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| − | <!DOCTYPE html>
| + | {Template:HZAU-China} |
| − | <html lang="en">
| + | |
| − | | + | |
| − | <head>
| + | |
| − | <meta charset="UTF-8">
| + | |
| − | <!--一些初始设置-->
| + | |
| − | <style type="text/css">
| + | |
| − | /* HTML5 display-role reset for older browsers */
| + | |
| − | | + | |
| − | article,
| + | |
| − | aside,
| + | |
| − | details,
| + | |
| − | figcaption,
| + | |
| − | figure,
| + | |
| − | footer,
| + | |
| − | header,
| + | |
| − | hgroup,
| + | |
| − | nav,
| + | |
| − | section {
| + | |
| − | display: block;
| + | |
| − | }
| + | |
| − | | + | |
| − | ol,
| + | |
| − | ul {
| + | |
| − | list-style: none;
| + | |
| − | }
| + | |
| − | | + | |
| − | blockquote,
| + | |
| − | q {
| + | |
| − | quotes: none;
| + | |
| − | }
| + | |
| − | | + | |
| − | blockquote:before,
| + | |
| − | blockquote:after,
| + | |
| − | q:before,
| + | |
| − | q:after {
| + | |
| − | content: '';
| + | |
| − | content: none;
| + | |
| − | }
| + | |
| − | | + | |
| − | table {
| + | |
| − | border-collapse: collapse;
| + | |
| − | border-spacing: 0;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | <!-- HZAU-navigation's style -->
| + | |
| − | <style type="text/css">
| + | |
| − | div.nav_body {
| + | |
| − | position: fixed;
| + | |
| − | padding: 0px;
| + | |
| − | left: 0px;
| + | |
| − | top: 16px;
| + | |
| − | width: 100%;
| + | |
| − | min-width: 950px;
| + | |
| − | height: 36px;
| + | |
| − | box-shadow: 0px 1px 3px #aaaaaa;
| + | |
| − | z-index: 1;
| + | |
| − | color: rgb(249, 228, 137);
| + | |
| − | text-decoration-line: none;
| + | |
| − | font-family: "Arial", Helvetica, sans-serif;
| + | |
| − | background-color: #000;
| + | |
| − | z-index: 99;
| + | |
| − | }
| + | |
| − | | + | |
| − | ul#nav_organ {
| + | |
| − | float: right;
| + | |
| − | list-style: none;
| + | |
| − | margin: 6px auto;
| + | |
| − | text-decoration-line: none;
| + | |
| − | font-family: "Arial", Helvetica, sans-serif;
| + | |
| − | color: rgb(249, 228, 137);
| + | |
| − | background-color: #000;
| + | |
| − | }
| + | |
| − | | + | |
| − | ul#nav_organ li {
| + | |
| − | display: block;
| + | |
| − | float: left;
| + | |
| − | }
| + | |
| − | | + | |
| − | a.nav_tissue {
| + | |
| − | padding: 8px 15px 8px 15px;
| + | |
| − | line-height: 20px;
| + | |
| − | text-decoration-line: none;
| + | |
| − | font-family: "Arial", Helvetica, sans-serif;
| + | |
| − | color: rgb(249, 228, 137);
| + | |
| − | font-size: 16px;
| + | |
| − | }
| + | |
| − | | + | |
| − | span.caret {
| + | |
| − | display: inline-block;
| + | |
| − | margin-left: 2px;
| + | |
| − | vertical-align: middle;
| + | |
| − | width: 0;
| + | |
| − | height: 0;
| + | |
| − | border-left: 4px solid transparent;
| + | |
| − | border-right: 4px solid transparent;
| + | |
| − | border-top: 4px solid rgb(249, 228, 137);
| + | |
| − | }
| + | |
| − | | + | |
| − | .nav_cell {
| + | |
| − | visibility: hidden;
| + | |
| − | opacity: 0;
| + | |
| − | display: block;
| + | |
| − | box-sizing: border-box;
| + | |
| − | left: auto;
| + | |
| − | position: absolute;
| + | |
| − | top: 30px;
| + | |
| − | z-index: 1000;
| + | |
| − | float: left;
| + | |
| − | min-width: 160px;
| + | |
| − | margin: 0 0 0 -4px;
| + | |
| − | font-size: 14px;
| + | |
| − | text-align: left;
| + | |
| − | list-style: none;
| + | |
| − | color: rgb(249, 228, 137);
| + | |
| − | background-color: #000;
| + | |
| − | background-clip: padding-box;
| + | |
| − | border: 1px solid rgba(0, 0, 0, .15);
| + | |
| − | border-radius: 4px;
| + | |
| − | box-shadow: 0 6px 12px rgba(0, 0, 0, .175);
| + | |
| − | -webkit-transition: opacity 0.4s linear;
| + | |
| − | -moz-transition: opacity 0.4s linear;
| + | |
| − | -ms-transition: opacity 0.4s linear;
| + | |
| − | -o-transition: opacity 0.4s linear;
| + | |
| − | transition: opacity 0.4s linear;
| + | |
| − | }
| + | |
| − | | + | |
| − | .mw-content-ltr ul,
| + | |
| − | .mw-content-rtl .mw-content-ltr ul {
| + | |
| − | margin: 0 0 0 -4px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .shadow {
| + | |
| − | box-shadow: 0px 1px 3px rgb(249, 228, 137);
| + | |
| − | }
| + | |
| − | | + | |
| − | .nav_cell>li {
| + | |
| − | display: block;
| + | |
| − | float: left;
| + | |
| − | clear: both;
| + | |
| − | height: 30px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .nav_cell>li>a {
| + | |
| − | padding-top: 3px;
| + | |
| − | padding-bottom: 3px;
| + | |
| − | padding-left: 20px;
| + | |
| − | padding-right: 20px;
| + | |
| − | text-decoration-line: none;
| + | |
| − | color: rgb(249, 228, 137);
| + | |
| − | }
| + | |
