Team:Calgary/Synthesis

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Synthesis

Our goal was to produce polyhydroxybutyrate (PHB) by utilizing the nutrients present in human waste. Thus, the first step of our project was to design efficient and optimal pathways that can utilize the abundant and useful nutrients present in human waste to make bioplastic. After analyzing the composition of fermented synthetic human waste, we decided to focus on two major nutrients: acetyl-coA and volatile fatty acids (VFAs). Acetyl-coA is the end product of the glycolysis pathway, which is converted to acetic acid (...% in supernatant) . Volatile fatty acids include ….. and are present in …% in fermented supernatant . Thus, by using products of glycolysis and volatile fatty acids in human waste, our pathway utilizes …% of the carbon source . Finally, in order to convert the feedstock nutrients into PHB a naturally existing pathway in R. eutropha that converts acetyl-coA to PHB and beta-oxidation pathway that converts VFAs to PHB was selected.

WORKS CITED

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