Difference between revisions of "Team:Dalhousie/test3"

Line 13: Line 13:
  
 
}
 
}
 +
 +
 
</style>
 
</style>
  
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#sideMenu, #top_title, .patrollink  {display:none;}
 
#sideMenu, #top_title, .patrollink  {display:none;}
 
#content { width:100%; padding:0px;  margin-top:-20px; margin-left:0px; background-color: white;}
 
#content { width:100%; padding:0px;  margin-top:-20px; margin-left:0px; background-color: white;}
body {background-color:white; }
+
body {background-color:white; height:100%; }
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 10px;  background-color: transparent; z-index:100;}
+
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px;  background-color: transparent; z-index:100;}
 
         .jumbotron {
 
         .jumbotron {
 
             height: 850px;
 
             height: 850px;
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             }
 
             }
</style>
+
.navbar-default .navbar-nav>.active>a, .nav > li > a:hover, .nav > li > a:focus {
 
+
  background-color: #C1D35D;
<!------------fadedivs-------------------------->
+
  text-decoration: none;
<style>
+
#grad1 {
+
    height: 833px;
+
    background: transparent; /* For browsers that do not support gradients */
+
    background: -webkit-linear-gradient(transparent 90%, rgba(27,22,16,1) ); /* For Safari 5.1 to 6.0 */
+
    background: -o-linear-gradient(transparent 90%, rgba(27,22,16,1)); /* For Opera 11.1 to 12.0 */
+
    background: -moz-linear-gradient(transparent 90%, rgba(27,22,16,1) ); /* For Firefox 3.6 to 15 */
+
    background: linear-gradient(transparent 90%, rgba(27,22,16,1)); /* Standard syntax (must be last) */
+
 
}
 
}
</style>
 
<style>
 
#grad2 {
 
position: absolute;
 
z-index:1;
 
height: 100%;
 
width:100%;
 
    background: transparent; /* For browsers that do not support gradients */
 
    background: -webkit-linear-gradient(rgba(27,22,16,1), transparent 80%, rgba(27,22,16,1)); /* For Safari 5.1 to 6.0 */
 
    background: -o-linear-gradient(rgba(27,22,16,1), transparent 80%, rgba(27,22,16,1)); /* For Opera 11.1 to 12.0 */
 
    background: -moz-linear-gradient(rgba(27,22,16,1), transparent 80%, rgba(27,22,16,1)); /* For Firefox 3.6 to 15 */
 
    background: linear-gradient(rgba(27,22,16,1), transparent 80%, rgba(27,22,16,1)); /* Standard syntax (must be last) */
 
}
 
 
 
 
</style>
 
</style>
  
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<body>
 
<body>
 +
  
 
<!-------------------navbar---------------------->
 
<!-------------------navbar---------------------->
Line 84: Line 64:
 
         </a>
 
         </a>
 
         <ul class="dropdown-menu" style=" background-color: rgba(193,211,93,0.8);">
 
         <ul class="dropdown-menu" style=" background-color: rgba(193,211,93,0.8);">
           <li><a href="https://2017.igem.org/Team:Dalhousie/Description" style=" color: white;" >Description</a></li>
+
           <li><a href="https://2017.igem.org/Team:Dalhousie/Description" >Description</a></li>
           <li><a href="https://2017.igem.org/Team:Dalhousie/Design"style=" color: white;">Design</a></li>
+
           <li><a href="https://2017.igem.org/Team:Dalhousie/Design">Design</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Improve">Improve</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Improve">Improve</a></li>
 
         </ul>
 
         </ul>
Line 93: Line 73:
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Results
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Results
 
         </a>
 
         </a>
         <ul class="dropdown-menu">
+
         <ul class="dropdown-menu" style=" background-color: rgba(193,211,93,0.8);">
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/InterLab">Interlab</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/InterLab">Interlab</a></li>
 
   <li><a href="https://2017.igem.org/Team:Dalhousie/Results">Results</a></li>
 
   <li><a href="https://2017.igem.org/Team:Dalhousie/Results">Results</a></li>
Line 103: Line 83:
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Team
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Team
 
         </a>
 
         </a>
         <ul class="dropdown-menu">
+
         <ul class="dropdown-menu" style=" background-color: rgba(193,211,93,0.8);">
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Team">Team</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Team">Team</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Collaborations">Collaborations</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Collaborations">Collaborations</a></li>
Line 114: Line 94:
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Human Practices
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Human Practices
 
         </a>
 
         </a>
         <ul class="dropdown-menu">
+
         <ul class="dropdown-menu" style=" background-color: rgba(193,211,93,0.8);">
 
