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Revision as of 12:17, 10 August 2017

Discription

  • This year our project is about using light to control the reproduction of E.coli.
  • We decide to block the replication of E.coli by using dCas9 targeting at OriC.
  • By splitting dCas9 into two parts, each separately fused with a light inducible dimer.
  • nder the irradiation of light, the dimer will dimerize, promoting the function of dCas9.
  • Then the dCas9 will block the OriC, the replication origin of E.coli.
  • After turning off the light, the dCas9 will split again, which causes the E.coli to reproduce again.