Difference between revisions of "Team:Dalhousie/test5"

Revision as of 01:43, 11 October 2017

Dalhousie iGEM 2017

The goal of this project is to construct a bioreactor where cellulose would be the input, D-glucose the output and E.coli expressing a suite of cellulolytic enzymes would be the workhorse, converting one to the other.

Softwood waste from the lumber industry contains cellulose, which can be turned into glucose and used to make biofuel. 01

We hypothesized that the porcupine microbiome must contain an enzyme that can break down cellulose, as bark is food. 02

We mined the porcupine’s microbiome to identify the enzyme that was able to break down wood. 03

We created a metagenomic library of the genes able to code this enzyme as well as novel enzymes. 04

We put these genes into E.coli to see if they could then digest cellulose down. 05

We put the E.coli into a bioreactor with cellulose to see if it gets broken down. In the future this can be combined with yeast to transform in finally into biofuel! 06

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-<p>The goal of this project is to construct a bioreactor where cellulose would be the input, D-glucose the output and E.coli expressing a suite of cellulolytic enzymes would be the workhorse, converting one to the other. </p>
+<p style= "color:black;">The goal of this project is to construct a bioreactor where cellulose would be the input, D-glucose the output and E.coli expressing a suite of cellulolytic enzymes would be the workhorse, converting one to the other. </p>
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