         <li><a href="https://2017.igem.org/Team:Dalhousie/Human_Practices">Summary</a></li>
 
         <li><a href="https://2017.igem.org/Team:Dalhousie/Human_Practices">Summary</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Silver_Integrated">Silver HP</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Silver_Integrated">Silver HP</a></li>
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         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Lab
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Lab
 
         </a>
 
         </a>
         <ul class="dropdown-menu">
+
         <ul class="dropdown-menu" style=" background-color: rgba(193,211,93,0.8);">
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Parts">Parts</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Parts">Parts</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Basic_Parts">Basic Parts</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/Basic_Parts">Basic Parts</a></li>
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         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Awards
 
         <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Awards
 
         </a>
 
         </a>
         <ul class="dropdown-menu">
+
         <ul class="dropdown-menu" style=" background-color: rgba(193,211,93,0.8);">
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Applied_Design">Applied Design</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Applied_Design">Applied Design</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Entrepreneurship">Entrepreneurship</a></li>
 
           <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Entrepreneurship">Entrepreneurship</a></li>
Line 165: Line 145:
 
     </div>
 
     </div>
 
<li>
 
<li>
                         <a href="mailto:igemdalhousie@gmail.com" ><i class="fa fa-envelope-o fa-fw" style=" color: rgba(149, 48, 41, 1);"></i> <span class="network-name"></span></a>
+
                         <a href="mailto:igemdalhousie@gmail.com" ><i class="fa fa-envelope-o fa-fw" style=" color: white;" ></i> <span class="network-name"></span></a>
 
                     </li>
 
                     </li>
 
<li>
 
<li>
                         <a target="_blank" href="https://twitter.com/dalhousie_igem?lang=en" ><i class="fa fa-twitter fa-fw" style=" color: rgba(61, 145, 229, 1);"></i> <span class="network-name"></span></a>
+
                         <a target="_blank" href="https://twitter.com/dalhousie_igem?lang=en" ><i class="fa fa-twitter fa-fw" style=" color: white;" ></i> <span class="network-name"></span></a>
 
                     </li>
 
                     </li>
 
                     <li>
 
                     <li>
                         <a target="_blank" href="https://www.facebook.com/Dalhousie.iGEM/" ><i class="fa fa-facebook fa-fw" style=" color: rgba(57, 86, 156, 1);"></i> <span class="network-name"></span></a>
+
                         <a target="_blank" href="https://www.facebook.com/Dalhousie.iGEM/" ><i class="fa fa-facebook fa-fw" style=" color: white;" ></i> <span class="network-name"></span></a>
 
                     </li>
 
                     </li>
  
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<div id="grad1"><!--------fade------------>
 
 
         <div class="panel" id="panel1" >
 
         <div class="panel" id="panel1" >
  
 
             <div class="inner" >
 
             <div class="inner" >
<div class="top" ><div class="title" >Welcome to Dalhousie's 2017 iGEM wiki</div></div>
+
<div class="top" ><div class="title" >Dalhousie iGEM 2017</div></div>
  
 
    
 
    
  
 
<video  id= "myVideo" class="bg-video" style="top:0px; left:0px; padding-bottom:0px; position:fixed; width:100%;z-index:-100; " autoplay="true" loop="loop" preload="metadata" muted="muted">
 
<video  id= "myVideo" class="bg-video" style="top:0px; left:0px; padding-bottom:0px; position:fixed; width:100%;z-index:-100; " autoplay="true" loop="loop" preload="metadata" muted="muted">
         <source src="https://static.igem.org/mediawiki/2017/5/53/Final5.mp4" type="video/mp4" />
+
         <source src="https://static.igem.org/mediawiki/2017/6/68/Thisfinal.mp4" type="video/mp4" />
  
  
  
 
<!--------------------------------fade title---------------------------->
 
<!--------------------------------fade title---------------------------->
<style>body {
+
<style>
 +
 
 +
body {
 
   margin: 0;
 
   margin: 0;
 
   height: 10px;
 
   height: 10px;
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.top {
 
.top {
  
   margin-top: 450px;
+
   margin-top: 350px;
 
   position: absolute;
 
   position: absolute;
  width:55%;
+
  width:65%;
   left:300px;
+
   left:250px;
   background-color: rgba(27,22,16,0.4);
+
   background-color: transparent;
 
   height: 150px;
 
   height: 150px;
 
   text-align: center;
 
   text-align: center;
 
   font-family: 'Trebuchet MS';
 
   font-family: 'Trebuchet MS';
   font-size: 50px;
+
   font-size: 80px;
   font-weight: 100;
+
   font-weight: 400;
 
   color: white;
 
   color: white;
 
line-height: 60px;
 
line-height: 60px;
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</style>
 
</style>
 +
  
 
<!---------------------------------------------------------------------------------this------------------------------------------------------------------------------------------->
 
