Difference between revisions of "Team:CMUQ/Dspb Lab R"

 
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</table>
 
</table>
  
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<img src="https://static.igem.org/mediawiki/2017/b/b3/Dspb1.png" width=55%>
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<img src="https://static.igem.org/mediawiki/2017/b/b3/Dspb1.png" width=55% style="margin-left:100px;">
  
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<p style="font-size: 20px;margin-left:80px; margin-right:40px;">2. Averaging and normalizing the fluorescence readings measured from culture before pelleting the cells and then after pelleting the cells. </p>
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<table style="width:50%; margin-left:100px;">
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 +
  <tr>
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 +
    <th></th>
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    <th colspan="5">Fluorescence </th>
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 +
   
 +
 +
  </tr>
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  <tr>
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    <td>pDP12 </td>
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    <td>-0.033</td>
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    <td>0.02</td>
 +
    <td>0.036</td>
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    <td>0.007666667</td>
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    <td>0.036115555</td>
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 +
 
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  </tr>
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  <tr>
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    <td>cells t=0</td>
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    <td>-0.029</td>
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    <td>0.008</td>
 +
    <td>-0.008</td>
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    <td>-0.009666667</td>
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    <td>0.018556221</td>
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  </tr>
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  <tr>
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    <td>cells t=16</td>
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    <td>0.09</td>
 +
    <td>0.032</td>
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    <td>0.07</td>
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    <td>0.064</td>
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    <td>0.02946184</td> 
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 +
  </tr>
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  <tr>
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    <td>supernatant t=0</td>
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    <td>0</td>
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    <td>-0.145</td>
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    <td>0.0065</td>
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    <td>-0.046166667</td>
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    <td>0.085653858</td> 
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 +
  </tr>
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    <tr>
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    <td>supernatant t=16</td>
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    <td>0.048</td>
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    <td>0.122</td>
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    <td>0.045</td>
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    <td>0.071666667</td>
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    <td>0.043615746</td> 
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 +
  </tr>
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  <th></th>
 +
  <th></th>
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  <th></th>
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  <th></th>
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  <th></th>
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  <th></th>
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  <tr>
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  </tr>
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    <tr>
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    <td>Norm. cells t=0</td>
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    <td>0.004</td>
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    <td>-0.012</td>
 +
    <td>-0.044</td>
 +
    <td>-0.017333333</td>
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    <td>0.024440404</td> 
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 +
  </tr>
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 +
    <tr>
 +
    <td>Norm. cells t=16</td>
 +
    <td>0.123</td>
 +
    <td>0.012</td>
 +
    <td>0.034</td>
 +
    <td>0.056333333</td>
 +
    <td>0.058773577</td> 
 +
 +
  </tr>
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    <tr>
 +
    <td>Norm. supernatant t=0</td>
 +
    <td>0.033</td>
 +
    <td>-0.165</td>
 +
    <td>-0.0295</td>
 +
    <td>-0.053833333</td>
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    <td>0.101217999</td> 
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 +
  </tr>
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 +
    <tr>
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    <td>Norm. supernatant t=16</td>
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    <td>0.081</td>
 +
    <td>0.102</td>
 +
    <td>0.009</td>
 +
    <td>0.064</td>
 +
    <td>0.048774994</td> 
 +
 +
  </tr>
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 +
</table>
 
</div>
 
</div>
  

Latest revision as of 02:03, 2 November 2017

Dspb Lab Results

The following results were obtained for the Dispersin B, which is an improvement on an Existing part:

1. The results for the GFP fluorescence measured for three replicates of the LB (blank) as well as two constructs at excitation 585nm/emission 607nm

Average SD t test
LB -0.004 -0.0013 -0.055 -0.0201 0.030254421
t=0 supernatant -0.034 0.009 0.005 0.006666667 0.023755701 0.328585241
t=16 supernatant 0.153 0.038 0.046 0.079 0.064210591 0.050130586

2. Averaging and normalizing the fluorescence readings measured from culture before pelleting the cells and then after pelleting the cells.

Fluorescence
pDP12 -0.033 0.02 0.036 0.007666667 0.036115555
cells t=0 -0.029 0.008 -0.008 -0.009666667 0.018556221
cells t=16 0.09 0.032 0.07 0.064 0.02946184
supernatant t=0 0 -0.145 0.0065 -0.046166667 0.085653858
supernatant t=16 0.048 0.122 0.045 0.071666667 0.043615746
Norm. cells t=0 0.004 -0.012 -0.044 -0.017333333 0.024440404
Norm. cells t=16 0.123 0.012 0.034 0.056333333 0.058773577
Norm. supernatant t=0 0.033 -0.165 -0.0295 -0.053833333 0.101217999
Norm. supernatant t=16 0.081 0.102 0.009 0.064 0.048774994