Difference between revisions of "Team:CGU Taiwan/HP/Gold Integrated"

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Revision as of 15:28, 1 November 2017

iGem CGU_Taiwan 2017 - Collaborations


Integrated human practice

To apply our idea to paper industry, we first need a great design. For the design, we not only focus on the construction of our engineered yeast, but also care about the applicability in the future. That is to say, we want to provide a design that is usable and attractive for companies, and do good to people and our environment at the same time .


Study Enzyme Deinking From Dr. Su, Yu-Chang

To study enzyme deinking method, we have visited Dr. Su, Yu-Chang, an expert of dendrochemistry in National Chung Hsing University, Taiwan. Dr. Su had advised us several kinds of enzymes which are able to degrade cellulose and hemicellulose, and shared the experimental conditions that had been applied on enzymes in his research with us, which became a great help for the construction of our process. During the interview, we also had a tour to Dr. Su’s laboratory which equipped with small scale paper making machines. Later we established our own paper making process according to the learning from Dr. Su.


Study The Yeast Two-Hybrid System From Dr. Chang, Chuang-Rung

After knowing some candidate enzymes for deinking process, we had done molecular docking simulation to figure out which enzymes have best affinity with our substrate cellulose. Through the ranking of affinity, we had chosen three kinds of enzymes _ Glucanase, Xylanase and Lipase to deink paper, and planned to use Saccharomyces cerevisiae as our engineered host. For the purpose of preserving most fibers, we came to an idea of ‘’light stimulation’’, using light to control the secretion of enzymes. To know more about the light regulatory system in yeast, we had visited Dr. Chang, Chung-Rung in National Tsing Hua University. Dr. Chang is an expert in Epigenetics and Yeast Genetics, and he not only analyzed the advantage and disadvantage of using yeast as our host, but also shared how yeast two-hybrid system works. Dr. Chang’s precious advice had great effect on the design of our photo-induced system.


Connect With Industry_ Visiting Cheng Loong Paper Company

Cheng Loong Paper Company is one of the biggest paper company in Taiwan, so we contacted them and hoped to understand how our design can be applied in paper industry. During the visit, we had clarified three things_ First, the chemical pollution used to deink cause water pollution, and the company wanted to remove the use of chemicals due to the high cost of sewage treatment. Second, the strength of fiber is according to the length of it, therefore, reduce the opportunity of the contact between fibers and deinking agents preserve strength. Third, the cost of white paper produced by our device is higher than current method.

According to our discoveries, Cheng Loong suggested us to focus on preserving fibers. And due to low light penetration in paper pulp, we decided to deink paper before cutting it into paper pulp. Thus, we constructed a new deinking process which can specifically deink and hoped to preserver more fibers. Through the visiting, we understood the need and lack of factories, and tried to adjust our design to make it more practical for industry.


Contact 2012 TU Munich iGEM team for biobricks improvement

In the last design of our project, we contacted 2012 TU Munich to learn about their red light induced system biobricks. Through meetings, we gradually understand their original design. We appreciate 2012 TU Munich for giving us many advices of how we improve the biobricks. Lastly, we optimize the protein codon and reverse the direction of protein expression.