Team:Georgia State/notebookdata.html

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Monday, May 1st.

Sunday, June 1st.

Monday, June 2nd.

Tuesday, June 3rd.

Wednesday, June 5th.

Thursday, June 5th.

Friday, June 6th.

Saturday, June 7th.

Sunday, June 8th.

Monday, June 9th.

Tuesday, June 10th.

  • Revision of the protocols for next month’s lab-work, 12:00.
    • Everyone.
  • Revision of human practice ideas and collaborations with other teams, 13:00.
    • Everyone.

Wednesday, June 11th.

  • Electro-competent cells, 13:00.
    • Cinthya

Thursday, June 12th.

  • Sponsorships, all day.
    • Cinthya.

Friday, June 13th.

  • Meeting with advisors, 17:00.
  • Calcium-competent cells, 13:00.
    • Cinthya.

Saturday, June 14th.

Sunday, June 15th.

Monday, June 16th.

  • Electro-competent cells, 14:00.
    • Cinthya, Mercedes.
  • Minipreps, 16:00.
    • Cinthya.
  • Electrophoresis, 19:00.
    • Cinthya.
  • Inoculations, 20:00.
    • Mercedes.

Tuesday, June 17th.

Wednesday, June 18th.

  • Electro-competent cells, 10:00.
    • Cinthya.
  • Minipreps, 13:00.
    • Mercedes.
  • Electrophoresis, 16:00.
    • Cinthya.
  • Inoculations, 20:00.
    • Cinthya.

Thursday, June 19th.

Friday, June 20th.

  • Meeting with advisors, 17:00.

Saturday, June 21st.

Sunday, June 22nd.

Monday, June 23rd.

  • Calcium-competent cells, 12:00.
    • Cinthya.

Tuesday, June 24th.

  • Minipreps, 11:00
    • Mercedes.
  • Electrophoresis, 14:00.
    • Mercedes.
  • Inoculations. 18:00.
    • Mercedes.

Wednesday, June 25nd.

Thursday, June 26th.

  • Minipreps, 10:00
    • Mercedes.
  • Electrophoresis, 13:30.
    • Mercedes.

Friday, June 27th.

  • Meeting with advisors, 17:00.

Saturday, June 28nd.

Sunday, June 29nd.

Monday, June 30nd.

Tuesday, July 1st.

Wednesday, July 2nd.

Thursday, July 3rd.

Friday, July 4th.

  • Minipreps, 12:00.
    • Mercedes.
  • Practice: Ligations, 15:00.
    • Eduardo Ramirez (1).
  • Practice: Electrophoresis, 17:00.
    • Eduardo Ramirez (1)

Wednesday, July 5th.

Thursday, July 6th.

Friday, July 7th.

  • Tori Radcliff
      • Objective:To determine if Alkaline Phosphotase will bind to nitrocellulose paper and produce a signal when mixed with BCIP.
      Materials:Cut small strips of nitrocellulose paper (Nitran), Promega Alkaline Phosphatase (Conc: 1ng/ul), Alkaline 10X buffer diluted, BCIP
      Equipment:UV-Crosslinker
      Results: The Ap spread outward but stayed confined meaning the crosslinking worked. Adding the BCIP solution to the tube with the paper produced a noticeable discoloration. Adding the enzyme to the BCIP only produced a small discoloration. The binding of the alkaline phosphatase and BCIP to the paper was successful. The reaction worked only in the intended areas meaning that if the AP is bound to the factor c it may produce a signal only if it is cut, the AP nor BCIP disperses in the solution after being bound to the paper.

    Tuesday, July 8th.

    Wednesday, July 9th.

    Thursday, July 10th.

    • Practice: Transformation of calcium-competent cells, 11:00,
      • Eduardo Ramirez (1), Claudia.
    • Inoculation on LB-agar plates, 13:00.
      • Claudia.
    • Laboratory summer cleaning, 15:00.
      • Claudia.

    Friday, July 11th.

    Saturday, July 12th.

    Sunday, July 13th.

    Monday, July 14th.

    Tuesday, July 15th.

    • Practice: taking DNA out of the iGEM plates, 13:00.
      • Claudia.
    • Transformation with calcium-competent cells, 13:30.
      • Claudia.
    • Inoculations, 15:00.
      • Claudia.

    Wednesday, July 16th.

