Team:HZAU-China

Discription

• This year our project is about using light to control the reproduction of E.coli.
• We decide to block the replication of E.coli by using dCas9 targeting at OriC.
• By splitting dCas9 into two parts, each separately fused with a light inducible dimer.
• nder the irradiation of light, the dimer will dimerize, promoting the function of dCas9.
• Then the dCas9 will block the OriC, the replication origin of E.coli.
• After turning off the light, the dCas9 will split again, which causes the E.coli to reproduce again.