<!---------------------------------------------------------------------------------this------------------------------------------------------------------------------------------->
 
<script> $(window).scroll(function(){
 
<script> $(window).scroll(function(){
     $(".top").css("opacity", 1 - $(window).scrollTop() / 400);
+
     $(".top").css("opacity", 1 - $(window).scrollTop() / 300);
   });</script>
+
   });
 +
</script>
  
  
 
             </div>
 
             </div>
 
         </div>  
 
         </div>  
</div>
+
 
  
  
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         <div class="panel" id="panel2">
 
         <div class="panel" id="panel2">
 
             <div class="inner">
 
             <div class="inner">
 +
 +
  <!-- Vertical Timeline -->
 +
  <section id="conference-timeline">
 +
 
 +
    <div class="conference-center-line"></div>
 +
    <div class="conference-timeline-content">
 +
      <!-- Article -->
 +
      <div class="timeline-article">
 +
        <div class="content-left-container">
 +
          <div class="content-left">
 +
            <p>Softwood waste from the lumber industry contains cellulose, which can be turned into glucose and used to make biofuel.  <span class="article-number">01</span></p>
 +
          </div>
 +
       
 +
        </div>
 +
  <div class="content-right-container">
 
            
 
            
 +
       
 +
        </div>
 +
        <div class="meta-date">
 +
          <span class="date"></span>
 +
          <span class="month"></span>
 +
        </div>
 +
      </div>
 +
      <!-- // Article -->
 +
     
 +
      <!-- Article -->
 +
      <div class="timeline-article">
 +
        <div class="content-left-container">
 +
         
 +
       
 +
        </div>
 +
        <div class="content-right-container">
 +
          <div class="content-right">
 +
            <p>We hypothesized that the porcupine microbiome must contain an enzyme that can break down cellulose, as bark is food. <span class="article-number">02</span></p>
 +
          </div>
 +
       
 +
        </div>
 +
        <div class="meta-date">
 +
          <span class="date"></span>
 +
          <span class="month"></span>
 +
        </div>
 +
      </div>
 +
      <!-- // Article -->
 +
     
 +
      <!-- Article -->
 +
      <div class="timeline-article">
 +
        <div class="content-left-container">
 +
          <div class="content-left">
 +
            <p>We mined the porcupine’s microbiome to identify the enzyme that was able to break down wood.  <span class="article-number">03</span></p>
 +
          </div>
 +
   
 +
        </div>
 +
        <div class="content-right-container">
 +
         
 +
 +
        </div>
 +
        <div class="meta-date">
 +
          <span class="date"></span>
 +
          <span class="month"></span>
 +
        </div>
 +
      </div>
 +
      <!-- // Article -->
 +
<!-- Article -->
 +
      <div class="timeline-article">
 +
        <div class="content-left-container">
 +
         
 +
       
 +
        </div>
 +
        <div class="content-right-container">
 +
        <div class="content-right">
 +
            <p>We created a metagenomic library of the genes able to code this enzyme as well as novel enzymes. <span class="article-number">04</span></p>
 +
          </div>
 +
       
 +
        </div>
 +
        <div class="meta-date">
 +
          <span class="date"></span>
 +
          <span class="month"></span>
 +
        </div>
 +
      </div>
 +
      <!-- // Article -->
 +
<!-- Article -->
 +
      <div class="timeline-article">
 +
        <div class="content-left-container">
 +
        <div class="content-left">
 +
            <p>We put these genes into E.coli to see if they could then digest cellulose down. <span class="article-number">05</span></p>
 +
          </div>
 +
   
 +
        </div>
 +
        <div class="content-right-container">
 +
         
 +
 +
        </div>
 +
        <div class="meta-date">
 +
          <span class="date"></span>
 +
          <span class="month"></span>
 +
        </div>
 +
      </div>
 +
      <!-- // Article -->
 +
<!-- Article -->
 +
      <div class="timeline-article">
 +
        <div class="content-left-container">
 +
         
 +
       
 +
        </div>
 +
        <div class="content-right-container">
 +
        <div class="content-right">
 +
            <p>We put the E.coli into a bioreactor with cellulose to see if it gets broken down. In the future this can be combined with yeast to transform in finally into biofuel! <span class="article-number">06</span></p>
 +
          </div>
 +
       
 +
        </div>
 +
        <div class="meta-date">
 +
          <span class="date"></span>
 +
          <span class="month"></span>
 +
        </div>
 +
      </div>
 +
      <!-- // Article -->
 +
    </div>
 +
 