    • Minipreps, 12:00.
      • Eduardo Ramirez (1).
    • Ligations, 15:00.
      • Eduardo Ramirez (1).
    • Electrophoresis, 18:00.
    • Practice: DNA recovery from gel, 20:00.
      • Eduardo Ramirez (1)

    Thursday, July 17th.

    Friday, July 18th.

    Saturday, July 19th.

    • Meeting with advisors to talk about gene problems and doubts, 17:00.

    Sunday, July 20th.

    Monday, July 21st.

    Tuesday, July 22nd.

    • Minipreps, 11:00.
      • Claudia.
    • Ligations, 15:00.
      • Claudia.
    • Electrophoresis, 19:00.
      • Claudia
    • Practice: DNA recovery from gel, 21:00.
      • Claudia.

    Wednesday, July 23rd.

    Thursday, July 24th.

    Friday, July 25th.

    Saturday, July 26th.

    • Meeting with advisors, 17:00.

     

    Monday, July 29th.

    • Meeting to plan the semester’s activities, 14:00.
      • Schedule of people in the lab.
      • Ways to integrate other students.
      • Events to talk about iGEM.

    Sunday, July 27th.

    Monday, July 28th.

    Tuesday, July 29th.

    Wednesday, July 30th.

    Thursday, July 31st.

    Tuesday,August 1st.

  • Tori Radcliff
      • Objective:Before the fusion step, the hCG needs to be placed into the PSB1C3 plasmid, so that the hCG can be submitted. In order to add the hCG to add to the plasmid restriction sites need to be added this was done by adding the prefix and suffix through PCR.
      Materials:Qiagen Master mix (2x), Prefix Primer, Suffix Primer, hCG DNA (diluted to 10ng/ul), and Nuclease free H20
      Equipment:Thermocycler
      Results:
      Simulated gel using SnapGene1% Agarose gel, experimental results

    Wednesday, August 2nd.

    Thursday, August 3rd.

  • Tori Radcliff
      • Objective:To double digest both the PSB1C3 backbone and our hCG PCR product
      Materials:DNA of ECFP-PCB1C3 (102 ng/ul) and hCG PCR product, Restriction enzymes: NotI, EcoRI, XbaI, PstI,SpeI, Green Fastdigest Buffer (10x), and Nuclease free H20
      Equipment:Thermocycler
      Results:
      Simulated gel using SnapGeneThe Wells Contain the following:
    • 1. 1Kb New England Biolabs Ladder
    • 2. hCG uncut
    • 3. hCG cut with EcoR1 and Pst1
    • 4. hCG cut with Xba1 and Spe1
    • 5. ECFP cut with EcoR1 and Pst1
    • 6. ECFP cut with Xba1 and Spe1

    Friday August 4th.

  • Cara Jones
      • Objective: Clean used GST resin so that it may be used in future protein purifications.
      Materials: GST Resin, 70% Ethanol, GST Elution Buffer
      Equipment: Centrifuge,Vortexer, Micropipette,Eppendorf tubes/Falcon tubes, Spectrophotometer, and Cuvettes

    Saturday, August 5th.

  • Tori Radcliff
      • Objective:To repeat the double digest of PSCB1C3 and hCG PCR
      Materials:DNA of Lt10-PCB1C3 (80 ng/ul) and hCG PCR product, Restriction enzymes: NotI, EcoRI, XbaI, PstI,SpeI, Green Fastdigest Buffer (10x), and Nuclease free H20
      Equipment:Thermocycler
      Results:
      Since the diagnostic gel was a success the PSB1C backbone was extracted from the Lt 10 plasmid using an E-gel.

    Sunday, August 6th.

    Monday, August 7th.

    Tuesday, August 8th.

    Wednesday, August 9th.

  • Tori Radcliff
      • Ligation of hCG to PSB1C3

    Thursday, August 10

  • Tori Radcliff
      • Transformation of hCG-PSB1C3 ligation and overnight culture

    Friday, August 11

  • Tori Radcliff
      • Colony PCR of hCG-PSB1C3 colonies

    Saturday, August 12

    Sunday, August 13

  • Tori Radcliff
      • Overnight culture of hCG-PSB1C3 colonies

    Monday, August 14

  • Tori Radcliff
      • Miniprep of hCG-PSB1C3 cultures
      Diagnostic restriction digest
      PCR of factor-C gBlocks
        test

    Friday, August 15

    Saturday, August 16

    • Miniprep GFP, 12:00.
      • Mercedes.
    • Digestions, 15:30.
      • Mercedes.

