Difference between revisions of "Team:Bristol/Safety"
| Line 9: | Line 9: | ||
<div class="banner-text-sm"> | <div class="banner-text-sm"> | ||
<h1 class="Up">Safety</h1> | <h1 class="Up">Safety</h1> | ||
| − | <p class="Up">In | + | <p class="Up">"In case of fire, exit building before running gels."</p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 19: | Line 19: | ||
<hr class="featurette-divider"> | <hr class="featurette-divider"> | ||
| − | + | <div class="row align-items-center fadeIn"> | |
| − | + | <div class="col-md-7 Up"> | |
| − | + | <h2 class="featurette-heading">Safety features of our project design</h2> | |
| − | + | <p class="lead"> | |
| − | + | The real world implementation of our BREATHE pods provides a unique situation where the general public will be walking next to cultures of E. coli during their day to day activities. The street-side location of our pods makes them particularly vulnerable | |
| − | + | to damage, from vandalism, traffic incidents and extreme weather to name a few examples. It is therefore our responsibility to reduce the risk of the general public coming into contact with the E. coli inside our pods, and to reduce the harm | |
| − | + | done if this does occur. | |
| − | + | </p> | |
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| + | <div class="col-md-5 Pop"> | ||
| + | <img class="featurette-image img-responsive center-block" src="https://static.igem.org/mediawiki/2017/5/50/T--Bristol--Pods.png"> | ||
| + | </div> | ||
| + | </div> | ||
| − | <div class="col-md- | + | <div class="row align-items-center fadeIn"> |
| + | <div class="col-md-12 Up"> | ||
<p class="lead"> | <p class="lead"> | ||
| − | + | Using lab-dependent Top10 cells provides a primary level of protection. | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
| − | <!-- <div class="col-md-12 Pop"> | + | </div> |
| + | |||
| + | <div class="col-md-12 Right notebook"> | ||
| + | <p class="lead"> | ||
| + | <br>Our pods should: | ||
| + | </p> | ||
| + | <p class="lead"> | ||
| + | <ul class="list-group"> | ||
| + | <li class="list-group-item">• Be leak proof</li> | ||
| + | <li class="list-group-item">• Be shatter-proof</li> | ||
| + | <li class="list-group-item">• Be impact-resistant</li> | ||
| + | <li class="list-group-item">• Have nearby hand sanitizer dispensers</li> | ||
| + | <li class="list-group-item">• Be high up to reduce risk of impact (where this would not severely impair functionality)</li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | </div> | ||
| + | <!-- <div class="col-md-12 Pop"> | ||
<h3>[image title]</h3> | <h3>[image title]</h3> | ||
<img src=""> | <img src=""> | ||
| Line 52: | Line 61: | ||
| − | + | <hr class="featurette-divider"> | |
| − | + | <div class="row parts align-items-center fadeIn"> | |
| − | + | <div class="col-md-12 Up"> | |
| − | + | <h2 class="featurette-heading">Safety in the lab</h2> | |
| − | + | </div> | |
| − | + | <div class="col-md-12 Right notebook"> | |
| − | + | <p class="lead"> | |
| − | + | The following safety procedures were followed in the lab: | |
| − | + | </p> | |
| − | + | <ul class="list-group lead"> | |
| − | + | <li class="list-group-item">• Ethidium bromide was handled only when wearing gloves. All ethidium bromide waste was disposed of in sealed containers or in specific bins.</li> | |
| − | + | <li class="list-group-item">• Any glassware which touched ethidium bromide was rinsed, and the water detoxified.</li> | |
| − | + | <li class="list-group-item">• Suitable PPE was worn at all times when handling molecular biology reagents: lab coat, gloves, closed shoes.</li> | |
| − | + | <li class="list-group-item">• All work handling E. coli was conducted using aseptic technique.</li> | |
| − | + | <li class="list-group-item">• Gel extraction for purification of PCR products was conducted in a dark room, wearing UV resistant eye protection and with all skin covered.</li> | |
| − | + | </ul> | |
| − | + | </div> | |
| − | + | <!-- <div class="col-md-12 Pop"> | |
<h3>[image title]</h3> | <h3>[image title]</h3> | ||
<img src=""> | <img src=""> | ||
</div> --> | </div> --> | ||
| − | |||
| − | |||
</div> | </div> | ||
| + | |||
| + | </div> | ||
| − | + | <!-- <div class="row parts align-items-center fadeIn"> | |
<div class="col-md-12 Up"> | <div class="col-md-12 Up"> | ||
<h2>Safe shipment</h2> | <h2>Safe shipment</h2> | ||
Revision as of 19:02, 1 November 2017
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
Safety features of our project design
The real world implementation of our BREATHE pods provides a unique situation where the general public will be walking next to cultures of E. coli during their day to day activities. The street-side location of our pods makes them particularly vulnerable to damage, from vandalism, traffic incidents and extreme weather to name a few examples. It is therefore our responsibility to reduce the risk of the general public coming into contact with the E. coli inside our pods, and to reduce the harm done if this does occur.
Using lab-dependent Top10 cells provides a primary level of protection.
Our pods should:
- • Be leak proof
- • Be shatter-proof
- • Be impact-resistant
- • Have nearby hand sanitizer dispensers
- • Be high up to reduce risk of impact (where this would not severely impair functionality)
Safety in the lab
The following safety procedures were followed in the lab:
- • Ethidium bromide was handled only when wearing gloves. All ethidium bromide waste was disposed of in sealed containers or in specific bins.
- • Any glassware which touched ethidium bromide was rinsed, and the water detoxified.
- • Suitable PPE was worn at all times when handling molecular biology reagents: lab coat, gloves, closed shoes.
- • All work handling E. coli was conducted using aseptic technique.
- • Gel extraction for purification of PCR products was conducted in a dark room, wearing UV resistant eye protection and with all skin covered.