| − | | + | |
| − | .menu_item:hover .nav_cell {
| + | |
| − | visibility: visible;
| + | |
| − | opacity: 1;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | <!--消除一些不兼容引起的错拍-->
| + | |
| − | <style type="text/css">
| + | |
| − | .ppp {
| + | |
| − | display: block;
| + | |
| − | padding: 100px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #sideMenu,
| + | |
| − | #top_title,
| + | |
| − | #team_submenu {
| + | |
| − | display: none;
| + | |
| − | }
| + | |
| − | | + | |
| − | #content {
| + | |
| − | padding: 0px;
| + | |
| − | width: 100%;
| + | |
| − | margin-top: 0px;
| + | |
| − | margin-left: 0px;
| + | |
| − | margin: 0 0 0 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | #bodyContent h1,
| + | |
| − | #bodyContent h2,
| + | |
| − | #bodyContent h3,
| + | |
| − | #bodyContent h4,
| + | |
| − | #bodyContent h5 {
| + | |
| − | margin-bottom: 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #team_name {
| + | |
| − | display: none
| + | |
| − | }
| + | |
| − | | + | |
| − | .global_wrapper {
| + | |
| − | padding: 0px 0px 0px 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #top_menu_14 {
| + | |
| − | height: 16px;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | <!--内容的样式-->
| + | |
| − | <style>
| + | |
| − | body {
| + | |
| − | font-family: "Arial", Helvetica, sans-serif;
| + | |
| − | }
| + | |
| − | | + | |
| − | .HZAU_content {
| + | |
| − | position: relative;
| + | |
| − | right: 0px;
| + | |
| − | width: 1000px;
| + | |
| − | height: 3000px;
| + | |
| − | margin: 0 0 0 250px;
| + | |
| − | z-index: -1;
| + | |
| − | }
| + | |
| − | | + | |
| − | .HZAU_2017_menu {
| + | |
| − | display: block;
| + | |
| − | position: fixed;
| + | |
| − | left: 15px;
| + | |
| − | top: 100px;
| + | |
| − | height: 337px;
| + | |
| − | width: 233px;
| + | |
| − | overflow: hidden;
| + | |
| − | }
| + | |
| − | | + | |
| − | .beijin {
| + | |
| − | height: 337px;
| + | |
| − | width: 233px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .HZAU_2017_menu ul {
| + | |
| − | position: absolute;
| + | |
| − | top: 50%;
| + | |
| − | margin-top: -80px;
| + | |
| − | margin-left: 0px;
| + | |
| − | left: 0px;
| + | |
| − | width: 100%;
| + | |
| − | padding-left: 0px;
| + | |
| − | font-size: 16px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .HZAU_2017_menu li {
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | .HZAU_2017_menu li a {
| + | |
| − | display: inline-block;
| + | |
| − | width: 100%;
| + | |
| − | height: 100%;
| + | |
| − | padding: 20px 0px 20px 0px;
| + | |
| − | margin-left: 13px;
| + | |
| − | text-align: left;
| + | |
| − | color: rgb(249, 228, 137);
| + | |
| − | text-decoration: none;
| + | |
| − | font-style: arial;
| + | |
| − | line-height: 90%;
| + | |
| − | }
| + | |
| − | | + | |
| − | .HZAU_2017_menu li:hover {
| + | |
| − | background: rgb(249, 228, 137);
| + | |
| − | }
| + | |
| − | | + | |
| − | .HZAU_2017_menu li a:hover {
| + | |
| − | color: #000;
| + | |
| − | }
| + | |
| − | | + | |
| − | .item {
| + | |
| − | width: 100%;
| + | |
| − | padding-left: 40px;
| + | |
| − | | + | |
| − | | + | |
| − | }
| + | |
| − | | + | |
| − | .biaoti {
| + | |
| − | font-size: 50px;
| + | |
| − | font-weight: bold;
| + | |
| − | text-align: center;
| + | |
| − | width: 100%;
| + | |
| − | display: block;
| + | |
| − | padding-bottom: 50px;
| + | |
| − | padding-top: 50px;
| + | |
| − | color: rgb(63, 59, 58);
| + | |
| − | }
| + | |
| − | | + | |
| − | .zhengwen {
| + | |
| − | font-style: arial;
| + | |
| − | font-size: 24px;
| + | |
| − | line-height: 120%;
| + | |
| − | text-align: justify;
| + | |
| − | color: #000;
| + | |
| − | }
| + | |
| − | | + | |
| − | .tu_1 {
| + | |
| − | position: relative;
| + | |
| − | left: 450px;
| + | |
| − | padding-top: 30px;
| + | |
| − | padding-bottom: 30px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .tu_2 {
| + | |
| − | position: relative;
| + | |
| − | left: 450px;
| + | |
| − | padding-top: 30px;
| + | |
| − | padding-bottom: 30px;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | </head>
| + | |
| − | | + | |
| − | <body>
| + | |
| − | <div class="nav_body">
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China" class="HZAU-logo" title="logo">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/b/be/HZAU_2017_logo_1.png" height="100%">
| + | |
| − | </a>
| + | |
| − | <ul id="nav_organ">
| + | |
| − | <li class="Home">
| + | |
| − | <a class="nav_tissue" href="https://2017.igem.org/Team:HZAU-China">Home</a>
| + | |
| − | </li>
| + | |
| − | <li class="Team">
| + | |
| − | <a class="nav_tissue" href="https://2017.igem.org/Team:HZAU-China/Team">Team</a>