    Sunday, August 17

    • Preparation of TAE buffer, 18:00.
      • Mercedes.
    • Digestions, 20:00.
      • Mercedes.
    • Electrophoresis, 22:00.
      • Mercedes, Cesar, Claudia, Eduardo Ramirez (2), Omar.

    Monday, August 18th.

    • Electrophoresis, 14:00.
      • Minerva.
    • Teaching of the newbies (Miniprep basics), 16:00.
      • Mercedes.
    • Autoclave of general materials, 18:00.
      • Everyone.

    Tuesday, August 19

    • MiniPrep GFP, 12:30
      • Mercedes
    • Digestions, 18:00
      • Eduardo Cepeda
    • Electrophoresis, 21:00
      • Newbies (Alex, Dany).

    Wednesday, August 20

    • iGEM talk with interested students, 16:30.
      • Special Guest: Ana Sifuentes
    • Teaching of how to make media to newbies, 18:00.
      • Claudia.

    Thursday, August 21

    • Teaching of how to make an electrophoresis gel to the newbies, 18:00.
      • Claudia.

    Friday, August 22

    • Teaching of how to make a transformation in calcium-competent cells, 17:00.
      • Claudia.
    • Purification of the Minipreps of GFP and the promoters D2 and D3, 18:00.
      • Eduardo Ramirez (2), Omar.
    • Electrophoresis of the past purifications, 22:00.
      • Mercedes.
    • Purification of the electrophoresis and ligations, 23:30.
      • Mercedes, Eduardo Cepeda.

    Saturday, August 23

    • Making of electro-competent cells, 9:00.
      • Mercedes.
    • Visit to UANL to meet their iGEM team and to hear the iGEM HQ talk, 13:30.
      • Mercedes, Claudia, Eduardo Ramirez (1), Eduardo Cepeda.
    • Transformations of the electro-competent cells with D2, D3 and GFP, 18:00.
      • Claudia.
    • Inoculation of the ligation D2-GFP, 20:00.
      • Eduardo Cepeda.

    Sunday, August 24

    • Miniprep of the ligation D2-GFP, 11:00.
      • Minerva.
    • Digestion of D2, D3, GFP and RFP, 16:00.
      • Minerva, Eduardo Cepeda.
    • Electrophoresis, 19:00.
      • Minerva, Eduardo Cepeda.
    • DNA purification of the gel, 20:15.
      • Mercedes.
    • Ligations D2-GFP, D3-GFP, 21:00.
      • Mercedes.

    Monday, August 25

    • Miniprep D2-GFP, 13:00.
      • Eduardo Cepeda.
    • Digestion of D2, 16:00.
      • Eduardo Cepeda.
    • Inoculation practice with the newbies, 19:00.
      • Claudia.
    • Transformations with electro-competent cells, 21:30.
      • Cesar.
    • Preparation of plaques, 23:00.
      • Eduardo Cepeda.

    Tuesday, August 26

    Wednesday, August 27

    • Inoculm of BL21 7:00.
      • Eduardo Cepeda.
    • Miniprep D2-GFP, D3-GFP, D2-RFP, D3-RFP, 13:30.
      • Eduardo Cepeda.
    • Digestions, 18:00.
      • Mercedes.
    • Electrophoresis, 20:00
      • Mercedes.
    • Preparation of calcium-competents BL21,
      • Claudia, Eduardo Ramirez.

    Thursday, August 28

    • Electrophoresis, 22:00.
      • Newbies.
    • Preparation of calcium-competent cells TOP10, 22:00.
      • Eduardo Ramirez (2).

    Saturday, August 29th.

  • Tori Radcliff
      • Objective:To transform the ligation of hCG and PSB1C3
      Materials:C2987I NEB competent cells, ligation reaction
      Equipment:shaker and incubator
      Results:
      Since the diagnostic gel was a success the PSB1C backbone was extracted from the Lt 10 plasmid using an E-gel.

    Saturday, August 30

    Sunday, August 31

    • Miniprep GFP Kn, RFP, 3 replicas of GFP Cam, 15:00.
      • Claudia, Eduardo Ramirez (2).
    • Digestions, 18:30.
      • Mercedes.
    • Electrophoresis, 22:40.
      • Omar.

    Monday, September 1st.

    • Apoptosis kit arrived!, 14:00.
    • Accutase arrived!, 14:00.
    • Genes arrived!, 14:00.
    • Preparation of LB and LB-agar media, 13:30.
      • Eduardo Ramirez (1).
    • Preparation of the plaques for the transformation, 17:00.
      • Eduardo Cepeda.

