Difference between revisions of "Team:Chalmers-Gothenburg/Parts"

 
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Line 204: Line 204:
 
top: 0;
 
top: 0;
 
   width: 100%;
 
   width: 100%;
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   -webkit-touch-callout: none;
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width: cover;
 
width: cover;
 +
  min-width:1440px;
 
   text-align: center;
 
   text-align: center;
 
   margin: auto;
 
   margin: auto;
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background:#e6e6e6;   
 
background:#e6e6e6;   
−
 
 
}
 
}
  
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     text-align: left;
 
     text-align: left;
 
     text-decoration: none;
 
     text-decoration: none;
−
background: #e6e6e6;
+
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font-size:12pt !important;
 
font-size:12pt !important;
 
}
 
}
Line 678: Line 678:
 
}
 
}
  
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/*.header is the class used in the header image at the top of pages*/
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  background-repeat: repeat;
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−
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background-color:#000000;
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}
 
}
  
−
.header_Achievements_biobricks {
 
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  font-size: 45pt;
 
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position:bottom;
 
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−
/*  min-height: 500px;*/
 
  
−
  background-attachment: fixed;
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/* Banner: name of the page */
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  background-position: center;
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#banner {
−
  background-repeat: no-repeat;
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height: 100%;
−
  background-size: cover;
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        position: relative;
−
background-color:#000000;
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        bottom: 50px;
−
+
    float: left;
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
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−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
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        font-size: 80pt; /*35pt*/
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font-family:"Lato", cursive;
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line-height: 65pt;
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−
.header_Project_theory {
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#banner h3 {
−
  font-size: 45pt;
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display: block;
−
  color: black;
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position: absolute;
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  text-align: center;
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bottom: 50px;
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position:bottom;
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left: 200px;
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  background-image: url("pictures_17/backgrounds/pen_paper.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
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        font-size: 80pt; /*35pt*/
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/* min-height: 500px;*/
+
font-family:"Lato", cursive;
 +
        color:#c91a03 ;
 +
line-height: 65pt;
 +
        text-shadow: 5px 5px #000000;
 +
        /*border-style:dashed;*/
 +
padding: 20px 20px 20px 20px;
  
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
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−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
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  user-select: none;
 
−
  cursor: default;
 
 
}
 
}
  
−
.header_Project_constructs {
+
#banner h4 {
−
  font-size: 45pt;
+
display: block;
−
  color: black;
+
position: absolute;
−
  text-align: center;
+
bottom: -40px;
−
position:bottom;
+
left: 200px;
−
  background-image: url("pictures_17/backgrounds/DNA.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
+
        font-size: 40pt; /*35pt*/
−
/*  min-height: 500px;*/
+
font-family:"Lato", cursive;
−
 
+
        color:#c91a03 ;
−
  background-attachment: fixed;
+
line-height: 65pt;
−
  background-position: center;
+
        text-shadow: 3px 3px #000000;
−
  background-repeat: no-repeat;
+
        /*border-style:dashed;*/
−
  background-size: cover;
+
padding: 20px 20px 20px 20px;
−
background-color:#000000;
+
−
+
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
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−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
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  -ms-user-select: none;
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  user-select: none;
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−
  cursor: default;
+
 
}
 
}
  
−
.header_Team_students {
 
−
  font-size: 45pt;
 
−
  color: black;
 
−
  text-align: center;
 
−
position:bottom;
 
−
  background-image: url("pictures_17/team_photos_2017/group_photo_stairs_bw.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
 
−
/*  min-height: 500px;*/
 
−
 
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
−
 
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
 
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−
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−
  user-select: none;
 
−
  cursor: default;
 
−
}
 
−
 
−
.header_Team_supervisors {
 
−
  font-size: 45pt;
 
−
  color: black;
 
−
  text-align: center;
 
−
position:bottom;
 
−
  background-image: url("pictures_17/backgrounds/Chemistry_entrance_bw.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
 
−
/*  min-height: 500px;*/
 
−
 
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
−
 
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
 
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−
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−
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−
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−
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−
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−
  user-select: none;
 
−
  cursor: default;
 
−
}
 
−
 
−
.header_Team_sponsors {
 
−
  font-size: 45pt;
 
−
  color: black;
 
−
  text-align: center;
 
−
position:bottom;
 
−
  background-image: url("pictures_17/backgrounds/money_bw.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
 
−
/*  min-height: 500px;*/
 
−
 
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
−
 
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
 
−
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−
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−
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−
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−
  -webkit-user-select: none;
 
−
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−
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−
  -ms-user-select: none;
 
−
  user-select: none;
 
−
  cursor: default;
 
−
}
 
−
 
−
.header_Team_collaborations {
 
−
  font-size: 45pt;
 
