.igem.org/mediawiki/2017/8/8a/T--Bordeaux--celegansRFP.png" alt="feed worm GFP"><figcaption>Fed worm RFP</figcaption>
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<img style="width:400px" src="https://static.igem.org/mediawiki/2017/f/f6/T--Bordeaux--celegansGFP.png" alt="starve worm GFP"><figcaption>Starving worm GFP</figcaption>
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<img style="width:400px" src="https://static.igem.org/mediawiki/2017/5/5c/Bordeaux2017_CelegansStarve2RFP.jpeg" alt="starve worm RFP"><figcaption>Starving worm RFP</figcaption>
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<p>
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We observed the worms under two conditions: starved and non-starved worms. C.elegans eats the bacteria and fungi. When there are no more bacteria on the petri dish, the worms are starved. It can be useful to see if the starvation can change a phenotype, as the worm can be adaptive.</p>
  
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<h5> Project Achievements </h5>
 
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
  
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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      <div style="padding:7px; background-color:#000106;padding-bottom:20px;margin-bottom:-30px;">
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<h1 style="text-align:center;color:#d8b700">How to find us ?</h1>
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<p style="font-size:1.5em; text-align:center; color:#E0E0E0">Feel free to email us to provide some feedback on our project, have some information on the team and our work, or to just say hello !</p>
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  font-size: 2em;
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  ">
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  <a href="https://www.facebook.com/BordeauxIGEM" target="_blank">
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      Facebook</a> </li>
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  <a href="https://twitter.com/bordeauxigem" target="_blank">
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      Twitter</a></li>
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  <a href="https://igembordeaux.wordpress.com/" target="_blank">
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      Wordpress</a></li>
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</ul>
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<h5 style="float:left ; color:#E0E0E0">Mail:
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  <a style="font-family: 'Arial', cursive; font-size: 1.5em;" href="mailto:igembdx@gmail.com">igembdx@gmail.com</a></h5>
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<h5 style="text-align:right ; color:#E0E0E0"><i>Copyright &#169; iGEM Bordeaux 2017</i></h5>
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Latest revision as of 23:28, 26 November 2017

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