    Abbreviation

    Construct

    Gene

    Resistance

    LPP

    C1

    LPP Red Lambda

    Amp

    MSBB

    C2

    Red Lambda

    Kn + Amp

    H-GFP

    C4

    GFP

    Amp

    C-Apop

    C5

    Apoptine

    Amp

    H-SiRNA 1

    C6

    SiRNA 1

    Amp

    H-SiRNA 2

    C7

    SiRNA 2

    Amp

    H-SiRNA 3

    C8

    SiRNA 3

    Amp

    H-SiRNA-Apop

    C9

    SiRNA + Apoptine

    Amp

    BBa_1

    Biobrick

    		  

    Cam

    BBa_2

    Biobrick

    		  

    Cam

    BBa_3

    Biobrick

    Peptide + p3

    Cam
    • HyperLadder 50bp arrived!, 17:10
    • HLA arrived!, 17:10:
    • Transformation of C1 through C7 in TOP10, 19:00.
      • Minerva.
    • Transformation of GFP and RFP in TOP10, 21:30.
      • Mercedes.

    Tuesday, September 2

    • Inoculation of plates with transformed colonies from yesterday, 12:00.
      • Minerva.
    • Transformations of GFP Amp, RFP Cam, D1 Kn, D2 Cam, D3 Amp, 19:30.
      • Eduardo Ramirez (2).
    • Miniprep of the grown transformed cells from yesterday (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 21:00.
      • Eduardo Cepeda.

    Wednesday, September 3

    • Digestions of the last minipreps, 02:00.
      • Eduardo Cepeda.
    • Electrophoresis, 03:30.
      • Eduardo Cepeda.
    • Preparation of electro-competent TOP10 cells, 08:00.
      • Newbies: Alex, Danny, Chuy, Caro.
    • Preparation of stock cells (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 18:30.
      • Mercedes.
    • Ligations of the C4, C5, C6, C7 with the pcb1c3, 20:30.
      • Eduardo Cepeda.
    • Inoculation of TOP10 for electro-competent cells tomorrow and D2 cells, 01:25.
      • Mercedes.

    Thursday, September 4

    Friday, September 5

    • Preparation of the tickets for the cake raffle (fundraising), 12:00.
      • Cinthya, Omar, Eduardo Ramirez (1), Eduardo Cepeda, Mercedes.
    • Miniprep, 14:00.
      • Mercedes and Eduardo Cepeda with special guest: Ana Cristina.
    • Talk with Professor Cardineau about the legislation proposal, 17:00.
      • Omar.
    • Preparations of electro-competente TOP10 cells, 17:30.
      • Eduardo Ramirez (2), Mercedes.
    • Digestions, 19:00.
      • Eduardo Cepeda.
    • Inoculation of LB-agar plaques, 21:00.
      • Cynthia.

    Saturday, September 6

    Sunday, September 7

    Monday, September 8

    Tuesday, September 9

    • Inoculation of PCB1c3 (with C4, C5, C7 separately), 13:30.
      • Eduardo Cepeda.

    Wednesday, September 10

    Thursday, September 11

    • Revision of (not grown yet) plates, 12:00.
      • Eduardo Cepeda.
    • Inoculaton of D2-GFP, 21:00.
      • Eduardo Cepeda.

    Friday, September 12th

    • Cleaning of the electroporation cells for the electro-competent cells, 16:00.
      • Minerva.
    • Inoculation of cells that grew from the prior day, 18:00.
      • Cinthya.

    Saturday, September 13

    • Transformation of the D2 and D3 variants for the interlab project, 00:10.
      • Eduardo Ramirez (2), Mercedes, Claudia, Omar.

    Sunday, September 14th.

    • Miniprep, 17:00.
      • Mercedes, Eduardo Ramirez (2)
    • Sterilization of Falcon tubes, LB broth and plastic pearls, 18:30.
      • Omar
    • Digestions, 21:00.
      • Mercedes.
    • Inoculation of the transformed cells with D2 and D3 variants, 22:00.
      • Omar.

    Monday, September 15

    • Miniprep of yesterday's inoculations of D2 and D3 variants, 10:00.
      • Mercedes.
    • Digestions, 17:30.
      • Eduardo Ramirez (2).
    • Inoculation of TOP10 and XL-Blue (Tet), 22:50.
      • Mercedes.

