Difference between revisions of "Team:Calgary/Process"
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<a href="https://2017.igem.org/Team:Calgary/VFA_Fermentation"><img src="https://static.igem.org/mediawiki/2017/9/97/Calgary2017_RightArrowButton.png"></a> | <a href="https://2017.igem.org/Team:Calgary/VFA_Fermentation"><img src="https://static.igem.org/mediawiki/2017/9/97/Calgary2017_RightArrowButton.png"></a> | ||
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| + | <p> Our proposed process is shown in Figure 1. </p> | ||
| + | <p> In the <a href="https://2017.igem.org/Team:Calgary/VFA_Fermentation">first step</a> of the process, astronaut’s feces are collected into a storage tank using a vacuum toilet. Feces are then transferred into another tank and left to ferment for 3 days with bacteria naturally found in human feces to increase the concentration of volatile fatty acids (VFA) that are later consumed by engineered <i>E. coli</i> to produce PHB. <a href="https://2017.igem.org/Team:Calgary/SolidLiquidSeparation">Next</a>, the liquid containing VFA is separated from solid particles using centrifugation followed by filtration. The resulting liquid containing VFA is then passed to another storage tank. From there, VFA are added to a <a href="https://2017.igem.org/Team:Calgary/PHB_Fermentation">fermenter</a> inoculated with PHB-producing <i>E. coli</i>. Lastly, produced PHB is <a href="https://2017.igem.org/Team:Calgary/Extraction">extracted</a> from the liquid harvest stream. Produced PHB can be used in Selective Laser Sintering (SLS) 3D printer without additional processing. </p> | ||
<p><center><img src="https://static.igem.org/mediawiki/2017/1/19/Calgary2017_ProcessOverview.png" alt="Process Overview" style="width:70%"></center></p> | <p><center><img src="https://static.igem.org/mediawiki/2017/1/19/Calgary2017_ProcessOverview.png" alt="Process Overview" style="width:70%"></center></p> | ||
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| + | <h2>Applied Design</h> | ||
</html> | </html> | ||
Revision as of 20:09, 30 October 2017
Process
Our proposed process is shown in Figure 1.
In the first step of the process, astronaut’s feces are collected into a storage tank using a vacuum toilet. Feces are then transferred into another tank and left to ferment for 3 days with bacteria naturally found in human feces to increase the concentration of volatile fatty acids (VFA) that are later consumed by engineered E. coli to produce PHB. Next, the liquid containing VFA is separated from solid particles using centrifugation followed by filtration. The resulting liquid containing VFA is then passed to another storage tank. From there, VFA are added to a fermenter inoculated with PHB-producing E. coli. Lastly, produced PHB is extracted from the liquid harvest stream. Produced PHB can be used in Selective Laser Sintering (SLS) 3D printer without additional processing.
Applied Design