| + | |
| − | </li>
| + | |
| − | <li class="Project menu_item">
| + | |
| − | <a class="nav_tissue" href="">Project
| + | |
| − | <span class="caret"></span>
| + | |
| − | </a>
| + | |
| − | <ul class="nav_cell shadow">
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Description">Description</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Design">Design</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Experiments">Experiments</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Notebook">Notebook</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/InterLab">InterLab</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Model">Model</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Results">Results</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Demonstrate">Demonstrate</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Improve">Improve</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Project/Attributions">Attributions</a>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li class="Parts menu_item">
| + | |
| − | <a class="nav_tissue" href="">Parts
| + | |
| − | <span class="caret"></span>
| + | |
| − | </a>
| + | |
| − | <ul class="nav_cell shadow">
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Parts">Parts</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Basic Parts">Basic Parts</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Composite Parts">Composite Parts</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Part Collection">Part Collection</a>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li class="Safety">
| + | |
| − | <a class="nav_tissue" href="">Safety</a>
| + | |
| − | </li>
| + | |
| − | <li class="HP menu_item">
| + | |
| − | <a class="nav_tissue" href="">Human Practices
| + | |
| − | <span class="caret"></span>
| + | |
| − | </a>
| + | |
| − | <ul class="nav_cell shadow">
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Human Practices/Silver HP">Silver HP</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Human Practices/Integrated and Gold">Integrated and Gold</a>
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Human Practices/Public Engagement">Public Engagement</a>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li class="Awards menu_item">
| + | |
| − | <a class="nav_tissue" href="">Awards
| + | |
| − | <span class="caret"></span>
| + | |
| − | </a>
| + | |
| − | <ul class="nav_cell shadow">
| + | |
| − | <li>
| + | |
| − | <a href="https://2017.igem.org/Team:HZAU-China/Awards/Hardware">Hardware</a>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li class="Judging Form">
| + | |
| − | <a class="nav_tissue" href="https://2017.igem.org/Team:HZAU-China/Judging Form">Judging Form</a>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | <div class="HZAU_2017_menu">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/8/8e/HZAU_2017_background_1.png" class="beijin">
| + | |
| − | <ul class="daohang">
| + | |
| − | <li id="HZAU_menu_1">
| + | |
| − | <a href="#" id="bianse_1">Background</a>
| + | |
| − | </li>
| + | |
| − | <li id="HZAU_menu_2">
| + | |
| − | <a href="#item2_1" id="bianse_2">Information</a>
| + | |
| − | </li>
| + | |
| − | <li id="HZAU_menu_3">
| + | |
| − | <a href="#item3_1" id="bianse_3">Why light control</a>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | <div class="HZAU_content wrap">
| + | |
| − | <div class="item" id="item1">
| + | |
| − | <a class="biaoti">Description</a>
| + | |
| − | <a class="zhengwen">In the long history of life evolution, different species living in different environment developed the most adaptive
| + | |
| − | surviving mechanism for them under the selection pressure, among which some unmatchable become widly preserved in
| + | |
| − | different creatures, and the mechanism of replication is one of them. The majority of prokaryote share the same mechanism
| + | |
| − | of self-replication including DNA replication, chromosome seperation and cell division. These three parts comprised
| + | |
| − | the whole cell cycle, and the main model creature for researching the replication process is E.coli, which at the
| + | |
| − | same time is the main object of our project.</a>
| + | |
| − | </div>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/b/bc/HZAU_2017_tu1_1.png" class="tu_1">
| + | |
| − | <div id="item2_1" style="padding:29px"></div>
| + | |
| − | <div class="item" id="item2">
| + | |
| − | <a class="zhengwen">The replication process of E.coli replication can be devided into phase B,C and D, three phases. Phase B, also called
| + | |
| − | pre-replication phase, is for DNA replication proparation similar as G1 phase in eukaryote cell cycle. Phase C is
