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.logo{ | .logo{ | ||
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<a href="file:///C:/Users/hp/Desktop/%E5%AF%BC%E8%88%AA%E6%A0%8F%EF%BC%88%E4%B8%8A%E8%BE%B9%E7%95%8C%E6%82%AC%E6%B5%AE%E5%BC%8F%EF%BC%89/%E4%B8%8B%E6%8B%89%E5%BC%8F%E5%AF%BC%E8%88%AA%E6%A0%8F%EF%BC%88%E5%BE%85%E5%AE%9A%EF%BC%89.html" class="logo" title="logo"><img src="https://static.igem.org/mediawiki/2017/3/3d/HZAU-logo.png" width="10%"></a> | <a href="file:///C:/Users/hp/Desktop/%E5%AF%BC%E8%88%AA%E6%A0%8F%EF%BC%88%E4%B8%8A%E8%BE%B9%E7%95%8C%E6%82%AC%E6%B5%AE%E5%BC%8F%EF%BC%89/%E4%B8%8B%E6%8B%89%E5%BC%8F%E5%AF%BC%E8%88%AA%E6%A0%8F%EF%BC%88%E5%BE%85%E5%AE%9A%EF%BC%89.html" class="logo" title="logo"><img src="https://static.igem.org/mediawiki/2017/3/3d/HZAU-logo.png" width="10%"></a> | ||
<a href="#" class="Go_Top" title="Go_Top"><img src="https://static.igem.org/mediawiki/2015/2/2a/Igemlogo_banner.jpg" width="100px" height="100px"></a> | <a href="#" class="Go_Top" title="Go_Top"><img src="https://static.igem.org/mediawiki/2015/2/2a/Igemlogo_banner.jpg" width="100px" height="100px"></a> | ||
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<li>nder the irradiation of light, the dimer will dimerize, promoting the function of dCas9.</li> | <li>nder the irradiation of light, the dimer will dimerize, promoting the function of dCas9.</li> | ||
<li>Then the dCas9 will block the OriC, the replication origin of E.coli.</li> | <li>Then the dCas9 will block the OriC, the replication origin of E.coli.</li> | ||
− | <li>After turning off the light, the dCas9 will split again, which causes the E.coli to reproduce again. | + | <li>After turning off the light, the dCas9 will split again, which causes the E.coli to reproduce again.</li> |
</ul> | </ul> | ||
− | + | </div> | |
</body> | </body> | ||
</html> | </html> |
Revision as of 08:59, 10 August 2017
Discription
- This year our project is about using light to control the reproduction of E.coli.
- We decide to block the replication of E.coli by using dCas9 targeting at OriC.
- By splitting dCas9 into two parts, each separately fused with a light inducible dimer.
- nder the irradiation of light, the dimer will dimerize, promoting the function of dCas9.
- Then the dCas9 will block the OriC, the replication origin of E.coli.
- After turning off the light, the dCas9 will split again, which causes the E.coli to reproduce again.