 +
  </section>
 +
  <!-- // Vertical Timeline -->
  
<!-------------------------------------------infographic----------------------------->
+
  <style>
<p style="text-align:center; color:white; margin-top:50px;">
+
body {
<img src= "https://static.igem.org/mediawiki/2017/8/8b/Infograph2_1.jpg" align="center" height="70%" width="70%"></p>
+
  background: #e6e6e6;
 +
  font-family: "Roboto", sans-serif;
 +
  font-weight: 400;
 +
}
  
 +
/*===== Vertical Timeline =====*/
 +
#conference-timeline {
 +
  position: relative;
 +
  max-width: 920px;
 +
  width: 100%;
 +
  margin: 0 auto;
 +
}
 +
#conference-timeline .timeline-start,
 +
#conference-timeline .timeline-end {
 +
  display: table;
 +
  font-family: "Roboto", sans-serif;
 +
  font-size: 18px;
 +
  font-weight: 900;
 +
  text-transform: uppercase;
 +
  background: #232F13;
 +
  padding: 15px 23px;
 +
  color: #fff;
 +
  max-width: 5%;
 +
  width: 100%;
 +
  text-align: center;
 +
  margin: 0 auto;
 +
}
 +
#conference-timeline .conference-center-line {
 +
  position: absolute;
 +
  width: 3px;
 +
  height: 100%;
 +
  top: 0;
 +
  left: 50%;
 +
  margin-left: -2px;
 +
  background: #232F13;
 +
  z-index: -1;
 +
}
 +
#conference-timeline .conference-timeline-content {
 +
  padding-top: 67px;
 +
  padding-bottom: 67px;
 +
}
 +
.timeline-article {
 +
  width: 100%;
 +
  height: 100%;
 +
  position: relative;
 +
  overflow: hidden;
 +
  margin: 20px 0;
 +
}
 +
.timeline-article .content-left-container,
 +
.timeline-article .content-right-container {
 +
  max-width: 44%;
 +
  width: 100%;
 +
}
 +
.timeline-article .timeline-author {
 +
  display: block;
 +
  font-weight: 400;
 +
  font-size: 14px;
 +
  line-height: 24px;
 +
  color: #232F13;
 +
  text-align: right;
 +
}
 +
.timeline-article .content-left,
 +
.timeline-article .content-right {
 +
  position: relative;
 +
  width: auto;
 +
  border: 1px solid #ddd;
 +
  background-color: #fff;
 +
  box-shadow: 0 1px 3px rgba(0,0,0,.03);
 +
  padding: 27px 25px;
 +
}
 +
.timeline-article p {
 +
  margin: 0 0 0 60px;
 +
  padding: 0;
 +
  font-weight: 400;
 +
  color: #232F13;
 +
  font-size: 14px;
 +
  line-height: 24px;
 +
  position: relative;
 +
}
 +
.timeline-article p span.article-number {
 +
  position: absolute;
 +
  font-weight: 300;
 +
  font-size: 44px;
 +
  top: 10px;
 +
  left: -60px;
 +
  color: #232F13;
 +
}
 +
.timeline-article .content-left-container {
 +
  float: left;
 +
}
 +
.timeline-article .content-right-container {
 +
  float: right;
 +
}
 +
.timeline-article .content-left:before,
 +
.timeline-article .content-right:before{
 +
  position: absolute;
 +
  top: 20px;
 +
  font-size: 23px;
 +
  font-family: "FontAwesome";
 +
  color: #fff;
 +
}
 +
.timeline-article .content-left:before {
 +
  content: "\f0da";
 +
  right: -8px;
 +
}
 +
.timeline-article .content-right:before {
 +
  content: "\f0d9";
 +
  left: -8px;
 +
}
 +
.timeline-article .meta-date {
 +
  position: absolute;
 +
  top: 0;
 +
  left: 50%;
 +
  width: 62px;
 +
  height: 62px;
 +
  margin-left: -31px;
 +
  color: #fff;
 +
  border-radius: 100%;
 +
  background:#232F13;
 +
}
 +
.timeline-article .meta-date .date,
 +
.timeline-article .meta-date .month {
 +
  display: block;
 +
  text-align: center;
 +
  font-weight: 900;
 +
}
 +
.timeline-article .meta-date .date {
 +
  font-size: 30px;
 +
  line-height: 40px;
 +
}
 +
.timeline-article .meta-date .month {
 +
  font-size: 18px;
 +
  line-height: 10px;
 +
}
 +
/*===== // Vertical Timeline =====*/
 +
 +
/*===== Resonsive Vertical Timeline =====*/
 +
@media only screen and (max-width: 830px) {
 +
  #conference-timeline .timeline-start,
 +
  #conference-timeline .timeline-end {
 +
    margin: 0;
 +
  }
 +
  #conference-timeline .conference-center-line {
 +
    margin-left: 0;
 +
    left: 50px;
 +
  }
 +
  .timeline-article .meta-date {
 +
    margin-left: 0;
 +
    left: 20px;
 +
  }
 +
  .timeline-article .content-left-container,
 +
  .timeline-article .content-right-container {
 +
    max-width: 100%;
 +
    width: auto;
 +
    float: none;
 +
    margin-left: 110px;
 +
    min-height: 53px;
 +
  }
 +
  .timeline-article .content-left-container {
 +
    margin-bottom: 20px;
 +
  }
 +
  .timeline-article .content-left,
 +
  .timeline-article .content-right {
 +
    padding: 10px 25px;
 +
    min-height: 65px;
 +
  }
 +
  .timeline-article .content-left:before {
 +
    content: "\f0d9";
 +
    right: auto;
 +
    left: -8px;
 +
  }
 +
  .timeline-article .content-right:before {
 +
    display: none;
 +
  }
 +
}
 +
@media only screen and (max-width: 400px) {
 +
  .timeline-article p {
 +
    margin: 0;
 +
  }
 +
  .timeline-article p span.article-number {
 +
    display: none;
 +
  }
 +
 