| + | |
| − | regarded as replication phase during which the chromosome is replicating corespond to the S phase in eukaryote. The
| + | |
| − | last phase, phase D, is called post-replication phase, in which the behave of chromosome seperation and cell division
| + | |
| − | happend just like what happened in G2 and M phase of eukaryote cells. the time of C phase and D phase in a generation
| + | |
| − | is relative constant, so what actually determines the cell cycle is the initiation of genome replication. We know
| + | |
| − | that cell have to garantee that each cell should have at least one chormosome, and this requires a highly coordination
| + | |
| − | between DNA replication and cell divsion. Though the delicate mechanism of the corrdination is still a puzzle, it
| + | |
| − | is clear that DnaA protein plays a significant role in this event. DnaA is a versatile protein, it can not only behave
| + | |
| − | like a helicase opening the duble-helix of replication initiation site, OriC, while have the function of raising
| + | |
| − | other proteins forming into the replisome to prolong the replication, but also can anchor the replicationg choromosome
| + | |
| − | on the cell membrane leading the following seperation of chromosome. But what makes it the linker between replication
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| − | and division is that its concentration determines the initiation volume of replication. So according to these information,
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| − | we think that regulating the combination of DNA and DnaA protein will be an efficient method to regulate cell replication.
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| − | <a href="">For more information please click here.</a>
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| − | </a>
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| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2017/5/51/HZAU_2017_tu2_1.png" class="tu_2">
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| − | <div id="item3_1" style="padding:29px"></div>
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| − | <div class="item" id="item3">
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| − | <a class="zhengwen">There will be about 1h for an entire genome replication, but cell division can reach about 20min per generation. this
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| − | reflects the multi-copy number of bacterial genome and only with this mechanism can E.coli reproduce in such a high
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| − | speed. what's worse is that even isogenic cells grow in the same culture have a scattering distribution not only
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| − | in phase but also in genome copy number. the strong heterogenic in cell cycle and genome copy number hinds the 3D
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| − | genome research and may be a potential noise on genome gene expression. At the same time, the developing synthetic
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| − | biology requires an approach that can control cell reproduction and can be easily incorporated into circuits, so
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| − | that the whole system can be more safe and controlable. taking these into consider, we decided to use CRISPRi to
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| − | inhibit its cell cycle, for more details please see design.</a>
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| − | </div>
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| − | <a class="ppp"></a>
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| − | <div id="item4_1" style="padding:29px"></div>
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| − | <div class="item" id="item4">
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| − | <a class="zhengwen">But barely pause the replication in some circumstance can't satisfy our needs, and long time inhibition will prolong
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| − | its length, so there is a need to totally control its replication, not only block but also open. with that purpose
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| − | we decided to use optogentic to control this system, and the operablity of light to computer makes our system programable,
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| − | which can be called 4C.</a>
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