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<a href="#" class="Go_Top" title="Go_Top"><img src="https://static.igem.org/mediawiki/2015/2/2a/Igemlogo_banner.jpg" width="100px" height="100px"></a> | <a href="#" class="Go_Top" title="Go_Top"><img src="https://static.igem.org/mediawiki/2015/2/2a/Igemlogo_banner.jpg" width="100px" height="100px"></a> |
Revision as of 15:01, 10 August 2017
Discription
- This year our project is about using light to control the reproduction of E.coli.
- We decide to block the replication of E.coli by using dCas9 targeting at OriC.
- By splitting dCas9 into two parts, each separately fused with a light inducible dimer.
- nder the irradiation of light, the dimer will dimerize, promoting the function of dCas9.
- Then the dCas9 will block the OriC, the replication origin of E.coli.
- After turning off the light, the dCas9 will split again, which causes the E.coli to reproduce again.