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| − | <h1>Brainstorming</h1> | + | <h1>Attributions</h1> |
| − | <h3>Here are some other ideas we came up with this summer that future iGEM teams can incorporate into their own projects:</h3> | + | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sint, explicabo dolores ipsam aliquam inventore corrupti.</p> |
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| + | <h1></h1> |
| − | <div class="container"> | + | <p> |
| − | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | + | The Columbia 2017 iGEM team consists of a group of biochemists, biologists and bioengineers. We'd like to thank all those |
| − | <div class="panel panel-default">
| + | who have helped us, not only for making iGEM possible but also for making it incredibly fun. |
| − | <div class="panel-heading" role="tab" id="headingOne">
| + | Benjie, Jenny, Noah, Brandon, Tarun: wet lab |
| − | <h3 class="panel-title">
| + | Alex, Nathan, Panos: dry lab |
| − | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="true" aria-controls="collapseOne">
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| − | No Pain, but Sure Gain: T-cell Targeted Nociceptive Sodium Channels
| + | We came up with our project idea after many brainstorming meetings including |
| − | </a>
| + | all members the team equally. |
| − | </h3>
| + | Tissue Culture: Tarun, Benjie |
| − | </div>
| + | Biobricks: Jenny, Noah, Benjie |
| − | <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
| + | Modeling: Alex |
| − | <div class="panel-body">
| + | Human Practices: Brandon, Noah |
| − | Nav1.7 is a voltage-gated sodium channel expressed on nociceptive neurons, neurons responsible for transmitting pain signals
| + | Wiki: Brandon, Alex |
| − | from the PNS to the CNS. Nav1.7 is essential for normal pain sensation; if it is not expressed, the patient will
| + | Presentation: Noah, Jenny, Benjie |
| − | not feel pain at all, and if it is overexpressed, the patient will experience chronic pain (20% of the population
| + | |
| − | worldwide). Nav1.7 is therefore a prime therapeutic target whose blockage by an T-cell antibody would mitigate
| + | Supervision (experiment researcha and design): Harris Wang, Ross McBee, Carlotta Ronda, Sonja Billerbeck |
| − | pain, with the T cell acting as a potential analgesic. A circuit would be designed so that once the T cell binds
| + | |
| − | to the epitope, a chimeric antigen receptor (CAR) could be engineered to be drug-switchable so that the T cells
| + | Acknowledgements: |
| − | are controllable. This way, a physician could titrate cell activity and control timing with a drug, which is
| + | Wang Lab for advice on lab technique, best practice, and general suport. Virginia Cornish, Tal Danino Lars Dietrich, |
| − | potentially safer. An accessible drug that suppresses pain would not only be of much relevance to medicine, but
| + | Ken Shepard, Danino Lab for their great mentorship and support. Thanks to Columbia University and iGEM for their sponsoship. |
| − | also in our daily lives.
| + | </p> |
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| − | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
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| − | Cancer Diagnostics Using GPCR in Yeast
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| − | </h3>
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| − | Cancer is the second greatest cause of death in the United States. Nevertheless, many biomarkers have been found for various
| + | |
| − | types of cancer, such as pancreatic, breast and prostate cancer. These biomarkers that are intrinsic to a particular
| + | |
| − | form of cancer can be applied to cancer diagnostics and detection, given a specific receptor and reporter. G
| + | |
| − | protein coupled receptors, along with yeast, can be used as a reliable method of cancer detection when enhanced
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| − | with directed evolution (to induce sensitivity to high concentrations of biomarker, but not low concentrations)
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| − | and expressed alongside GFP in the presence of a strong signal.
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| − | A Long-Awaited Remedy for Hemophilia: Probiotic Hemophilia Treatment
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| − | </h3>
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| − | Hemophilia A, the most common type of hemophilia, is caused by a missing or defective factor VIII clotting protein. Current
| + | |
| − | treatments are time-consuming, expensive, and unpleasant. Our idea is to create a probiotic that secretes coagulation
| + | |
| − | factor VIII into the small intestine to be absorbed into the bloodstream. We would obtain cells with mutations
| + | |
| − | in the thioredoxin reductase gene (trxB) and glutathione reductase gene (gor) in order to make E.coli’s intracellular
| + | |
| − | environment oxidizing. This is necessary for the oxidation of the disulfide bonds in the Factor VIII proteins,
| + | |
| − | which along with the expression of a heterologous protein disulfide isomerase and a heterologous chaperone protein,
| + | |
| − | enhances the yield and solubility of Factor VIII proteins. The proteins will be expressed in
| + | |
| − | <em>E.coli</em>, given the appropriate auxotrophic control sequences and gene casette. The production of the factor
| + | |
| − | VIII proteins will be tested via Western Blot, and the activity of the proteins will be observed with a APTT-based
| + | |
| − | one-stage assay. Finally, the solubility of the proteins was evaluated by verifying the presence of the proteins
| + | |
| − | in an aqueous fraction (after centrifugation).
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| − | Ecoligen: Collagen-like protein production by engineered bacteria
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| − | </a>
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| − | </h3>
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| − | </div>
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| − | Most collagen products used for biomaterials or biomedical devices are extracted from animal sources. However, application
| + | |
| − | of animal collagen carries the risk of pathogen or prion contamination and the possibility of causing allergies.
| + | |
| − | Other problems include the lack of standardization for animal collagen extraction processes and the inability
| + | |
| − | to modify collagen sequences to achieve different biological purposes. Compared with collagens extracted from
| + | |
| − | animal tissues, recombinant collagens are highly pure, disease free, consistent among batches, and amendable
| + | |
| − | to sequence modifications and large scale production. Such recombinant collagen can be made by
| + | |
| − | <em>
| + | |
| − | E.coli</em>bacteria, allowing for the use of versatile and cheap collagen, especially in the fields of regenerative
| + | |
| − | medicine and tissue engineering.
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div> | + | |
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| | </div> | | </div> |
| | | | |
The Columbia 2017 iGEM team consists of a group of biochemists, biologists and bioengineers. We'd like to thank all those
who have helped us, not only for making iGEM possible but also for making it incredibly fun.
Benjie, Jenny, Noah, Brandon, Tarun: wet lab
Alex, Nathan, Panos: dry lab
We came up with our project idea after many brainstorming meetings including
all members the team equally.
Tissue Culture: Tarun, Benjie
Biobricks: Jenny, Noah, Benjie
Modeling: Alex
Human Practices: Brandon, Noah
Wiki: Brandon, Alex
Presentation: Noah, Jenny, Benjie
Supervision (experiment researcha and design): Harris Wang, Ross McBee, Carlotta Ronda, Sonja Billerbeck
Acknowledgements:
Wang Lab for advice on lab technique, best practice, and general suport. Virginia Cornish, Tal Danino Lars Dietrich,
Ken Shepard, Danino Lab for their great mentorship and support. Thanks to Columbia University and iGEM for their sponsoship.