−
  color: black;
 
−
  text-align: center;
 
−
position:bottom;
 
−
  background-image: url("pictures_17/backgrounds/shaking_hands_darker.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
 
−
/*  min-height: 500px;*/
 
−
 
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
−
 
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
 
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−
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−
  border-image-slice: 1;
 
−
  -webkit-touch-callout: none;
 
−
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−
  -khtml-user-select: none;
 
−
  -moz-user-select: none;
 
−
  -ms-user-select: none;
 
−
  user-select: none;
 
−
  cursor: default;
 
−
}
 
−
 
−
.header_Team_attributions {
 
−
  font-size: 45pt;
 
−
  color: black;
 
−
  text-align: center;
 
−
position:bottom;
 
−
  background-image: url("pictures_17/backgrounds/Chalmers_port_4.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
 
−
/*  min-height: 500px;*/
 
−
 
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
−
 
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
 
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−
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−
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−
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−
  -moz-user-select: none;
 
−
  -ms-user-select: none;
 
−
  user-select: none;
 
−
  cursor: default;
 
−
}
 
−
 
−
.header_Notebook_journal {
 
−
  font-size: 45pt;
 
−
  color: black;
 
−
  text-align: center;
 
−
position:bottom;
 
−
  background-image: url("pictures_17/backgrounds/Notebook.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
 
−
/*  min-height: 500px;*/
 
−
 
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
−
 
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
 
−
  -webkit-border-image: -webkit-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%)*/
 
−
  border-image: linear-gradient(to right, #000000 0%, #404040, #000000 100%); /*(to right, #016243 0%, #b5deca, #016243 100%);*/
 
−
  border-image-slice: 1;
 
−
  -webkit-touch-callout: none;
 
−
  -webkit-user-select: none;
 
−
  -khtml-user-select: none;
 
−
  -moz-user-select: none;
 
−
  -ms-user-select: none;
 
−
  user-select: none;
 
−
  cursor: default;
 
−
}
 
−
 
−
.header_Notebook_lab_safety {
 
−
  font-size: 45pt;
 
−
  color: black;
 
−
  text-align: center;
 
−
position:bottom;
 
−
  background-image: url("pictures_17/backgrounds/T--Chalmers-Gothenburg--Safety_form_Fume_hood.jpg"); /*, linear-gradient(to bottom, #ffffff, #e6e6e6); /*#0c749c, #145071*/
 
−
/*  min-height: 500px;*/
 
−
 
−
  background-attachment: fixed;
 
−
  background-position: center;
 
−
  background-repeat: no-repeat;
 
−
  background-size: cover;
 
−
background-color:#000000;
 
−
 
−
border-bottom: 3px solid transparent; /*A lot of code for the line between the header and the body */
 
−
  -moz-border-image: -moz-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%);*/
 
−
  -webkit-border-image: -webkit-linear-gradient(left, #000000 0%, #404040, #000000 100%); /*(left, #016243 0%, #b5deca, #016243 100%)*/
 
−
  border-image: linear-gradient(to right, #000000 0%, #404040, #000000 100%); /*(to right, #016243 0%, #b5deca, #016243 100%);*/
 
−
  border-image-slice: 1;
 
−
  -webkit-touch-callout: none;
 
−
  -webkit-user-select: none;
 
−
  -khtml-user-select: none;
 
−
  -moz-user-select: none;
 
−
  -ms-user-select: none;
 
−
  user-select: none;
 
−
  cursor: default;
 
−
}
 
  
 
.header-text {
 
.header-text {
Line 1,142: Line 881:
 
}
 
}
  
 +
.figure-caption {
 +
    display: table-caption;
 +
    caption-side: bottom; 
 +
}
 +
 +
.table-caption {
 +
    display: table-caption;
 +
    caption-side: top;   
 +
  color: black;
 +
  font-size: 11pt;
 +
  text-align: justify;
 +
  padding-left: 0px;
 +
  padding-right:0px;
 +
margin-left:0px;
 +
margin-right:0px;
 +
}
 +
 +
th {
 +
    text-align: center;
 +
}
 +
 +
figure {
 +
  display:table;
 +
  text-align:center;
 +
  margin: auto;
 +
  margin-bottom: 25px;
 +
  margin-top: 25px;
 +
}
 +
 +
/*---Reference List---*/
 +
 +
.reference_list {
 +
    text-align: left;
 +
    font-size:10pt; !important
 +
}
 +
 +
.reference_list ul {
 +
    list-style-type: none;
 +
    list-style-image: none;
 +
}
 +
 +
.reference_list li {
 +
    padding-bottom: 10px;
 +
}
  