 +
}
 +
/*===== // Resonsive Vertical Timeline =====*/
 +
</style>
  
           
 
 
             </div>
 
             </div>
 
         </div>
 
         </div>
 +
  
  
 
<div class="container" style="height:450px; width:100%; position: relative;">
 
<div class="container" style="height:450px; width:100%; position: relative;">
<div id="grad2" style=" width:100%; position: absolute; left: 0px; top: 0px;"><!--------fade------------>
+
 
</div>
+
 
  </div>
 
  </div>
  
  
 
         <div class="panel panel-red" id="panel3">
 
         <div class="panel panel-red" id="panel3">
             <div class="inner">
+
              
       <p style="text-align:center; color:white; font-size: 30px; margin-top:100px;">
+
        
Thanks to our Sponsors!!</p>
+
 
<!---------------------------pics---------------------------->
 
<!---------------------------pics---------------------------->
 
<p style="text-align: center; ">
 
<p style="text-align: center; ">
Line 271: Line 553:
 
<img src= "https://static.igem.org/mediawiki/2017/5/57/B.png"  height="20%" width="20%" style=" padding:50px;">
 
<img src= "https://static.igem.org/mediawiki/2017/5/57/B.png"  height="20%" width="20%" style=" padding:50px;">
 
</p>
 
</p>
             </div>
+
              
 
         </div>
 
         </div>
  
Line 315: Line 597:
  
 
.noBackground {
 
.noBackground {
 +
 
background-color: rgba(0,0,0,0);
 
background-color: rgba(0,0,0,0);
 
visibility: visible;
 
visibility: visible;
Line 326: Line 609:
  
 
.blackBackground {
 
.blackBackground {
background-color: rgba(0,0,0,0.7);
+
background-color: rgba(35,47,19,0.7);
 
visibility: visible;
 
visibility: visible;
 
width:100%;
 
width:100%;
Line 335: Line 618:
 
z-index:100;
 
z-index:100;
 
padding-bottom:40px;
 
padding-bottom:40px;
 
+
color: red;
 
}
 
}
  
Line 361: Line 644:
 
height: 100%;
 
height: 100%;
 
margin-bottom:0px;
 
margin-bottom:0px;
background: black;
+
background: white;
 
}
 
}
 
#panel3{
 
#panel3{
 
width:100%;
 
width:100%;
height: 500px;
+
height: 200px;
margin-bottom:-100px;
+
 
background: black;
+
background: rgba(35,47,19,0.9);
 +
bottom:-100px;
 
}
 
}
  

Revision as of 21:53, 9 October 2017

Dalhousie iGEM 2017

Softwood waste from the lumber industry contains cellulose, which can be turned into glucose and used to make biofuel. 01

We hypothesized that the porcupine microbiome must contain an enzyme that can break down cellulose, as bark is food. 02

We mined the porcupine’s microbiome to identify the enzyme that was able to break down wood. 03

We created a metagenomic library of the genes able to code this enzyme as well as novel enzymes. 04

We put these genes into E.coli to see if they could then digest cellulose down. 05

We put the E.coli into a bioreactor with cellulose to see if it gets broken down. In the future this can be combined with yeast to transform in finally into biofuel! 06