Difference between revisions of "Team:BostonU/Parts"

 
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  <p class="inline-heading-type mainwrap">Composite: toeholdFE1</p>
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<p class="inline-heading-type mainwrap">We submitted two composite parts to the registry: BBa_K2411005 and BBa_K2411008.</p>
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  <p class="body-type mainwrap">Composite part consisting of deGFP under the control of the first toehold switch we characterized in our cell-free system, the best performer from the switches developed by <a href="#">Green et al.</a> Also includes our bacterial promoter and terminator parts.</p>
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<p class="body-type mainwrap">FIG </p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411005" style="text-indent:0pt;">BBa_K2411005</a>: Forward Engineered Toehold 1 with deGFP gene</p>
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<p class="body-type mainwrap">This device encodes for a toehold that when activated expresses deGFP. It has an OR2-OR1 promoter, followed by the first forward engineered toehold sequence designed by Green et al. The deGFP follows the toehold, and the device ends with a T7 terminator. The part has been designed for and tested in an E. coli derived transcriptional translation cell free system. In order for the part to function, it needs to be used in conjunction with a trigger RNA. Characterization data can be view on our results page or on the registry.</p>
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<img src="https://static.igem.org/mediawiki/2017/1/1b/T--BostonU--Part_th2comp.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
 
<p class="body-type mainwrap">&nbsp;</p>
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<p class="inline-heading-type mainwrap">Composite toeholdFE2</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411008" style="text-indent:0pt;">BBa_K2411008</a>: Forward Engineered Toehold 2 with deGFP gene</p>
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<p class="body-type mainwrap">Composite part consisting of deGFP under the control of the second toehold switch we characterized in our cell-free system, the second best performer from the switches developed by <a href="#">Green et al.</a> Also includes our bacterial promoter and terminator parts.</p>
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<p class="body-type mainwrap">This device encodes for a toehold that when activated expresses deGFP. It has an OR2-OR1 promoter, followed by the second best forward engineered toehold sequence designed by Green et al. The deGFP follows the toehold, and the device ends with a T7 terminator. The part has been designed for and tested in an E. coli derived transcriptional translation cell free system. In order for the part to function, it needs to be used in conjunction with a trigger RNA. Characterization data can be view on our results page or on the registry.&nbsp;</p>
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<p class="body-type mainwrap">In addition to these composite parts, we created registry pages for the OR2-OR1 promoter, the T500 terminator, and both toehold architectures that we used in our composite parts.</p>
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<img src="https://static.igem.org/mediawiki/2017/0/08/T--BostonU--Part_th1comp.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
 
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<p class="body-type mainwrap">FIG </p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411000" style="text-indent:0pt;">BBa_K2411000</a>: OR2-OR1 Promoter</p>
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<p class="body-type mainwrap">This is a constitutively active promoter that can be used in E. coli and E. coli derived transcription translation systems. This part is derived from the lambda bacteriaphage.</p>
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<img src="https://static.igem.org/mediawiki/2017/1/1f/T--BostonU--Part_or2or1.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
 
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<p class="inline-heading-type mainwrap">OR2-OR1 Bacterial Promoter</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411002" style="text-indent:0pt;">BBa_K2411002</a>: T500 Terminator</p>
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<p class="body-type mainwrap">Promoter used in constructs designed for our cell-free system.</p>
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<p class="body-type mainwrap">This is a bacterial terminator.</p>
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<img src="https://static.igem.org/mediawiki/2017/4/42/T--BostonU--Part_t500.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
 
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<p class="body-type mainwrap">FIG </p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411004" style="text-indent:0pt;">BBa_K2411004</a>: Toehold 1</p>
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<p class="body-type mainwrap">This sequence is the forward engineered toehold from Green et. al. This part contains the hairpin loop sequence that with the RBS. There is no gene associated with this toehold.</p>
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<center>
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<img src="https://static.igem.org/mediawiki/2017/c/c8/T--BostonU--Part_th2.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
 
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<p class="inline-heading-type mainwrap">T500 Bacterial Terminator</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411007" style="text-indent:0pt;">BBa_K2411007</a>: Toehold 2</p>
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<p class="body-type mainwrap">Terminator used in constructs designed for our cell-free system. Absolutely a reference to the movie.</p>
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<p class="body-type mainwrap">This sequence is the second best performing forward engineered toehold from Green et. al. There is no gene associated with this toehold.</p>
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<center>
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<img src="https://static.igem.org/mediawiki/2017/7/74/T--BostonU--Part_th1.png" width = 25%></img>
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<p class="body-type mainwrap">FIG </p>
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<p class="body-type mainwrap">In addition, the deGFP part utilized in our composite part was submitted under BBa_K2205001 by iGEM17_Newcastle.</p>
 
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<p class="inline-heading-type mainwrap">toeholdFE1</p>
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<p class="body-type mainwrap">The sequences for the promoter, terminator, and deGFP were obtained from the pBEST plasmid used to Vincent Noireaux and his lab with their cell-free system [1].</p>
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<p class="body-type mainwrap">A toehold switch part including a ribosomal binding site and start codon.</p>
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<p class="body-type mainwrap">&nbsp;</p>
 
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<p class="body-type mainwrap">FIG </p>
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<p class="body-type mainwrap">The sequences for the toeholds were obtained from Green et al. [2].</p>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="inline-heading-type mainwrap">toeholdFE2 </p>
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<p class="body-type mainwrap">A toehold switch part including a ribosomal binding site and a start codon.</p>
 
<p class="body-type mainwrap">A toehold switch part including a ribosomal binding site and a start codon.</p>
 
<p class="body-type mainwrap">&nbsp;</p>
 
<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap">FIG </p>
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<p class="body-type mainwrap">[1]Shin, Jonghyeon, and Vincent Noireaux. "Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70." Journal of biological engineering 4.1 (2010): 8.</p>
 
<p class="body-type mainwrap">&nbsp;</p>
 
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<p class="body-type mainwrap">[2] Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.</p>
 
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Latest revision as of 06:16, 31 October 2017

OUR PARTS