Team:CMUQ/BioSensor Lab R
BioSensor Lab Results
As we were unable to transform the construct, therefore, the results for Salt Sensor testing were obtained from iGEM Pitt as a part of the collaboration
1. The results for the RFP fluorescence measured for three replicated of the blank as well as two constructs at excitation 585nm/emission 607nm.
| NFkB (negative control) | 0mM | 50mM | 100mM | 200mM | 400mM | 800mM | 1600mM |
|---|---|---|---|---|---|---|---|
| 1 | 326525 | 322701 | 324569 | 324359 | 329257 | 424215 | 447835 |
| 2 | 324213 | 321232 | 323679 | 324437 | 326850 | 421070 | 436049 |
| 3 | 321979 | 321615 | 322685 | 324569 | 327046 | 399897 | 406696 |
| Wildtype | 0mM | 50mM | 100mM | 200mM | 400mM | 800mM | 1600mM |
| 1 | 322379 | 324207 | 326546 | 324794 | 330924 | 341689 | 388728 |
| 2 | 322539 | 323331 | 326077 | 324704 | 331310 | 367254 | 438918 |
| 3 | 322369 | 323430 | 326386 | 324750 | 330914 | 353266 | 381339 |
| Consensus | 0mM | 50mM | 100mM | 200mM | 400mM | 800mM | 1600mM |
| 1 | 326742 | 326016 | 325673 | 329309 | 337418 | 377574 | 378246 |
| 2 | 325087 | 326368 | 326416 | 327722 | 337804 | 367197 | 400206 |
| 3 | 325689 | 324992 | 325312 | 327109 | 334483 | 364028 | 373127 |
2. Averaging and normalizing the fluorescence readings measured for our blank and two constructs.
3. The normalized fluorescence values for the two constructs at different NaCl concentrations.