    Tuesday, September 16th.

    • Transformations of the M13 bacteriophage into the XL-Blue, 10:00.
      • Mercedes.

    Wednesday, September 17

    • Miniprep 01:15.
      • Minerva, Eduardo Cepeda.
    • Digestions (C4, C5, C6, C7 and , 02:53.
      • Eduardo Cepeda.
    • PBS buffer, 12:30.
      • Omar.
    • Recovery of DNA from Gel, 13:30.
      • Eduardo Cepeda.

    Thursday, September 18

    • Transformations of C8, C9 and ligation in TOP10 electro-competent, 00:40.
      • Cinthya, Omar.
    • Electrophoresis, 01:30.
      • Mercedes, Eduardo Ramirez (1).
    • Plates from the transformations, 01:50.
      • Omar.
    • Miniprep of PET, 12:30.
      • Eduardo Cepeda.
    • Transformation replica of the same ligation as before, 15:30.
      • Omar.
    • Preparation of LB broth, 17:00.
      • All the newbies (Caro, Jesus, Alex, Dany).
    • Digestion of PET, 19:30.
      • Claudia.

    Friday, September 19

    • Ligation of PET with all the constructs, 01:19.
      • Eduardo Cepeda.
    • Electrophoresis, 03:30.
      • Eduardo Cepeda.
    • Miniprep, 8:30.
      • Mercedes.
    • Digestions, 13:00.
      • Mercedes.
    • Electrophoresis, 15:30.
      • Mercedes.
    • Quantification of the fluorescence for the interlab project, 16:30.
      • Mercedes, Eduardo Ramirez (2).
    • Group photo taken, 18:00.
      • Everyone except Mercedes, Eduardo Cepeda and Eduardo Ramirez.
    • Group video filmed, 18:30.
      • Everyone.
    • Transformation of TOP10 cells with PET + (C4, C5, C6, C7), 20:30.
      • Cinthya, Eduardo Cepeda.
    • Transformation of TOP10 cells with various repetitions of D2 and D3 (with GFP or RFP), 21:00.
      • Mercedes, Eduardo Ramirez (2).
    • Inoculation in Falcon tubes with the ligation of C5 and various others, 22:00.
      • Omar.
    • Inoculation in plates of the previous transformed TOP10 cells with PET, 23:00.
      • Cinthya.

    Saturday, September 20

    • Transformation in electro-competent cells, 01:20.
      • Mercedes, Eduardo Ramirez.
    • Minipreps, 18:00.
      • Mercedes, Omar.
    • Digestions, 22:00.

    Sunday, September 21st.

    • Electrophoresis, 00:00.
      • Mercedes.
    • Inoculations for minipreps, 01:00.
      • Mercedes.
    • Minipreps,
      • Eduardo Cepeda.

    Monday, September 22

    • Miniprep, 08:00.
      • Mercedes.
    • Revision of the cell stocks, contamination detected, 09:00.
      • Mercedes.
    • Inoculations of C8 and C9, 22:00.
      • Eduardo Cepeda.

    Tuesday, September 23

    • Preparation of electo-competent cells DH5a, 03:00.
      • Minerva.
    • Minipreps for the interlab, 06:00
      • Mercedes, Minerva.
    • Transformations for the interlab, 07:30.
      • Mercedes, Minerva.
    • Digestions for the interlab, 12:30.
      • Mercedes.
    • Miniprep, 13:30.
      • Eduardo Cepeda.
    • Electrophoresis for interlab, 19:00.
      • Eduardo Ramirez (2)
    • Electrophoresis for the project (C8, C9), 21:00.
      • Eduardo Ramirez (2)

    Wednesday, September 24

    • Purification of the electrophoresis gel, 01:20.
      • Mercedes.
    • Digestions for the interlab, 01:20.
      • Eduardo Cepeda.
    • Ligations, 02:00.
      • Eduardo Cepeda.
    • Electrophoresis for the interlab, 02:40.
      • Mercedes.
    • Inoculations for the interlab, 11:00.
      • Omar, Mercedes.

    Thursday, September 25

    • Inoculation of XL-Blue, 22:00.
      • Mercedes.
    • Infection of the XL-Blue with bacteriophages, 23:00.
      • Mercedes.

