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− | <h1>Brainstorming</h1> | + | <h1>Attributions</h1> |
− | <h3>Here are some other ideas we came up with this summer that future iGEM teams can incorporate into their own projects:</h3> | + | <p></p> |
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| + | <h1>Team Acknowledgements</h1> |
| + | <br> |
| + | <p>We would like to acknowledge the work done by our team. </p> |
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− | <div class="container"> | + | <h3>Entrepreneurship</h3> |
− | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
| + | <p>Benjamin, Brandon</p> |
− | <div class="panel panel-default"> | + | |
− | <div class="panel-heading" role="tab" id="headingOne">
| + | <h3>Biobricks</h3> |
− | <h3 class="panel-title">
| + | <p>Jennifer</p> |
− | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="true" aria-controls="collapseOne">
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− | No Pain, but Sure Gain: T-cell Targeted Nociceptive Sodium Channels
| + | <h3>Modeling</h3> |
− | </a>
| + | <p>Alex</p> |
− | </h3>
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− | </div>
| + | <h3>Integrated Human Practices</h3> |
− | <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
| + | <p>Noah, Brandon</p> |
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− | Nav1.7 is a voltage-gated sodium channel expressed on nociceptive neurons, neurons responsible for transmitting pain signals
| + | <h3>Public Engagement</h3> |
− | from the PNS to the CNS. Nav1.7 is essential for normal pain sensation; if it is not expressed, the patient will
| + | <p>Noah, Jennifer, Benjamin, Brandon, Tarun, Nathan, Alex</p> |
− | not feel pain at all, and if it is overexpressed, the patient will experience chronic pain (20% of the population
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− | worldwide). Nav1.7 is therefore a prime therapeutic target whose blockage by an T-cell antibody would mitigate
| + | <h3>Presentation</h3> |
− | pain, with the T cell acting as a potential analgesic. A circuit would be designed so that once the T cell binds
| + | <p>Noah, Jennifer</p> |
− | to the epitope, a chimeric antigen receptor (CAR) could be engineered to be drug-switchable so that the T cells
| + | </div> |
− | are controllable. This way, a physician could titrate cell activity and control timing with a drug, which is
| + | <div class="col-lg-6"> |
− | potentially safer. An accessible drug that suppresses pain would not only be of much relevance to medicine, but
| + | <h1>Lab Acknowledgements</h1> |
− | also in our daily lives.
| + | <br> |
− | </div>
| + | <p>A big thank you to Harris Wang, Virginia Cornish, Tal Danino, Lars Dietrich, Ken Shepard, Sonja Billerbeck, Ross McBee, |
− | </div>
| + | Carlotta Ronda. We thank the Wang Lab, Danino Lab, and Prince Lab for the lab spaces and mentorship that they provided |
− | </div> | + | throughout the summer. In conclusion, we thank Columbia University and iGEM for their sponsorship.</p> |
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− | Cancer Diagnostics Using GPCR in Yeast
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− | </a>
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− | Cancer is the second greatest cause of death in the United States. Nevertheless, many biomarkers have been found for various
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− | types of cancer, such as pancreatic, breast and prostate cancer. These biomarkers that are intrinsic to a particular
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− | form of cancer can be applied to cancer diagnostics and detection, given a specific receptor and reporter. G
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− | protein coupled receptors, along with yeast, can be used as a reliable method of cancer detection when enhanced
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− | with directed evolution (to induce sensitivity to high concentrations of biomarker, but not low concentrations)
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− | and expressed alongside GFP in the presence of a strong signal.
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− | A Long-Awaited Remedy for Hemophilia: Probiotic Hemophilia Treatment
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− | Hemophilia A, the most common type of hemophilia, is caused by a missing or defective factor VIII clotting protein. Current
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− | treatments are time-consuming, expensive, and unpleasant. Our idea is to create a probiotic that secretes coagulation
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− | factor VIII into the small intestine to be absorbed into the bloodstream. We would obtain cells with mutations
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− | in the thioredoxin reductase gene (trxB) and glutathione reductase gene (gor) in order to make E.coli’s intracellular
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− | environment oxidizing. This is necessary for the oxidation of the disulfide bonds in the Factor VIII proteins,
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− | which along with the expression of a heterologous protein disulfide isomerase and a heterologous chaperone protein,
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− | enhances the yield and solubility of Factor VIII proteins. The proteins will be expressed in
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− | <em>E.coli</em>, given the appropriate auxotrophic control sequences and gene casette. The production of the factor
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− | VIII proteins will be tested via Western Blot, and the activity of the proteins will be observed with a APTT-based
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− | one-stage assay. Finally, the solubility of the proteins was evaluated by verifying the presence of the proteins
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− | in an aqueous fraction (after centrifugation).
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− | Ecoligen: Collagen-like protein production by engineered bacteria
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− | Most collagen products used for biomaterials or biomedical devices are extracted from animal sources. However, application
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− | of animal collagen carries the risk of pathogen or prion contamination and the possibility of causing allergies.
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− | Other problems include the lack of standardization for animal collagen extraction processes and the inability
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− | to modify collagen sequences to achieve different biological purposes. Compared with collagens extracted from
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− | animal tissues, recombinant collagens are highly pure, disease free, consistent among batches, and amendable
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− | to sequence modifications and large scale production. Such recombinant collagen can be made by
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− | <em>
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− | E.coli</em>bacteria, allowing for the use of versatile and cheap collagen, especially in the fields of regenerative
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− | medicine and tissue engineering.
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