Difference between revisions of "Team:Calgary/PHB Fermentation"
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<p>The genetically engineered bacteria would be contained in the <i>stirred tank bioreactor </i>at 37 degrees Celsius and under anaerobic conditions. A continues flow of VFA rich stream is generated by the pump. The volume of the tank is 5L to account for the overflow and liquid recycling. <p> | <p>The genetically engineered bacteria would be contained in the <i>stirred tank bioreactor </i>at 37 degrees Celsius and under anaerobic conditions. A continues flow of VFA rich stream is generated by the pump. The volume of the tank is 5L to account for the overflow and liquid recycling. <p> | ||
<p>The output stream, which contains some of the bacteria, secreted PHB and unused VFAs passes through a <i>mechanical self-cleaning filter</i> (0.2 micron filter scale) to remove and recycle the bacteria back into the bioreactor. This setup was selected because it is very ESM friendly and eliminates fouling issues associated with membrane bioreactors (which was the second best option considered). The liquid output from the self-cleaning filter then flows into the final stage of the process – PHB extraction. </p> | <p>The output stream, which contains some of the bacteria, secreted PHB and unused VFAs passes through a <i>mechanical self-cleaning filter</i> (0.2 micron filter scale) to remove and recycle the bacteria back into the bioreactor. This setup was selected because it is very ESM friendly and eliminates fouling issues associated with membrane bioreactors (which was the second best option considered). The liquid output from the self-cleaning filter then flows into the final stage of the process – PHB extraction. </p> | ||
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Revision as of 00:30, 14 October 2017
PHB Fermentation
The genetically engineered bacteria would be contained in the stirred tank bioreactor at 37 degrees Celsius and under anaerobic conditions. A continues flow of VFA rich stream is generated by the pump. The volume of the tank is 5L to account for the overflow and liquid recycling.
The output stream, which contains some of the bacteria, secreted PHB and unused VFAs passes through a mechanical self-cleaning filter (0.2 micron filter scale) to remove and recycle the bacteria back into the bioreactor. This setup was selected because it is very ESM friendly and eliminates fouling issues associated with membrane bioreactors (which was the second best option considered). The liquid output from the self-cleaning filter then flows into the final stage of the process – PHB extraction.