 
/*--------------Links in text and lists are red when hovered------------------*/
 
/*--------------Links in text and lists are red when hovered------------------*/
Line 1,158: Line 941:
  
 
p a:visited {
 
p a:visited {
 +
  text-decoration: none;
 +
  color: #c91a03;
 +
}
 +
 +
 +
 +
td a:link {
 
   text-decoration: none;
 
   text-decoration: none;
 
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               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/InterLab">Interlab study</a>
 
               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/InterLab">Interlab study</a>
 
               <hr>
 
               <hr>
−
               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/Achievements/medal">Medals</a>
+
               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/Achievements/medal">Accomplishments</a>
 
             </div>
 
             </div>
 
           </li>
 
           </li>
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−
         <div class="header_Team_collaborations">
+
         <div class="header_test">
−
    <div class="header-text">Achievements</div>
+
  <!-- Banner -->
−
    <div class="header-subtitle">Biobricks</div>
+
    <div id="banner">
−
    </div>
+
            <h3>Biobricks</h3>
 +
            </div>
 +
        </div>
 
        
 
        
 
       <div class="wrap-content">
 
       <div class="wrap-content">
−
        <div class="target" id="thanks">
+
<h4 class="subtitle">Parts summary</h4>
−
          <h4 class="subtitle">Introduction</h4>
+
 
 +
        <h2>
 +
            Submitted parts
 +
        </h2>
 
             <p class="text">
 
             <p class="text">
−
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+
Table 1 shows the parts that were submitted. All biobrick parts work in <i>S. cerevisiae</i> and are compatible with the RFC[10] standard.
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           </p>
 
           </p>
−
        </div>
+
 
−
       
+
          <figure>
−
      <div class="target" id="supervisors">
+
            <figcaption class="table-caption"><b>Table 1.</b> The Basic Parts which were submitted.</figcaption>
−
        <h4 class="sidetitle">Biobricks</h4>
+
            <table>
−
       
+
                <tr>
 +
                  <th><b>Name</b></th> <th><b>Type</b></th>  <th><b>Description</b></th> <th><b>Designer</b></th> <th><b>Length</b></th>
 +
                </tr>
 +
                <tr>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K2329000" target="blank">BBa_K2329000</a></td> <td>Regulation</td> <td>Cre-lox recombination with lox66 and lox71</td> <td>Alex Hedin</td> <td>473 bp</td>
 +
                </tr>
 +
                <tr>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K2329001" target="blank">BBa_K2329001</a></td> <td>RNA</td> <td>gRNA, deletion in <i>STE2</i></td> <td>Alex Hedin</td> <td>388 bp</td>
 +
                </tr>
 +
                <tr>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K2329002" target="blank">BBa_K2329002</a></td> <td>RNA</td> <td>gRNA, deletion in <i>STE3</i></td> <td>Alex Hedin</td> <td>388 bp</td>
 +
                </tr>
 +
            </table>
 +
          </figure>
 +
               
 
         <h2>
 
         <h2>
−
          Biobrick 1
+
            Improvement of a previous biobrick part
 
         </h2>
 
         </h2>
 
         <p class="text">
 
         <p class="text">
−
         
+
<a href="http://parts.igem.org/Part:BBa_K2329000" target="blank">BBa_K2329000</a> is an improvement of the earlier biobrick part, <a href="http://parts.igem.org/Part:BBa_K740000" target="blank">BBa_K740000</a>. The improvement of the cre-lox recombinase system was done by changing the loxP site from reversible switch to a non-reversible switch. The loxP sites flanking the promoter was changed to mutated loxP sites, see Figure 1 [1]. The new mutant loxP sites (lox66 and lox71) have showed great success since the direction of the promoter did not flip back.
−
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<a href="">Here's the brick</a>
+
 
         </p>
 
         </p>
  
 +
<figure>
 +
<img src="https://static.igem.org/mediawiki/2017/e/e1/T--Chalmers-Gothenburg--LoxP_biobricks.png" style="height:50%; width:50%;">
 +
<figcaption><b>Figure 1.</b>  Illustrated figure of the non-reversible flip. When the double mutant loxP site is produced through recombination, the promoter will not be able to flip back.</figcaption>
 +
</figure>
  
 
         <h2>
 
         <h2>
−
           Biobrick 2
+
           Central parts which worked as expected
 
         </h2>
 
         </h2>
 
         <p class="text">
 
         <p class="text">
−
         
+
<a href="http://parts.igem.org/Part:BBa_K2329001"  target="blank">BBa_K2329001</a> and <a href="http://parts.igem.org/Part:BBa_K2329002" target="blank">BBa_K2329002</a> are two central parts of our project, as replacing the native GPCRs STE2 and STE3 is vital to ensure that the only GPCR present on the yeast cell is the heterologous GPCR introduced by us.
−
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<a href="">Here's the brick</a>
+
 
