Team:Calgary/BetaOxidation
Beta Oxidation
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Aim
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Volatile fatty acids & beta-oxidation pathway
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Gene construct
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Chassis and vector
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Experiment
After spinning down the culture in flasks, the cells were resuspended in 1x PBS. The OD600 readings were taken and are shown as follows:
| Condition | OD600 of replicate 1 | OD600 of replicate 2 | OD600 of replicate 3 |
|---|---|---|---|
| pET29b in BL21 (Negative control) | 2.659 | 2.001 | 2.899 |
| Glucose (Positive control) | 1.934 | 1.887 | 1.919 |
| Pure VFAs | 0.510 | 0.571 | 0.532 |
| Fermented "syn poo" supernatant | 2.533 | 2.559 | 2.349 |