    Friday, September 26

    • Miniprep of the bacteriophage-infected cells, 03:00.
      • Mercedes.
    • Preparation of the cells for quantification of fluorescence and OD for the interlab project, from 8:00 to 24:00.
      • Mercedes.
    • Purification of the miniprep. 18:00.
      • Eduardo Ramirez (1), Claudia.
    • Transformations of C1, C2, Bba_1, Bba_2, and ligations, 19:00.
      • Cinthya, Omar.
    • Plating of the transformations, 22:00.
      • Cinthya.

    Saturday, September 27

    • Inoculation of C1, C2, Bba_1, Bba _2 and ligations, 20:00.
      • Omar
    • Sterilization of material, 22:00.
      • Omar.

    Sunday, September 28

    • Preparation of electro-competent cells, 01:00.
      • Eduardo Cepeda.
    • Ligations, 03:00.
      • Eduardo Cepeda.
    • Electrophoresis, 12:00.
      • Eduardo Cepeda.
    • Minipreps of the inoculations from Saturday, 13:00.
      • Claudia, Eduardo Ramirez (1).
    • Recovery of DNA from gel, 15:00.
      • Claudia.

    Monday, September 29

    Tuesday, September 30

    Wednesday, October 1st.

    • Minipreps of all constructs, 12:00.
      • Eduardo.
    • Digestions of all constructs, 20:30.
      • Claudia, Jesus.
    • Miniprep of C2, 21:00.
      • Jesus.

    Thursday, October 2nd.

    • Collaboration: gave C4 through C9 to the UANL team, 13:30.
      • Mercedes.
    • Inoculo XL-Blue, 18:00.
      • Eduardo Ramirez (2).
    • Miniprep C8, C10, Bb_1, C6, 16:30.
      • Eduardo Cepeda, Eduardo Ramirez (2).
    • Miniprep of phagemides, 18:30.
      • Eduardo Cepeda.

    Friday, October 3rd.

    • Digestions of C1, C2 and PET variants of the other constructs, 12:00.
      • Eduardo Cepeda.
    • Minipreps, 12:00.
      • Claudia.
    • Electrophoresis, 16:00.
      • Eduardo Cepeda.
    • Inoculations, 20:00.
      • Mercedes.

    Saturday, October 4th.

    Sunday, October 5th.

    • Western Blot, 01:00.
      • Minerva.
    • Minipreps, 00:30.
      • Eduardo Cepeda.
    • Electrophoresis, 04:00.
      • Eduardo Cepeda.
    • Inoculations, 18:00.
      • Eduardo Cepeda.

    Monday, October 6th.

    Tuesday, October 7th.

    Wednesday, October 8th.

    Thursday, October 9th.

    • Minipreps for cells with flippase, 12:00.
      • Eduardo Cepeda.
    • Stock of cells with flippase, 12:00.
      • Mercedes.
    • Digestions for flippase, 16:00.
      • Eduardo Cepeda.
    • Electrophoresis, 18:00.
      • Eduardo Cepeda.

    Friday, October 10th.

    • Digestions of Bba_1, Bba_2 and Bba_3, 08:00.
      • Eduardo Cepeda.
    • Digestion of the modified phage, 09:00.
      • Mercedes.
    • Electrophoresis, 12:00.
      • Eduardo Ramirez (2).
    • Ligation of the phages back to circle form, 13:00.
      • Mercedes.

    Saturday, October 11th.

    • Inoculations for constructs stocks, 20:00.
      • Mercedes.

    Sunday, October 12th.

    • Pictures taken for the wiki, 13:00.
      • Everyone.
    • PCR, 16:00.
      • Eduardo Cepeda.
    • Stocks, 18:00.
      • Mercedes.
    • Electrophoresis, 20:00.
      • Eduardo Cepeda.

    Monday, October 13th.

    • PCR for Red Lambda confirmation, 13:00.
      • Eduardo Cepeda.
    • Electrophoresis, 15:00.
      • Mercedes.
    • Digestions, 16:00.
      • Mercedes.
    • TAE buffer preparation, 20:00.
      • Newbies: Alejandro, Danny.
    • Western Blott, 21:00.
      • Minerva, Claudia.

    Tuesday, October 14th.

    • Work on wiki
      • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez

    Wednesday, October 15th.

    • Work on wiki
      • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez

    Thursday, October 16th.

    • Work on wiki
      • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez

    Friday, October 17th.

    • Work on wiki, its wiki-freeze day!!!
      • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez

    Retrieved from "https://2014.igem.org/Team:Tec-Monterrey/ITESM14_notebook_data.html"