         </p>
 
         </p>
 
 
 
 
 +
        <h2>
 +
          Non submitted parts
 +
        </h2>
 +
        <p class="text">
 +
Table 2 shows the Basic Parts which were not submitted due to time constraints. We chose to present them here anyway to perhaps help studies for iGEM teams in the future.
 +
        </p>
 +
 +
          <figure>
 +
            <figcaption class="table-caption"><b>Table 2.</b> The Basic Parts which were not submitted.</figcaption>
 +
            <table>
 +
                <tr>
 +
                  <th><b>Name</b></th> <th><b>Type</b></th>  <th><b>Description</b></th> <th><b>Designer</b></th> <th><b>Length</b></th>
 +
                </tr>
 +
                <tr>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K2329003" target="blank">BBa_K2329003</a></td> <td>Coding</td> <td>GPCR, Ri7</td> <td>Alex Hedin</td> <td>984 bp</td>
 +
                </tr>
 +
                <tr>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K2329004" target="blank">BBa_K2329004</a></td> <td>Coding</td> <td>GPCR, Olfr1258</td> <td>Alex Hedin</td> <td>936 bp</td>
 +
                </tr>
 +
            </table>
 +
          </figure>
  
 
         <h2>
 
         <h2>
−
           Biobrick 3
+
           Characterization of BBa_K2329004
 
         </h2>
 
         </h2>
 
         <p class="text">
 
         <p class="text">
−
         
+
<a href="http://parts.igem.org/Part:BBa_K2329004" target="blank">BBa_K2329004</a> was another part that got characterized. We incorporated a mouse GPCR called Olfr1258 into <i>S. cerevisiae</i> which showed successful results in membrane localization and response to its ligand 2-butanone.
−
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<a href="">Here's the brick</a>
+
 
         </p>
 
         </p>
−
+
 
−
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        <h4 class="sidetitle">References</h4>
−
      </div>
+
 
 +
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 +
              <ul>
 +
                <li>[1]&#160;&#160; Spehr M, Munger S. Olfactory receptors: G protein-coupled receptors and beyond. Journal of Neurochemistry [Internet]. 2009 [cited 10 October 2017];109(6):1570-1583. Available from: https://www.ncbi.nlm.nih.gov/pubmed/19383089
 +
</li>
 +
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 +
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Latest revision as of 17:28, 30 October 2017

Chalmers Gothenburg iGEM 2017

Parts summary

Submitted parts

Table 1 shows the parts that were submitted. All biobrick parts work in S. cerevisiae and are compatible with the RFC[10] standard.

Table 1. The Basic Parts which were submitted.
Name Type Description Designer Length
BBa_K2329000 Regulation Cre-lox recombination with lox66 and lox71 Alex Hedin 473 bp
BBa_K2329001 RNA gRNA, deletion in STE2 Alex Hedin 388 bp
BBa_K2329002 RNA gRNA, deletion in STE3 Alex Hedin 388 bp

Improvement of a previous biobrick part

BBa_K2329000 is an improvement of the earlier biobrick part, BBa_K740000. The improvement of the cre-lox recombinase system was done by changing the loxP site from reversible switch to a non-reversible switch. The loxP sites flanking the promoter was changed to mutated loxP sites, see Figure 1 [1]. The new mutant loxP sites (lox66 and lox71) have showed great success since the direction of the promoter did not flip back.

Figure 1. Illustrated figure of the non-reversible flip. When the double mutant loxP site is produced through recombination, the promoter will not be able to flip back.

Central parts which worked as expected

BBa_K2329001 and BBa_K2329002 are two central parts of our project, as replacing the native GPCRs STE2 and STE3 is vital to ensure that the only GPCR present on the yeast cell is the heterologous GPCR introduced by us.

Non submitted parts

Table 2 shows the Basic Parts which were not submitted due to time constraints. We chose to present them here anyway to perhaps help studies for iGEM teams in the future.

Table 2. The Basic Parts which were not submitted.
Name Type Description Designer Length
BBa_K2329003 Coding GPCR, Ri7 Alex Hedin 984 bp
BBa_K2329004 Coding GPCR, Olfr1258 Alex Hedin 936 bp

Characterization of BBa_K2329004

BBa_K2329004 was another part that got characterized. We incorporated a mouse GPCR called Olfr1258 into S. cerevisiae which showed successful results in membrane localization and response to its ligand 2-butanone.

References

  • [1]   Spehr M, Munger S. Olfactory receptors: G protein-coupled receptors and beyond. Journal of Neurochemistry [Internet]. 2009 [cited 10 October 2017];109(6):1570-1583. Available from: https://www.ncbi.nlm.nih.gov/pubmed/19383089