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| − | {{Heidelberg/templateus/Mainbody|Our Project.|Foundational Advance in Peptide Synthesis.|https://static.igem.org/mediawiki/2017/a/ae/T--Heidelberg--2017_Background_Tiger.jpg|
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| − | {{Heidelberg/templateus/AbstractboxV2|TITLE|
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| − | Several secondary metabolites, such as commonly used antibiotics, pigments and detoxifying enzymes, are synthesized by non-ribosomal peptide synthetases (NRPSs). These enzymes beautifully reflect one of the fundamental principles of synthetic biology, as they are remarkably modular. We will assemble new NRPSs by combining individual domains and modules of different origin, thus setting the basis for novel and customized synthesis of non-ribosomal peptidesTo make the use of NRPSs amenable to a wider community, we will devise a new software-tool, called NRPS Designer, which predicts the optimal modular composition of synthetic NRPSs for production of any desired peptide and outputs a cloning.
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| − | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/a/ae/T--Heidelberg--2017_Background_Tiger.jpg|Eye of the Tiger|Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
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| − | Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
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| − | {{Heidelberg/templateus/Imagebox|https://static.igem.org/mediawiki/2015/thumb/4/49/Heidelberg_CLT_Fig.7_Splinted_Ligation.png/800px-Heidelberg_CLT_Fig.7_Splinted_Ligation.png|Fig: 1 Telling you something about stuff|No I´m going to describe the graphic. Hey there what´s ging on No I´m going to describe the graphic. Hey there what´s ging on|pos = left}}
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| − | Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison.
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| − | {{Heidelberg/templateus/Imagebox|https://static.igem.org/mediawiki/2015/thumb/4/49/Heidelberg_CLT_Fig.7_Splinted_Ligation.png/800px-Heidelberg_CLT_Fig.7_Splinted_Ligation.png|Fig: 1 Telling you something about stuff|No I´m going to describe the graphic. Hey there what´s ging on No I´m going to describe the graphic. Hey there what´s ging on|pos = right}}
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| − | Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
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| − | {{Heidelberg/templateus/Contentsection|
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| − | {{#tag:html|
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| − | <div class="col-lg-4 col-md-4 col-xs-12 content" style="display: inline-block;">
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| − | <h2>Buttageddon</h2>
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| − | Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork.
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| − | <button type="button" class="btn heidelbutton-red" data-toggle="button" aria-pressed="false" autocomplete="off"> FOOBAR </button>
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| − | </div>
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| − | <div class="col-lg-4 col-md-4 col-xs-12 content" style="display: inline-block;">
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| − | <h2>Formageddon</h2>
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| − | Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork.
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| − | </div>
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| − | <div class="col-lg-4 col-md-4 col-xs-12 content" style="display: inline-block;">
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| − | <h2>Listmageddon</h2>
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| − | {{Heidelberg/templateus/Orderedlist|
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| − | {{Heidelberg/templateus/Listitem|FOO}}
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| − | {{Heidelberg/templateus/Listitem|BAR}}
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| − | {{Heidelberg/templateus/Listitem|BAZ}}
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| − | {{Heidelberg/templateus/Listitem|
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| − | {{Heidelberg/templateus/Orderedlist|
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| − | {{Heidelberg/templateus/Listitem|FOOBAR}}
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| − | {{Heidelberg/templateus/Listitem|FOOBAZ}}
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| − | {{Heidelberg/templateus/Listitem|QUUX}}
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| − | {{Heidelberg/templateus/Emphasissection|
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| − | {{Heidelberg/templateus/Heading| BACON Ipsum!}}
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| − | Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison.
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| − | |color=black}}
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| − | {{Heidelberg/templateus/Emphasissection|
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| − | {{Heidelberg/templateus/Heading| BACON Ipsum!}}
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| − | Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
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| − | |color=red}}
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| − | {{Heidelberg/templateus/Contentsection|
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| − | {{Heidelberg/templateus/Tablebox| IMATABLE |
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| − | {{#tag:html|
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| − | <table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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| − | <thead>
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| − | <tr>
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| − | <th>Step</th>
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| − | <th>Temperature (°C)</th>
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| − | <th>Time</th>
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| − | </tr>
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| − | </thead>
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| − | <tbody>
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| − | <td>Initial denaturation</td>
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| − | <td>98</td>
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| − | <td>30 seconds</td>
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| − | </tr>
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| − | <tr>
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| − | <td>25-35 cycles</td>
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| − | <td>98 (denaturation)<br>
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| − | 45-72 (annealing) <a href=""#Note1">see" Note 1</a><br>
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| − | 72 (extension)</td>
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| − | <td>5-10 seconds <br>
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| − | 10-30 seconds<br>
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| − | 15-30 seconds per kb</td>
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| − | </tr>
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| − | <tr>
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| − | <td>Final extension</td>
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| − | <td>72</td>
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| − | <td>2-5 minutes</td>
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| − | </tr>
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| − | <tr>
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| − | <td>Hold</td>
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| − | <td>4</td>
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| − | <td>Indefinitely</td>
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| − | </tr>
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| − | </tbody>
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| − | </table>
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| − | }} |
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | Here you can see a whole lot of shite in a table.
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| − | <div class="row content">
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| − | <h3> References </h3>
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| − | <p id="references"></p>
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| − | <p>[1]<a>Here comes a link</a></p>
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| − | </div>
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