Team:Dartmouth/Protocols
Dartmouth
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Pf0-1 Pseudomonas Fluorescens Electrocompetency Prep
- Innoculate 6 mL of liquid LB or Pseudomonas Minimal Media with Pf0-1 and grow overnight
- Split overnight into 1.5 mL aliquots and centrifuge at 16,000x g, discard supernatant
- Resuspend cell pellet with 1 mL of 300 mM sucrose in water solution
- Centrifuge at 16,000x g and discard supernatant
- Repeat resuspension and centrifuge step
- Resuspend cell pellet with 50 uL or 100 uL 300 mM sucrose in water solution
